BRENDA - Enzyme Database show
show all sequences of 3.5.1.19

Biochemical and mutational analysis of a novel nicotinamidase from Oceanobacillus iheyensis HTE831

Sanchez-Carron, G.; Garcia-Garcia, M.I.; Zapata-Perez, R.; Takami, H.; Garcia-Carmona, F.; Sanchez-Ferrer, A.; PLoS ONE 8, e56727 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta 2 (DE3)
Oceanobacillus iheyensis
Engineering
Amino acid exchange
Commentary
Organism
C133A
site-directed mutagenesis, the mutation affectes the active site cavity, and enhances the binding of pyrazinamide compared to the wild-type enzyme
Oceanobacillus iheyensis
E65H
site-directed mutagenesis, the mutation highly reduces the activity with pyrazinamide compared to the wild-type enzyme
Oceanobacillus iheyensis
F68W
site-directed mutagenesis, the mutation affectes the active site cavity, and enhances the binding of pyrazinamide compared to the wild-type enzyme
Oceanobacillus iheyensis
F68W/C133A
site-directed mutagenesis, the mutation affectes the active site cavity, and enhances the binding of pyrazinamide compared to the wild-type enzyme
Oceanobacillus iheyensis
K104A
site-directed mutagenesis, the mutant shows 98.55% reduced activity compared to the wild-type enzyme
Oceanobacillus iheyensis
additional information
mutation of residues Cys133 and Phe68 is required for increasing pyrazinamidase activity 2.9 and 2.5fold, respectively. In addition, the change in the fourth residue involved in the ion metal binding (Glu65) is detrimental to pyrazinamidase activity decreasing it 6fold
Oceanobacillus iheyensis
Q96A
site-directed mutagenesis, the mutant shows similar activity with nicotinamide and increased activity with 5-methyl nicotinamide compared to the wild-type enzyme
Oceanobacillus iheyensis
Q96K
site-directed mutagenesis, the mutant shows 62.7% reduced activity compared to the wild-type enzyme
Oceanobacillus iheyensis
T12Q
site-directed mutagenesis, the mutant shows 98.42% reduced activity compared to the wild-type enzyme
Oceanobacillus iheyensis
Inhibitors
Inhibitors
Commentary
Organism
Structure
5-bromonicotinaldehyde
competitive inhibition
Oceanobacillus iheyensis
nicotinaldehyde
competitive inhibition
Oceanobacillus iheyensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Oceanobacillus iheyensis
0.13
-
nicotinamide
recombinant mutant E65H, pH 7.3, 37°C
Oceanobacillus iheyensis
0.17
-
nicotinamide
recombinant mutant F68W, pH 7.3, 37°C
Oceanobacillus iheyensis
0.19
-
nicotinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.26
-
nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.34
-
ethyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.36
-
nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.36
-
Pyrazinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.4
-
nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.44
-
methyl nicotinate
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.5
-
Pyrazinamide
recombinant mutant F68W, pH 7.3, 37°C
Oceanobacillus iheyensis
0.55
-
5-methyl nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.56
-
methyl nicotinate
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.57
-
nicotinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.62
-
5-methyl nicotinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.64
-
5-methyl nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.68
-
5-methyl nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.81
-
Pyrazinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.82
-
Pyrazinamide
recombinant mutant E65H, pH 7.3, 37°C
Oceanobacillus iheyensis
0.83
-
Pyrazinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.89
-
methyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
1.02
-
ethyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
1.03
-
methyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
1.11
-
ethyl nicotinate
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
1.15
-
Pyrazinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
1.2
-
Pyrazinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
1.43
-
ethyl nicotinate
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
the enzyme contains 0.235 mM of Zn2+ in 0.242 mM protein, while it only contains 0.0032 mM of Fe2+ and 0.00077 mM Mn2+
Oceanobacillus iheyensis
Zn2+
presence of Zn2+ in the active center of the enzyme in a molecular ratio of 1:1 of protein to metal ion
Oceanobacillus iheyensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21100
-
2 * 21100, mass spectrometry
Oceanobacillus iheyensis
42900
-
gel filtration
Oceanobacillus iheyensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
nicotinamide + H2O
Oceanobacillus iheyensis
-
nicotinate + NH3
-
-
?
