2.5.1.59 3,7,11-trimethyl-12-(7-nitro-benzo[1,2,5]-oxadiazo-4-ylamino)-dodeca-2,6,10-trien-1-diphosphate + GST-RhoA - Homo sapiens diphosphate + ? - ? 387322 2.5.1.59 3,7-dimethyl-8-(7-nitro-benzo[1,2,5]oxadiazol-4-ylamino)-octa-2,6-diene-1-diphosphate + GST-RhoA - Homo sapiens diphosphate + ? - ? 387323 2.5.1.59 farnesyl diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu biotin-gamma6 Bos taurus diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFF-S-farnesyl-CAI-Leu - ? 353429 2.5.1.59 farnesyl diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu ECB-gamma6 Bos taurus diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFF-S-farnesyl-CAI-Leu - ? 353429 2.5.1.59 farnesyl diphosphate + Ki-Ras4B - Homo sapiens diphosphate + S-geranylgeranyl-Ki-Ras4B - ? 353433 2.5.1.59 farnesyl diphosphate + protein-cysteine the enzyme catalyzes postttranslational modification of proteins, the farnesyl moieties attached to the substrates are direcly involved in protein-protein interactions as well as in protein-membrane interactions Homo sapiens S-farnesyl protein + diphosphate - ? 370439 2.5.1.59 farnesyl diphosphate + protein-cysteine substrate motif: carboxy-terminal -Ca1a2X box Homo sapiens S-farnesyl protein + diphosphate - ? 370439 2.5.1.59 geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-ALEPPETEPKRKCCIF - Bos taurus diphosphate + (biotin-CONH-(CH2)5-CO-)-ALEPPETEPKRK-S-geranylgeranyl-CCIF - ? 353427 2.5.1.59 geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-ALEPPETEPKRKCCIF ECB-G25K, 42% activity in comparison of protein substrate motif: CAA-leucine Bos taurus diphosphate + (biotin-CONH-(CH2)5-CO-)-ALEPPETEPKRK-S-geranylgeranyl-CCIF - ? 353427 2.5.1.59 geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-GTPRASNRSCAIS ECB-laminB(S), 5% activity in comparison of protein substrate motif: CAA-leucine Bos taurus diphosphate + (biotin-CONH-(CH2)5-CO-)-GTPRASNRS-S-geranylgeranyl-CAIS - ? 353428 2.5.1.59 geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu biotin-gamma6, preferred substrate Bos taurus diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFF-S-geranylgeranyl-CAI-Leu - ? 353426 2.5.1.59 geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu ECB-gamma6, preferred substrate Bos taurus diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFF-S-geranylgeranyl-CAI-Leu - ? 353426 2.5.1.59 geranylgeranyl diphosphate + Arg-Arg-Cys-Val-Leu-Leu - Rattus norvegicus diphosphate + Arg-Arg-S-geranylgeranyl-Cys-Val-Leu-Leu - ? 353438 2.5.1.59 geranylgeranyl diphosphate + Asp-Asp-Pro-Thr-Ala-Ser-Ala-Cys-Val-Leu-Leu - Homo sapiens Asp-Asp-Pro-Thr-Ala-Ser-Ala-(S-geranylgeranyl)-Cys-Val-Leu-Leu + diphosphate - ? 388754 2.5.1.59 geranylgeranyl diphosphate + Cdc42-cysteine - Rattus norvegicus S-geranylgeranyl-Cdc42 + diphosphate - ? 436471 2.5.1.59 geranylgeranyl diphosphate + Cdc42-cysteine - Cryptococcus neoformans S-geranylgeranyl-Cdc42 + diphosphate - ? 436471 2.5.1.59 geranylgeranyl diphosphate + Cdc42-cysteine - Rattus norvegicus Sprague-Dawley S-geranylgeranyl-Cdc42 + diphosphate - ? 436471 2.5.1.59 geranylgeranyl diphosphate + Cdc420-cysteine - Cryptococcus neoformans S-geranylgeranyl-Cdc420 + diphosphate - ? 436472 2.5.1.59 geranylgeranyl diphosphate + Cys-Val-Leu-Leu - Rattus norvegicus S-geranylgeranyl-Cys-Val-Leu-Leu + diphosphate - ? 388755 2.5.1.59 geranylgeranyl diphosphate + Cys-Val-Leu-Ser - Rattus norvegicus S-geranylgeranyl-Cys-Val-Leu-Ser + diphosphate - ? 388756 2.5.1.59 geranylgeranyl diphosphate + dansyl-Cys-Ile-Ile-Leu - Saccharomyces cerevisiae diphosphate + dansyl-S-geranylgeranyl-Cys-Ile-Ile-Leu - ? 353436 2.5.1.59 geranylgeranyl diphosphate + dansyl-GCFT - Rattus norvegicus diphosphate + S-geranylgeranyl dansyl-GCFT - ? 457996 2.5.1.59 geranylgeranyl diphosphate + dansyl-GCII - Rattus norvegicus diphosphate + S-geranylgeranyl dansyl-GCII - ? 