EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.5.1.59 | more |
most of the CaaX-containing proteins with X= Leu and X= Met are good substrates of PGGT-I, whereas those with X= Phe are poor substrates for PGGT-I, Trypanosma cruzi j2 DNAJ protein, Trypanosoma cruzi PRL-1 protein tyrosine phosphatase, CHFM (a small GTPase like protein), and Trypanosoma cruzi Rho1 GTPase are no substrates for PGGT-I |
Trypanosoma cruzi |
? |
- |
? |
2.5.1.59 | more |
the enzyme modifies proteins by attaching a 20-carbon isoprenoid group to a cysteine residue near the C-terminus of a target protein. The enzyme requires a C-terminal Ca1a2X sequence on its substrates, with the a1, a2, and X residues serving as substrate-recognition elements for GGTase-I. Crystallographic structures of rat GGTase-I show a tightly packed and hydrophobic a2 residue binding pocket, consistent with a preference for moderately sized a2 residues in GGTase-I substrates, peptide substrate structure-activity relationship, overview. Identification of specific active-site residues within rat GGTase-I involved in substrate recognition |
Rattus norvegicus |
? |
- |
? |
2.5.1.59 | more |
GGTase-I can transfer isoprenoids to intracellular proteins that contain CAAX motifs. The isoprenoid groups can be selectively recognized by GGTase-I. The 20-carbon isoprenoid geranylgeranyl from its donor geranylgeranyl diphosphate (GGPP) is specific to GGTase-I. No activity with H-Ras |
Homo sapiens |
? |
- |
? |
2.5.1.59 | more |
GGTase-I can transfer isoprenoids to intracellular proteins that contain CAAX motifs. The isoprenoid groups can be selectively recognized by GGTase-I. The 20-carbon isoprenoid geranylgeranyl from its donor geranylgeranyl diphosphate (GGPP) is specific to GGTase-I. Residues Lysalpha164, Hisbeta219, Argbeta263, Lysbeta266 and Tyrbeta272 in GGTase-I can form hydrogen bonds with GGPP. No activity with H-Ras |
Rattus norvegicus |
? |
- |
? |
2.5.1.59 | more |
the enzyme has a restricted substrate specificity. Ggtase-I substrates often contain CaaL motifs, overview. Of the three Rho paralogues, only GFP-Rho10 localizes to cellular membranes in wild-type cells. This membrane localization is slightly disrupted in the cdc43DELTA mutant, indicating that the Rho10 protein is likely a Ggtase-I substrate |
Cryptococcus neoformans |
? |
- |
? |
2.5.1.59 | more |
enzyme prefers farnesyl diphosphate over geranylgeranyl diphosphate, but catalyzes the reactions of both substrates, i.e. reactions of EC 2.5.1.58 and EC 2.5.1.59, respectively |
Rattus norvegicus |
? |
- |
- |
2.5.1.59 | Ser-Ser-Cys-Ile-Leu-Leu + geranylgeranyl diphosphate |
- |
Bos taurus |
diphosphate + Ser-Ser-S-geranylgeranyl-Cys-Ile-Leu-Leu |
- |
? |