nicotinamide + H2O
Oceanobacillus iheyensis HTE831
-
nicotinate + NH3
-
-
?
pyrazinamide + H2O
Oceanobacillus iheyensis
-
pyrazinoic acid + NH3
-
-
?
pyrazinamide + H2O
Oceanobacillus iheyensis HTE831
-
pyrazinoic acid + NH3
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oceanobacillus iheyensis
Q8ESQ6
deep-sea extremely halotolerant and alkaliphilic organism
-
Oceanobacillus iheyensis HTE831
Q8ESQ6
deep-sea extremely halotolerant and alkaliphilic organism
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged wild-type and mutant enzymes methylnicotinamide from Escherichia coli strain Rosetta 2 (DE3) by ultrafiltration and nickel affinity chromatography
Oceanobacillus iheyensis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.06
-
purified recombinant mutant Q96K, pH 7.3, 37°C, substrate ethyl nicotinate
Oceanobacillus iheyensis
0.13
-
purified recombinant wild-type enzyme, pH 7.3, 37°C, substrate ethyl nicotinate
Oceanobacillus iheyensis
0.22
-
purified recombinant mutant Q96A, pH 7.3, 37°C, substrate ethyl nicotinate
Oceanobacillus iheyensis
0.31
-
purified recombinant mutant Q96K, pH 7.3, 37°C, substrate methyl nicotinate
Oceanobacillus iheyensis
0.34
-
purified recombinant mutant C133A, pH 7.3, 37°C, substrate ethyl nicotinate
Oceanobacillus iheyensis
0.36
-
purified recombinant mutant K104A, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
0.37
-
purified recombinant mutant T12Q, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
0.45
-
purified recombinant wild-type enzyme, pH 7.3, 37°C, substrate methyl nicotinate
Oceanobacillus iheyensis
0.46
-
purified recombinant mutant Q96K, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
0.7
-
purified recombinant mutant E65H, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
1.16
-
purified recombinant mutant Q96A, pH 7.3, 37°C, substrate methyl nicotinate
Oceanobacillus iheyensis
1.4
-
purified recombinant mutant C133A, pH 7.3, 37°C, substrate methyl nicotinate
Oceanobacillus iheyensis
1.8
-
purified recombinant mutant Q96A, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
3.62
-
purified recombinant wild-type enzyme, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
3.96
-
purified recombinant mutant Q96K, pH 7.3, 37°C, substrate 5-methyl nicotinamide
Oceanobacillus iheyensis
6.39
-
purified recombinant mutant C133A, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
7.43
-
purified recombinant mutant F68W/C133A, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
7.5
-
purified recombinant mutant F68W, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
8.7
-
purified recombinant mutant Q96K, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
9.01
-
purified recombinant wild-type enzyme, pH 7.3, 37°C, substrate 5-methyl nicotinamide
Oceanobacillus iheyensis
11.74
-
purified recombinant mutant C133A, pH 7.3, 37°C, substrate 5-methyl nicotinamide
Oceanobacillus iheyensis
13.5
-
purified recombinant mutant E65H, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
17.5
-
purified recombinant mutant C133A, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
19.97
-
purified recombinant mutant F68W, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
22.9
-
purified recombinant mutant F68W/C133A, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
23.3
25
purified recombinant wild-type enzyme, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
24.8
-
purified recombinant mutant Q96A, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
28
-
purified recombinant mutant Q96A, pH 7.3, 37°C, substrate 5-methyl nicotinamide
Oceanobacillus iheyensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-methyl nicotinamide + H2O
-
735084
Oceanobacillus iheyensis
5-methyl nicotinate + NH3
-
-
-
?