457997 2.5.1.59 geranylgeranyl diphosphate + dansyl-GCLL - Rattus norvegicus diphosphate + S-geranylgeranyl dansyl-GCLL - ? 457998 2.5.1.59 geranylgeranyl diphosphate + dansyl-GCYL - Rattus norvegicus diphosphate + S-geranylgeranyl dansyl-GCYL - ? 457999 2.5.1.59 geranylgeranyl diphosphate + dansyl-Gly-Cys-Ile-Ile-Leu - Saccharomyces cerevisiae dansyl-Gly-(S-geranylgeranyl)-Cys-Ile-Ile-Leu + diphosphate - ? 452211 2.5.1.59 geranylgeranyl diphosphate + dansyl-Thr-Lys-Cys-Val-Ile-Met - Escherichia coli diphosphate + dansyl-Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Met - ? 388757 2.5.1.59 geranylgeranyl diphosphate + GST-CDC42 recombinant glutathione S-transferase fusion protein of CDC42Hs with a C-terminal Cys-Cys-Ile-Phe sequence Bos taurus diphosphate + GST-S-geranylgeranyl-CDC42 - ? 353430 2.5.1.59 geranylgeranyl diphosphate + GST-RhoA - Homo sapiens diphosphate + GST-S-geranylgeranyl-RhoA - ? 388758 2.5.1.59 geranylgeranyl diphosphate + H-Ras-CVLL - Homo sapiens S-geranylgeranyl-protein + ? - ? 388759 2.5.1.59 geranylgeranyl diphosphate + K Ras-cysteine - Rattus norvegicus S-geranylgeranyl-K Ras + diphosphate - ? 436474 2.5.1.59 geranylgeranyl diphosphate + K Ras-cysteine - Homo sapiens S-geranylgeranyl-K Ras + diphosphate - ? 436474 2.5.1.59 geranylgeranyl diphosphate + Ki-Ras4A - Homo sapiens diphosphate + S-geranylgeranyl-Ki-Ras4A - ? 353434 2.5.1.59 geranylgeranyl diphosphate + Ki-Ras4B - Escherichia coli diphosphate + S-geranylgeranyl-Ki-Ras4B - ? 388761 2.5.1.59 geranylgeranyl diphosphate + Ki-Ras4B - Homo sapiens diphosphate + S-geranylgeranyl-Ki-Ras4B - ? 388761 2.5.1.59 geranylgeranyl diphosphate + Leu-Pro-Cys-Val-Val-Met - Rattus norvegicus diphosphate + Leu-Pro-S-geranylgeranyl-Cys-Val-Val-Met - ? 353441 2.5.1.59 geranylgeranyl diphosphate + Lys-Lys-Cys-Ile-Ile-Met - Rattus norvegicus diphosphate + Lys-Lys-S-geranylgeranyl-Cys-Ile-Ile-Met - ? 353439 2.5.1.59 geranylgeranyl diphosphate + Lys-Pro-Cys-Val-Val-Met - Rattus norvegicus Lys-Pro-(S-geranylgeranyl)-Cys-Val-Val-Met + diphosphate - ? 452212 2.5.1.59 geranylgeranyl diphosphate + N-dansyl-Gly-Cys-Val-Ile-Leu-OH - Rattus norvegicus ? - ? 409505 2.5.1.59 geranylgeranyl diphosphate + N-Ras - Homo sapiens diphosphate + S-geranylgeranyl-N-Ras - ? 353435 2.5.1.59 geranylgeranyl diphosphate + N-Ras-cysteine - Rattus norvegicus S-geranylgeranyl-N-Ras + diphosphate - ? 436475 2.5.1.59 geranylgeranyl diphosphate + N-Ras-cysteine - Homo sapiens S-geranylgeranyl-N-Ras + diphosphate - ? 436475 2.5.1.59 geranylgeranyl diphosphate + Phe-Phe-Cys-Ala-Ile-Leu - Rattus norvegicus diphosphate + Phe-Phe-S-geranylgeranyl-Cys-Ala-Ile-Leu - ? 353440 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine - Mus musculus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine - Homo sapiens S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine - Rattus norvegicus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine - Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine - Bos taurus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine - Catharanthus roseus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine - Candida albicans S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine - Trypanosoma cruzi S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine - Cryptococcus neoformans S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu Homo sapiens S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu Bos taurus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine the Rab protein Rab8-GTPase, which ends with a Cys-Val-Leu-Leu motif is able to serve as a substrate for either geranylgeranyl transferase I and II, but modified predominantly by geranylgeranyl transferase II, Y78D mutation selective prevents prenylation of Rab8 by geranylgeranyl transferase II, but not geranylgeranyltransferaseI Rattus