EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
3.5.1.19 | 0.0086 |
- |
1-methylnicotinamide |
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C |
718924 |
3.5.1.19 | 0.0096 |
- |
nicotinamide |
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C |
718924 |
3.5.1.19 | 0.025 |
- |
Benzamide |
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C |
718924 |
3.5.1.19 | 0.05 |
- |
ethyl nicotinate |
recombinant mutant Q96K, pH 7.3, 37°C |
735084 |
3.5.1.19 | 0.05 |
- |
Thionicotinamide |
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C |
718924 |
3.5.1.19 | 0.061 |
- |
5-Methylnicotinamide |
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C |
718924 |
3.5.1.19 | 0.1 |
- |
ethyl nicotinate |
recombinant wild-type enzyme, pH 7.3, 37°C |
735084 |
3.5.1.19 | 0.1 |
- |
ethylnicotinate |
37°C, pH 7.3, mutant enzyme W83F |
755150 |
3.5.1.19 | 0.13 |
- |
ethyl nicotinate |
recombinant mutant Q96A, pH 7.3, 37°C |
735084 |
3.5.1.19 | 0.157 |
- |
Pyrazinamide |
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C |
718924 |