5-methyl nicotinamide + H2O
-
735084
Oceanobacillus iheyensis HTE831
5-methyl nicotinate + NH3
-
-
-
?
ethyl nicotinate + H2O
-
735084
Oceanobacillus iheyensis
nicotinate + ethanol
-
-
-
?
methyl nicotinate + H2O
-
735084
Oceanobacillus iheyensis
nicotinate + methanol
-
-
-
?
nicotinamide + H2O
-
735084
Oceanobacillus iheyensis
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
preferred substrate
735084
Oceanobacillus iheyensis
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
-
735084
Oceanobacillus iheyensis HTE831
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
preferred substrate
735084
Oceanobacillus iheyensis HTE831
nicotinate + NH3
-
-
-
?
pyrazinamide + H2O
-
735084
Oceanobacillus iheyensis
pyrazinoic acid + NH3
-
-
-
?
pyrazinamide + H2O
residues Cys133 and Phe68 are critical for the pyraminidase activity
735084
Oceanobacillus iheyensis
pyrazinoic acid + NH3
-
-
-
?
pyrazinamide + H2O
-
735084
Oceanobacillus iheyensis HTE831
pyrazinoic acid + NH3
-
-
-
?
pyrazinamide + H2O
residues Cys133 and Phe68 are critical for the pyraminidase activity
735084
Oceanobacillus iheyensis HTE831
pyrazinoic acid + NH3
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 21100, mass spectrometry
Oceanobacillus iheyensis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Oceanobacillus iheyensis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
55
activity range, below 25°C or above 55°C the enzyme loses most of its activity
Oceanobacillus iheyensis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
20
purified recombinant wild-type enzyme, 20 h, stable
Oceanobacillus iheyensis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.05
-
ethyl nicotinate
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.1
-
ethyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.14
-
ethyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.17
-
methyl nicotinate
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.19
-
ethyl nicotinate
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.32
-
methyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.43
-
Pyrazinamide
recombinant mutant E65H, pH 7.3, 37°C
Oceanobacillus iheyensis
0.45
-
Pyrazinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.6
-
methyl nicotinate
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
1.02
-
methyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
1.61
-
Pyrazinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
2.16
-
5-methyl nicotinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
2.37
-
nicotinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
2.6
-
Pyrazinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
3.31
-
Pyrazinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
3.94
-
Pyrazinamide
recombinant mutant F68W, pH 7.3, 37°C
Oceanobacillus iheyensis
5.1
-
5-methyl nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
5.21
-
Pyrazinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
5.63
-
nicotinamide
recombinant mutant E65H, pH 7.3, 37°C
Oceanobacillus iheyensis
7.09
-
5-methyl nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
8.79
-
nicotinamide
recombinant mutant F68W, pH 7.3, 37°C
Oceanobacillus iheyensis
9.82
-
nicotinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
11.65
-
nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
13.6
-
nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
15.9
-
5-methyl nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
16.05
-
nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
-
Oceanobacillus iheyensis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
8
activity range, marked decrease in activity below pH 5.0 and above pH 8.0
Oceanobacillus iheyensis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
7.3
purified recombinant wild-type enzyme, very stable at pH 6.0 and pH 7.3, where it maintains 40% and 30% residual activity, respectively, after 20 hours of incubation
Oceanobacillus iheyensis
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0035
-
nicotinaldehyde
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.0044
-
5-bromonicotinaldehyde
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
Cloned(Commentary) (protein specific)
Commentary
Organism
phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta 2 (DE3)
Oceanobacillus iheyensis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C133A
site-directed mutagenesis, the mutation affectes the active site cavity, and enhances the binding of pyrazinamide compared to the wild-type enzyme