norvegicus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine prenylation, substrates are Rho, Rac, most trimeric G protein gamma subunits Rattus norvegicus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine prenylation, substrates are Rho, Rac, most trimeric G protein gamma subunits Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X is Leu, Phe or Ser, preferred substrate: Leu Rattus norvegicus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X is Leu, Phe or Ser, preferred substrate: Leu Bos taurus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X is Leu or Phe Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Homo sapiens S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Rattus norvegicus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Bos taurus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Schizosaccharomyces pombe S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X may be Leu, Phe, or Ser, with high concentrations of peptide substrates this specificity is lost Bos taurus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme catalyses the transfer of the 20-carbon prenyl group from geranylgeranyl diphosphate to the cysteine residue near the C-termini of a variety of eukaryotic proteins Bos taurus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine this prenylation is necessary for many proteins to interact with membrane localized at proper intracellular sites Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine this prenylation is necessary for many proteins to interact with membrane localized at proper intracellular sites Schizosaccharomyces pombe S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine yeast enzyme is essential for yeast cell growth Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine GGTase-I catalyzes C-terminal lipidation of more than 100 proteins, including many GTP-binding regulatory proteins Rattus norvegicus S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine the enzyme negatively regulates abscisic acid signaling in guard cells. The enzyme is specifically involved in negative regulation of auxin-induced lateral root initiation Arabidopsis thaliana S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine substrate motif: carboxy-terminal -Ca1a2X box Homo sapiens S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine the enzyme adds a C20 geranylgeranyl group to proteins such as RhoA, RhoC, Rap1 and Ral at the cysteine within the carboxy-terminal tetrapeptide consensus sequence CAAL (C is cysteine, A is an aliphatic amino acid, and the C-terminal residue is leucine or phenylalanine) Homo sapiens S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine - Rattus norvegicus Sprague-Dawley S-geranylgeranyl-protein + diphosphate - ? 353424 2.5.1.59 geranylgeranyl diphosphate + protein-cysteine the enzyme catalyzes posttranslational modification of proteins, the farnesyl moieties attached to the substrates are direcly involved in protein-protein interactions as well as in protein-membrane interactions Homo sapiens S-geranylgeranyl protein + diphosphate - ? 375135 2.5.1.59 geranylgeranyl diphosphate + Rac GTPase-cysteine - Rattus norvegicus S-geranylgeranyl-Rac GTPase + diphosphate - ? 436476 2.5.1.59 geranylgeranyl diphosphate + Rac-cysteine - Rattus norvegicus S-geranylgeranyl-Rac + diphosphate - ? 436477 2.5.1.59 geranylgeranyl diphosphate + Rac-cysteine - Homo sapiens S-geranylgeranyl-Rac + diphosphate - ? 436477 2.5.1.59 geranylgeranyl diphosphate + Rac-cysteine - Rattus norvegicus Sprague-Dawley S-geranylgeranyl-Rac + diphosphate - ? 436477 2.5.1.59 geranylgeranyl diphosphate + Rap-cysteine - Rattus norvegicus S-geranylgeranyl-Rap + diphosphate - ? 436478 2.5.1.59 geranylgeranyl diphosphate + Rap-cysteine - Homo sapiens S-geranylgeranyl-Rap + diphosphate - ? 