EC Number   |
Substrates |
Organism |
Products  |
Reversibility |
|---|
   2.8.1.4 | more |
Ctu1 (cytosolic tRNA thiouridilase) associates with the conserved Ctu2 protein and this complex is the wobble uridine-34 thiolase of cytosolic tRNAs in eukaryotes, 2-thiouridylation of tRNAs |
Schizosaccharomyces pombe |
? |
- |
? |
| 2.8.1.4 | more |
tRNA (LYS), but not the tRNA (MET), which has a C at position 34, is thiolated in vivo |
Caenorhabditis elegans |
? |
- |
? |
| 2.8.1.4 | more |
tRNA (LYS), but not the tRNA (MET), which has a C at position 34, is thiolated in vivo |
Schizosaccharomyces pombe |
? |
- |
? |
| 2.8.1.4 | more |
only transfer RNA as sulfur acceptor, other cellular species of RNA and synthetic ribohomopolymers do not accept sulfur in this system, no reaction with E. coli rRNA, phage MS2 RNA, polyC, polyA, or polyU |
Bacillus subtilis W168 |
? |
- |
? |
| 2.8.1.4 | more |
2-thiocytidine is the major thionucleotide formed in vitro, 4-thiouridine is the major product in vivo |
Escherichia coli HfrC |
? |
- |
? |
| 2.8.1.4 | 3-mercaptopyruvate + activated tRNA |
- |
Bacillus subtilis |
? + S-tRNA |
- |
? |
| 2.8.1.4 | 3-mercaptopyruvate + activated tRNA |
in vitro |
Rattus norvegicus |
? + S-tRNA |
- |
? |
| 2.8.1.4 | 3-mercaptopyruvate + activated tRNA |
reaction product is sensitive to deacylation |
Rattus norvegicus |
? + S-tRNA |
- |
? |
| 2.8.1.4 | L-cysteine + denatured DNA |
DNA prepared from various sources appears to function as a good sulfur acceptor |
Bacillus subtilis |
L-serine + ? |
- |
? |
| 2.8.1.4 | L-cysteine + poly A-2poly U |
- |
Escherichia coli |
L-serine + S-tRNA |
- |
? |