Oceanobacillus iheyensis
E65H
site-directed mutagenesis, the mutation highly reduces the activity with pyrazinamide compared to the wild-type enzyme
Oceanobacillus iheyensis
F68W
site-directed mutagenesis, the mutation affectes the active site cavity, and enhances the binding of pyrazinamide compared to the wild-type enzyme
Oceanobacillus iheyensis
F68W/C133A
site-directed mutagenesis, the mutation affectes the active site cavity, and enhances the binding of pyrazinamide compared to the wild-type enzyme
Oceanobacillus iheyensis
K104A
site-directed mutagenesis, the mutant shows 98.55% reduced activity compared to the wild-type enzyme
Oceanobacillus iheyensis
additional information
mutation of residues Cys133 and Phe68 is required for increasing pyrazinamidase activity 2.9 and 2.5fold, respectively. In addition, the change in the fourth residue involved in the ion metal binding (Glu65) is detrimental to pyrazinamidase activity decreasing it 6fold
Oceanobacillus iheyensis
Q96A
site-directed mutagenesis, the mutant shows similar activity with nicotinamide and increased activity with 5-methyl nicotinamide compared to the wild-type enzyme
Oceanobacillus iheyensis
Q96K
site-directed mutagenesis, the mutant shows 62.7% reduced activity compared to the wild-type enzyme
Oceanobacillus iheyensis
T12Q
site-directed mutagenesis, the mutant shows 98.42% reduced activity compared to the wild-type enzyme
Oceanobacillus iheyensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5-bromonicotinaldehyde
competitive inhibition
Oceanobacillus iheyensis
nicotinaldehyde
competitive inhibition
Oceanobacillus iheyensis
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0035
-
nicotinaldehyde
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.0044
-
5-bromonicotinaldehyde
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Oceanobacillus iheyensis
0.13
-
nicotinamide
recombinant mutant E65H, pH 7.3, 37°C
Oceanobacillus iheyensis
0.17
-
nicotinamide
recombinant mutant F68W, pH 7.3, 37°C
Oceanobacillus iheyensis
0.19
-
nicotinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.26
-
nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.34
-
ethyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.36
-
nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.36
-
Pyrazinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.4
-
nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.44
-
methyl nicotinate
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.5
-
Pyrazinamide
recombinant mutant F68W, pH 7.3, 37°C
Oceanobacillus iheyensis
0.55
-
5-methyl nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.56
-
methyl nicotinate
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.57
-
nicotinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.62
-
5-methyl nicotinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.64
-
5-methyl nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.68
-
5-methyl nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.81
-
Pyrazinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.82
-
Pyrazinamide
recombinant mutant E65H, pH 7.3, 37°C
Oceanobacillus iheyensis
0.83
-
Pyrazinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.89
-
methyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
1.02
-
ethyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
1.03
-
methyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
1.11
-
ethyl nicotinate
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
1.15
-
Pyrazinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
1.2
-
Pyrazinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
1.43
-
ethyl nicotinate
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
the enzyme contains 0.235 mM of Zn2+ in 0.242 mM protein, while it only contains 0.0032 mM of Fe2+ and 0.00077 mM Mn2+
Oceanobacillus iheyensis
Zn2+
presence of Zn2+ in the active center of the enzyme in a molecular ratio of 1:1 of protein to metal ion
Oceanobacillus iheyensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21100
-
2 * 21100, mass spectrometry
Oceanobacillus iheyensis
42900
-
gel filtration
Oceanobacillus iheyensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
nicotinamide + H2O
Oceanobacillus iheyensis
-
nicotinate + NH3
-
-
?
nicotinamide + H2O
Oceanobacillus iheyensis HTE831
-
nicotinate + NH3
-
-
?
pyrazinamide + H2O
Oceanobacillus iheyensis
-
pyrazinoic acid + NH3
-
-
?
