EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.4.1.362 | more |
enzyme GtfZ-D2 produces partially branched polymers. Analysis of the glucosyl linkage composition of the (alpha1->3)-branched polymers. Reaction product analyses with 1D 1H NMR, overview. GtfZ-CD2 of Lactobacillus kunkeei uses sucrose as a glucose donor to decorate dextran molecules, adding single (alpha1->3)-branched glucose units on the linear (alpha1->6) glucose chain. GtfZ-CD2 also exhibits (alpha1->3)-branching activity on IMMP (a product of reaction of GtfB, EC 2.4.1.B52, with about 90% (alpha1->6)-linkages) |
Apilactobacillus kunkeei DSM 12361 |
? |
- |
- |
2.4.1.362 | more |
alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages |
Leuconostoc citreum NRRL B-1299 |
? |
- |
- |
2.4.1.362 | sucrose |
- |
Leuconostoc citreum |
D-glucose + leucrose |
- |
? |
2.4.1.362 | sucrose |
- |
Leuconostoc citreum NRRL B-742 |
D-glucose + leucrose |
- |
? |
2.4.1.362 | sucrose + (1->6)-alpha-D-glucan |
- |
Leuconostoc citreum |
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch |
- |
? |
2.4.1.362 | sucrose + (1->6)-alpha-D-glucan |
- |
Apilactobacillus kunkeei |
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch |
- |
? |
2.4.1.362 | sucrose + (1->6)-alpha-D-glucan |
transglucosylation activity |
Apilactobacillus kunkeei |
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch |
- |
? |
2.4.1.362 | sucrose + (1->6)-alpha-D-glucan |
- |
Leuconostoc citreum NRRL B-742 |
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch |
- |
? |
2.4.1.362 | sucrose + (1->6)-alpha-D-glucan |
- |
Apilactobacillus kunkeei DSM 12361 |
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch |
- |
? |
2.4.1.362 | sucrose + (1->6)-alpha-D-glucan |
transglucosylation activity |
Apilactobacillus kunkeei DSM 12361 |
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch |
- |
? |