436478 2.5.1.59 geranylgeranyl diphosphate + Rap1A-cysteine - Rattus norvegicus S-geranylgeranyl-Rap1A + diphosphate - ? 436479 2.5.1.59 geranylgeranyl diphosphate + Rap1A-cysteine - Homo sapiens S-geranylgeranyl-Rap1A + diphosphate - ? 436479 2.5.1.59 geranylgeranyl diphosphate + Rap1A-cysteine - Rattus norvegicus Sprague-Dawley S-geranylgeranyl-Rap1A + diphosphate - ? 436479 2.5.1.59 geranylgeranyl diphosphate + Ras protein - Homo sapiens S-geranylgeranyl-Ras protein + diphosphate - ? 410842 2.5.1.59 geranylgeranyl diphosphate + Ras protein - Sus scrofa S-geranylgeranyl-Ras protein + diphosphate - ? 410842 2.5.1.59 geranylgeranyl diphosphate + Ras-CVLL tritium labelled geranylgeranyl diphosphate for enzyme assay, His-tagged geranylgeranyl diphosphate Mammalia S-geranylgeranyl-Ras-CVLL + diphosphate - ? 410843 2.5.1.59 geranylgeranyl diphosphate + Ras-Cys-Val-Leu-Leu - Homo sapiens diphosphate + Ras-S-geranylgeranyl-Cys-Val-Leu-Leu - ? 353431 2.5.1.59 geranylgeranyl diphosphate + Ras-Cys-Val-Leu-Leu - Rattus norvegicus diphosphate + Ras-S-geranylgeranyl-Cys-Val-Leu-Leu - ? 353431 2.5.1.59 geranylgeranyl diphosphate + Rho GTPase-cysteine - Rattus norvegicus S-geranylgeranyl-Rho GTPase + diphosphate - ? 436480 2.5.1.59 geranylgeranyl diphosphate + Rho GTPase-cysteine - Rattus norvegicus Sprague-Dawley S-geranylgeranyl-Rho GTPase + diphosphate - ? 436480 2.5.1.59 geranylgeranyl diphosphate + Rho-cysteine - Rattus norvegicus S-geranylgeranyl-Rho + diphosphate - ? 436481 2.5.1.59 geranylgeranyl diphosphate + Rho-cysteine - Homo sapiens S-geranylgeranyl-Rho + diphosphate - ? 436481 2.5.1.59 geranylgeranyl diphosphate + Rho10-cysteine - Cryptococcus neoformans S-geranylgeranyl-Rho10 + diphosphate - ? 436482 2.5.1.59 geranylgeranyl diphosphate + Rho10-cysteine while Rho10 has benn previously predicted to lack a CaaX motif, analysis of the Rho10 transcript sequence using RNA sequencing data reveals that one splice site is misannotated, the corrected sequence contains a CLIL C-terminal motif Cryptococcus neoformans S-geranylgeranyl-Rho10 + diphosphate - ? 436482 2.5.1.59 geranylgeranyl diphosphate + RhoA - Homo sapiens diphosphate + S-geranylgeranyl-RhoA - ? 388763 2.5.1.59 geranylgeranyl diphosphate + RhoA GTPase-cysteine - Rattus norvegicus S-geranylgeranyl-RhoA GTPase + diphosphate - ? 436483 2.5.1.59 geranylgeranyl diphosphate + rhoC protein - Homo sapiens S-geranylgeranyl-protein + diphosphate - ? 397254 2.5.1.59 geranylgeranyl diphosphate + S-geranylgeranyl-Ki-Ras4B both the polylysine and the carboxy-terminal methionine are important for geranylgeranylation of this substrate Homo sapiens diphosphate + S-geranylgeranyl-Ki-Ras4B - ? 353432 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Ala - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Ala - ? 388764 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Arg - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Arg - ? 388765 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Asn - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Asn - ? 388766 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Asp - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Asp - ? 388767 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Cys - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Cys - ? 388768 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Gln - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Gln - ? 388769 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Ile - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Ile - ? 388770 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Leu - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Leu - ? 388771 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Met - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Met - ? 388772 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Phe - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Phe - ? 388773 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Ser - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Ser - ? 388774 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Thr - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Thr - ? 388775 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Tyr - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Tyr - ? 388776 2.5.1.59 geranylgeranyl diphosphate + Thr-Lys-Cys-Val-Ile-Val - Escherichia coli diphosphate + Thr-Lys-(S-geranylgeranyl)-Cys-Val-Ile-Val - ? 388777 2.5.1.59 geranylgeranyl diphosphate + [glutathione S-transferase]-GCVKIKKCVIL - Saccharomyces cerevisiae diphosphate + ? - ? 353437 2.5.1.59 geranylgeranyl diphosphate + [glutathione S-transferase]-GCVKIKKCVIL - Bos taurus diphosphate + ? - ? 353437 2.5.1.59 additional information requires geranylgeranylation of rho1p for the correct function of enzyme Schizosaccharomyces pombe ? - ? 89 2.5.1.59 additional information not: N-Ras Homo sapiens ? - ? 89 2.5.1.59 additional information some of the 18 analysed hexapeptides effieciently farnsylated by enzyme Rattus norvegicus ? - ? 89 2.5.1.59 additional information most of the CaaX-containing proteins with X= Leu and X= Met are good substrates of PGGT-I, whereas those with X= Phe are poor substrates for PGGT-I, Trypanosma cruzi j2 DNAJ protein, Trypanosoma cruzi PRL-1 protein tyrosine phosphatase, CHFM (a small GTPase like protein), and Trypanosoma cruzi Rho1 GTPase are no substrates for PGGT-I Trypanosoma cruzi ? - ? 89 2.5.1.59 additional information the enzyme modifies proteins by attaching a 20-carbon isoprenoid group to a cysteine residue near the C-terminus of a target protein. The enzyme requires a C-terminal Ca1a2X sequence on its substrates, with the a1, a2, and X residues serving as substrate-recognition elements for GGTase-I. Crystallographic structures of rat GGTase-I show a tightly packed and hydrophobic a2 residue binding pocket, consistent with a preference for moderately sized a2 residues in GGTase-I substrates, peptide substrate structure-activity relationship, overview. Identification of specific active-site residues within rat GGTase-I involved in substrate recognition Rattus norvegicus ? - ? 89 2.5.1.59 additional information GGTase-I can transfer isoprenoids to intracellular proteins that contain CAAX motifs. The isoprenoid groups can be selectively recognized by GGTase-I. The 20-carbon isoprenoid geranylgeranyl from its donor geranylgeranyl diphosphate (GGPP) is specific to GGTase-I. No activity with H-Ras Homo sapiens ? - ? 89 2.5.1.59 additional information GGTase-I can transfer isoprenoids to intracellular proteins that contain CAAX motifs. The isoprenoid groups can be selectively recognized by GGTase-I. The 20-carbon isoprenoid geranylgeranyl from its donor geranylgeranyl diphosphate (GGPP) is specific to GGTase-I. Residues Lysalpha164, Hisbeta219, Argbeta263, Lysbeta266 and Tyrbeta272 in GGTase-I can form hydrogen bonds with GGPP. No activity with H-Ras Rattus norvegicus ? - ? 89 2.5.1.59 additional information the enzyme has a restricted substrate specificity. Ggtase-I substrates often contain CaaL motifs, overview. Of the three Rho paralogues, only GFP-Rho10 localizes to cellular membranes in wild-type cells. This membrane localization is slightly disrupted in the cdc43DELTA mutant, indicating that the Rho10 protein is likely a Ggtase-I substrate Cryptococcus neoformans ? - ? 89 2.5.1.59 additional information enzyme prefers farnesyl diphosphate over geranylgeranyl diphosphate, but catalyzes the reactions of both substrates, i.e. reactions of EC 2.5.1.58 and EC 2.5.1.59, respectively Rattus norvegicus ? - - 89 2.5.1.59 Ser-Ser-Cys-Ile-Leu-Leu + geranylgeranyl diphosphate - Bos taurus diphosphate + Ser-Ser-S-geranylgeranyl-Cys-Ile-Leu-Leu - ? 353425