pyrazinamide + H2O
Oceanobacillus iheyensis HTE831
-
pyrazinoic acid + NH3
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged wild-type and mutant enzymes methylnicotinamide from Escherichia coli strain Rosetta 2 (DE3) by ultrafiltration and nickel affinity chromatography
Oceanobacillus iheyensis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.06
-
purified recombinant mutant Q96K, pH 7.3, 37°C, substrate ethyl nicotinate
Oceanobacillus iheyensis
0.13
-
purified recombinant wild-type enzyme, pH 7.3, 37°C, substrate ethyl nicotinate
Oceanobacillus iheyensis
0.22
-
purified recombinant mutant Q96A, pH 7.3, 37°C, substrate ethyl nicotinate
Oceanobacillus iheyensis
0.31
-
purified recombinant mutant Q96K, pH 7.3, 37°C, substrate methyl nicotinate
Oceanobacillus iheyensis
0.34
-
purified recombinant mutant C133A, pH 7.3, 37°C, substrate ethyl nicotinate
Oceanobacillus iheyensis
0.36
-
purified recombinant mutant K104A, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
0.37
-
purified recombinant mutant T12Q, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
0.45
-
purified recombinant wild-type enzyme, pH 7.3, 37°C, substrate methyl nicotinate
Oceanobacillus iheyensis
0.46
-
purified recombinant mutant Q96K, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
0.7
-
purified recombinant mutant E65H, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
1.16
-
purified recombinant mutant Q96A, pH 7.3, 37°C, substrate methyl nicotinate
Oceanobacillus iheyensis
1.4
-
purified recombinant mutant C133A, pH 7.3, 37°C, substrate methyl nicotinate
Oceanobacillus iheyensis
1.8
-
purified recombinant mutant Q96A, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
3.62
-
purified recombinant wild-type enzyme, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
3.96
-
purified recombinant mutant Q96K, pH 7.3, 37°C, substrate 5-methyl nicotinamide
Oceanobacillus iheyensis
6.39
-
purified recombinant mutant C133A, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
7.43
-
purified recombinant mutant F68W/C133A, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
7.5
-
purified recombinant mutant F68W, pH 7.3, 37°C, substrate pyrazinamide
Oceanobacillus iheyensis
8.7
-
purified recombinant mutant Q96K, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
9.01
-
purified recombinant wild-type enzyme, pH 7.3, 37°C, substrate 5-methyl nicotinamide
Oceanobacillus iheyensis
11.74
-
purified recombinant mutant C133A, pH 7.3, 37°C, substrate 5-methyl nicotinamide
Oceanobacillus iheyensis
13.5
-
purified recombinant mutant E65H, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
17.5
-
purified recombinant mutant C133A, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
19.97
-
purified recombinant mutant F68W, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
22.9
-
purified recombinant mutant F68W/C133A, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
23.3
25
purified recombinant wild-type enzyme, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
24.8
-
purified recombinant mutant Q96A, pH 7.3, 37°C, substrate nicotinamide
Oceanobacillus iheyensis
28
-
purified recombinant mutant Q96A, pH 7.3, 37°C, substrate 5-methyl nicotinamide
Oceanobacillus iheyensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-methyl nicotinamide + H2O
-
735084
Oceanobacillus iheyensis
5-methyl nicotinate + NH3
-
-
-
?
5-methyl nicotinamide + H2O
-
735084
Oceanobacillus iheyensis HTE831
5-methyl nicotinate + NH3
-
-
-
?
ethyl nicotinate + H2O
-
735084
Oceanobacillus iheyensis
nicotinate + ethanol
-
-
-
?
methyl nicotinate + H2O
-
735084
Oceanobacillus iheyensis
nicotinate + methanol
-
-
-
?
nicotinamide + H2O
-
735084
Oceanobacillus iheyensis
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
preferred substrate
735084
Oceanobacillus iheyensis
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
-
735084
Oceanobacillus iheyensis HTE831
nicotinate + NH3
-
-
-
?
nicotinamide + H2O
preferred substrate
735084
Oceanobacillus iheyensis HTE831
nicotinate + NH3
-
-
-
?
pyrazinamide + H2O
-
735084
Oceanobacillus iheyensis
pyrazinoic acid + NH3
-
-
-
?
pyrazinamide + H2O
residues Cys133 and Phe68 are critical for the pyraminidase activity
735084
Oceanobacillus iheyensis
pyrazinoic acid + NH3
-
-
-
?
pyrazinamide + H2O
-
735084
Oceanobacillus iheyensis HTE831
pyrazinoic acid + NH3
-
-
-
?
pyrazinamide + H2O
residues Cys133 and Phe68 are critical for the pyraminidase activity
735084
Oceanobacillus iheyensis HTE831
pyrazinoic acid + NH3
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 21100, mass spectrometry
Oceanobacillus iheyensis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Oceanobacillus iheyensis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
55
activity range, below 25°C or above 55°C the enzyme loses most of its activity
Oceanobacillus iheyensis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
20
purified recombinant wild-type enzyme, 20 h, stable
Oceanobacillus iheyensis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.05
-
ethyl nicotinate
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.1
-
ethyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.14
-
ethyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.17
-
methyl nicotinate
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.19
-
ethyl nicotinate
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.32
-
methyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.43
-
Pyrazinamide
recombinant mutant E65H, pH 7.3, 37°C
Oceanobacillus iheyensis
0.45
-
Pyrazinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.6
-
methyl nicotinate
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
1.02
-
methyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
1.61
-
Pyrazinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
2.16
-
5-methyl nicotinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
2.37
-
nicotinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
2.6
-
Pyrazinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
3.31
-
Pyrazinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
3.94
-
Pyrazinamide
recombinant mutant F68W, pH 7.3, 37°C
Oceanobacillus iheyensis
5.1
-
5-methyl nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
5.21
-
Pyrazinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
5.63
-
nicotinamide
recombinant mutant E65H, pH 7.3, 37°C
Oceanobacillus iheyensis
7.09
-
5-methyl nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
8.79
-
nicotinamide
recombinant mutant F68W, pH 7.3, 37°C
Oceanobacillus iheyensis
9.82
-
nicotinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
11.65
-
nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
13.6
-
nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
15.9
-
5-methyl nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
16.05
-
nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
-
Oceanobacillus iheyensis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
8
activity range, marked decrease in activity below pH 5.0 and above pH 8.0
Oceanobacillus iheyensis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
7.3
purified recombinant wild-type enzyme, very stable at pH 6.0 and pH 7.3, where it maintains 40% and 30% residual activity, respectively, after 20 hours of incubation
Oceanobacillus iheyensis
General Information
General Information
Commentary
Organism
metabolism
nicotinamidases catalyze the hydrolysis of nicotinamide to nicotinic acid and ammonia, an important reaction in the NAD+ salvage pathway
Oceanobacillus iheyensis
additional information
two for residues involved in the catalysis (K104 and Q96), one for the fourth residue involved in metal binding (E65), one for the residue forming hydrogen bonds between the main and lateral chains (T12), one for the residue involved in the cis-peptide bond and in the formation of the oxyanion hole (C133), and one for the residue forming one of the faces of the active site F68
Oceanobacillus iheyensis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
nicotinamidases catalyze the hydrolysis of nicotinamide to nicotinic acid and ammonia, an important reaction in the NAD+ salvage pathway
Oceanobacillus iheyensis
additional information
two for residues involved in the catalysis (K104 and Q96), one for the fourth residue involved in metal binding (E65), one for the residue forming hydrogen bonds between the main and lateral chains (T12), one for the residue involved in the cis-peptide bond and in the formation of the oxyanion hole (C133), and one for the residue forming one of the faces of the active site F68
Oceanobacillus iheyensis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.05
-
ethyl nicotinate
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.1
-
ethyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.13
-
ethyl nicotinate
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.31
-
methyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.38
-
methyl nicotinate
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.39
-
Pyrazinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.41
-
ethyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.52
-
Pyrazinamide
recombinant mutant E65H, pH 7.3, 37°C
Oceanobacillus iheyensis
1.14
-
methyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
1.17
-
methyl nicotinate
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
1.34
-
Pyrazinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
3.2
-
Pyrazinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
3.4
-
5-methyl nicotinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
4.15
-
nicotinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
6.28
-
Pyrazinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
7.5
-
5-methyl nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
7.9
-
Pyrazinamide
recombinant mutant F68W, pH 7.3, 37°C
Oceanobacillus iheyensis
9.19
-
Pyrazinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
11.07
-
5-methyl nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
29.04
-
5-methyl nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
34
-
nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
42.65
-
nicotinamide
recombinant mutant E65H, pH 7.3, 37°C
Oceanobacillus iheyensis
43.48
-
nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
44.59
-
nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
52.01
-
nicotinamide
recombinant mutant F68W, pH 7.3, 37°C
Oceanobacillus iheyensis
52.8
-
nicotinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.05
-
ethyl nicotinate
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.1
-
ethyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.13
-
ethyl nicotinate
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.31
-
methyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
0.38
-
methyl nicotinate
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.39
-
Pyrazinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
0.41
-
ethyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
0.52
-
Pyrazinamide
recombinant mutant E65H, pH 7.3, 37°C
Oceanobacillus iheyensis
1.14
-
methyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
1.17
-
methyl nicotinate
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
1.34
-
Pyrazinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
3.2
-
Pyrazinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
3.4
-
5-methyl nicotinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
4.15
-
nicotinamide
recombinant mutant Q96K, pH 7.3, 37°C
Oceanobacillus iheyensis
6.28
-
Pyrazinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
7.5
-
5-methyl nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
7.9
-
Pyrazinamide
recombinant mutant F68W, pH 7.3, 37°C
Oceanobacillus iheyensis
9.19
-
Pyrazinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
11.07
-
5-methyl nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
29.04
-
5-methyl nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
34
-
nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
42.65
-
nicotinamide
recombinant mutant E65H, pH 7.3, 37°C
Oceanobacillus iheyensis
43.48
-
nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
Oceanobacillus iheyensis
44.59
-
nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
Oceanobacillus iheyensis
52.01
-
nicotinamide
recombinant mutant F68W, pH 7.3, 37°C
Oceanobacillus iheyensis
52.8
-
nicotinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
Oceanobacillus iheyensis
Other publictions for EC 3.5.1.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727053
Stekhanova
Nicotinamidase from the thermo ...
Acidilobus saccharovorans, Acidilobus saccharovorans DSM 16705
Biochemistry
79
54-61
2014
2
-
1
-
-
-
4
1
-
1
1
-
-
7
-
-
-
-
-
-
-
-
2
1
1
1
2
1
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
4
-
1
-
1
1
-
-
-
-
-
-
-
-
-
2
1
1
1
2
1
1
-
-
-
-
1
1
-
1
1
729184
Stekhanova
Nicotinamidase from the thermo ...
Acidilobus saccharovorans, Acidilobus saccharovorans DSM 16705
Biochemistry (Moscow)
79
54-61
2014
3
-
1
-
-
-
3
2
-
1
1
-
-
7
-
-
-
-
-
-
-
-
6
1
1
1
2
2
1
1
-
-
-
-
-
3
-
1
-
-
-
-
-
3
-
2
-
1
1
-
-
-
-
-
-
-
-
-
6
1
1
1
2
2
1
1
-
-
-
-
-
-
2
2
734741
Ion
A multi-scale computational st ...
Streptococcus pneumoniae
Molecules
19
15735-15753
2014
-
1
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
735324
Rueda
Nicotinamidase/pyrazinamidase ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Tuberculosis
94
644-648
2014
-
-
1
-
-
-
-
-
-
-
1
4
-
3
-
-
1
-
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
1
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
735084
Sanchez-Carron
Biochemical and mutational ana ...
Oceanobacillus iheyensis, Oceanobacillus iheyensis HTE831
PLoS ONE
8
e56727
2013
-
-
1
-
9
-
2
27
-
2
2
4
-
5
-
-
1
-
-
-
28
-
12
1
1
1
1
26
1
1
1
-
2
-
-
-
-
1
-
-
9
-
-
2
2
27
-
2
2
4
-
-
-
1
-
-
28
-
12
1
1
1
1
26
1
1
1
-
-
2
2
-
26
26
718924
Smith
Structural and kinetic isotope ...
Saccharomyces cerevisiae
Biochemistry
51
243-256
2012
-
-
1
1
10
-
6
8
-
1
-
1
-
6
-
-
1
-
-
-
-
-
7
-
-
-
-
12
1
-
-
-
5
-
-
-
-
1
-
1
10
-
-
6
5
8
-
1
-
1
-
-
-
1
-
-
-
-
7
-
-
-
-
12
1
-
-
-
-
-
-
-
15
15
719619
Gazanion
Leishmania infantum nicotinami ...
Leishmania infantum
Int. J. Parasitol.
42
323-327
2012
-
-
-
-
1
-
-
-
-
-
-
1
-
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
735054
Zhang
Molecular dynamics simulations ...
Acinetobacter baumannii, Streptococcus pneumoniae
PLoS ONE
7
e39546
2012
-
-
-
2
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
720499
Gazanion
The Leishmania nicotinamidase ...
Leishmania infantum, Leishmania infantum MHOM/MA/67/ITMAP-263
Mol. Microbiol.
82
21-38
2011
-
-
1
1
1
-
-
-
1
-
2
4
-
7
-
-
1
-
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
1
-
2
4
-
-
-
1
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
718859
French
Characterization of nicotinami ...
Borreliella burgdorferi, Caenorhabditis elegans, Plasmodium falciparum, Saccharomyces cerevisiae, Streptococcus pneumoniae
Biochemistry
49
10421-10439
2010
-
-
5
-
4
-
46
6
-
-
-
45
-
17
-
-
5
-
-
-
-
-
45
-
-
-
-
6
-
-
-
-
32
-
-
-
-
5
-
-
4
-
-
46
32
6
-
-
-
45
-
-
-
5
-
-
-
-
45
-
-
-
-
6
-
-
-
-
-
5
5
-
-
-
718883
French
High-resolution crystal struct ...
Streptococcus pneumoniae
Biochemistry
49
8803-8812
2010
-
-
1
1
1
-
2
-
-
5
1
1
-
5
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
2
-
-
-
5
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718885
Seiner
Kinetics and inhibition of nic ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biochemistry
49
9613-9619
2010
-
-
1
-
1
-
4
4
-
2
-
4
-
162
-
-
1
-
-
-
-
-
4
-
-
-
-
4
1
-
-
-
2
-
-
-
-
1
-
-
1
-
-
4
2
4
-
2
-
4
-
-
-
1
-
-
-
-
4
-
-
-
-
4
1
-
-
-
-
1
1
-
4
4
710862
Fyfe
Specificity and mechanism of A ...
Acinetobacter baumannii
Angew. Chem. Int. Ed. Engl.
48
9176-9179
2009
-
-
-
-
-
-
-
-
-
4
1
2
-
2
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
2
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
711827
Vrablik
Nicotinamidase modulation of N ...
Caenorhabditis elegans
Development
136
3637-3646
2009
-
-
1
-
1
-
-
-
1
1
-
1
-
4
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
679806
Zhang
Characterization of Mycobacter ...
Mycobacterium tuberculosis
FEBS J.
275
753-762
2008
-
1
1
-
9
-
-
-
-
2
1
2
-
3
-
-
1
-
-
-
2
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
1
1
-
-
9
-
-
-
-
-
-
2
1
2
-
-
-
1
-
-
2
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
697513
Chong
Enhanced tolerance against ear ...
Drosophila melanogaster
Curr. Neurovasc. Res.
5
159-170
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698121
McClure
Pnc1p-mediated nicotinamide cl ...
Saccharomyces cerevisiae
Genetics
180
797-810
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698815
Balan
Life span extension and neuron ...
Drosophila melanogaster
J. Biol. Chem.
283
27810-27819
2008
-
-
1
-
-
-
-
-
-
-
1
-
-
6
-
-
1
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700669
Yin
Changes in pyridine metabolism ...
Lotus japonicus
Phytochemistry
69
2891-2898
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677824
Hu
Crystal structure of the yeast ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
461
66-75
2007
-
-
1
1
-
-
-
1
-
1
-
2
-
3
-
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681746
van der Horst
The Caenorhabditis elegans nic ...
Caenorhabditis elegans
Mech. