2.4.1.362	dextran + sucrose	dextran Mw is 70 kDa, transglucosylation activity	Apilactobacillus kunkeei	D-fructose + dextran containing a (1->3)-alpha-D-glucose branch	-	?	457899	
2.4.1.362	dextran + sucrose	dextran Mw is 70 kDa, transglucosylation activity	Apilactobacillus kunkeei DSM 12361	D-fructose + dextran containing a (1->3)-alpha-D-glucose branch	-	?	457899	
2.4.1.362	isomalto/malto-polysaccharide + sucrose	alpha(1->3)-branched IMMP, IMMP Mw is 70 kDa, transglucosylation activity	Apilactobacillus kunkeei	D-fructose + isomalto/malto-polysaccharide containing a (1->3)-alpha-D-glucose branch	-	?	458050	
2.4.1.362	additional information	domain CD2 additionally displays hydrolytic acivity on dextran	Apilactobacillus kunkeei	?	-	?	89	
2.4.1.362	additional information	enzyme harbors two separate catalytic cores (CD1 and CD2), predicted to have glucansucrase and branching sucrase specificity, respectively. CD2 displays an efficient transglycosidase activity with sucrose as donor substrate. The CD2-catalyzed transglycosylation reaction follows a Ping Pong Bi Bi mechanism and produces single glucosyl (alpha1->3) linked branches onto dextran, resulting in the production of highly branched comb-like alpha-glucan products	Apilactobacillus kunkeei	?	-	?	89	
2.4.1.362	additional information	alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages	Leuconostoc citreum	?	-	-	89	
2.4.1.362	additional information	enzyme GtfZ-D2 produces partially branched polymers. Analysis of the glucosyl linkage composition of the (alpha1->3)-branched polymers. Reaction product analyses with 1D 1H NMR, overview. GtfZ-CD2 of Lactobacillus kunkeei uses sucrose as a glucose donor to decorate dextran molecules, adding single (alpha1->3)-branched glucose units on the linear (alpha1->6) glucose chain. GtfZ-CD2 also exhibits (alpha1->3)-branching activity on IMMP (a product of reaction of GtfB, EC 2.4.1.B52, with about 90% (alpha1->6)-linkages)	Apilactobacillus kunkeei	?	-	-	89	
2.4.1.362	additional information	alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages	Leuconostoc citreum NRRL B-742	?	-	-	89	
2.4.1.362	additional information	domain CD2 additionally displays hydrolytic acivity on dextran	Apilactobacillus kunkeei DSM 12361	?	-	?	89	
2.4.1.362	additional information	enzyme harbors two separate catalytic cores (CD1 and CD2), predicted to have glucansucrase and branching sucrase specificity, respectively. CD2 displays an efficient transglycosidase activity with sucrose as donor substrate. The CD2-catalyzed transglycosylation reaction follows a Ping Pong Bi Bi mechanism and produces single glucosyl (alpha1->3) linked branches onto dextran, resulting in the production of highly branched comb-like alpha-glucan products	Apilactobacillus kunkeei DSM 12361	?	-	?	89	
2.4.1.362	additional information	enzyme GtfZ-D2 produces partially branched polymers. Analysis of the glucosyl linkage composition of the (alpha1->3)-branched polymers. Reaction product analyses with 1D 1H NMR, overview. GtfZ-CD2 of Lactobacillus kunkeei uses sucrose as a glucose donor to decorate dextran molecules, adding single (alpha1->3)-branched glucose units on the linear (alpha1->6) glucose chain. GtfZ-CD2 also exhibits (alpha1->3)-branching activity on IMMP (a product of reaction of GtfB, EC 2.4.1.B52, with about 90% (alpha1->6)-linkages)	Apilactobacillus kunkeei DSM 12361	?	-	-	89	
2.4.1.362	additional information	alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages	Leuconostoc citreum NRRL B-1299	?	-	-	89	
2.4.1.362	sucrose	-	Leuconostoc citreum	D-glucose + leucrose	-	?	448144	
2.4.1.362	sucrose	-	Leuconostoc citreum NRRL B-742	D-glucose + leucrose	-	?	448144	
2.4.1.362	sucrose + (1->6)-alpha-D-glucan	-	Leuconostoc citreum	D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	457267	
2.4.1.362	sucrose + (1->6)-alpha-D-glucan	-	Apilactobacillus kunkeei	D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	457267	
2.4.1.362	sucrose + (1->6)-alpha-D-glucan	transglucosylation activity	Apilactobacillus kunkeei	D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	457267	
2.4.1.362	sucrose + (1->6)-alpha-D-glucan	-	Leuconostoc citreum NRRL B-742	D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	457267	
2.4.1.362	sucrose + (1->6)-alpha-D-glucan	-	Apilactobacillus kunkeei DSM 12361	D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	457267	
2.4.1.362	sucrose + (1->6)-alpha-D-glucan	transglucosylation activity	Apilactobacillus kunkeei DSM 12361	D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	457267	
2.4.1.362	sucrose + (1->6)-alpha-D-glucan	-	Leuconostoc citreum NRRL B-1299	D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	457267	
2.4.1.362	sucrose + dextran	-	Leuconostoc citreum	D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	448141	
2.4.1.362	sucrose + dextran	dextran of 70 kDa	Leuconostoc citreum	D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	448141	
2.4.1.362	sucrose + dextran	dextran of 70 kDa	Apilactobacillus kunkeei	D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	448141	
2.4.1.362	sucrose + dextran	-	Leuconostoc citreum NRRL B-742	D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	448141	
2.4.1.362	sucrose + dextran	dextran of 70 kDa	Leuconostoc citreum NRRL B-742	D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	448141	
2.4.1.362	sucrose + dextran	dextran of 70 kDa	Apilactobacillus kunkeei DSM 12361	D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch	-	?	448141	
2.4.1.362	sucrose + H2O	sucrose hydrolysis	Apilactobacillus kunkeei	D-fructose + D-glucose	-	?	94154	
2.4.1.362	sucrose + H2O	sucrose hydrolysis	Apilactobacillus kunkeei DSM 12361	D-fructose + D-glucose	-	?	94154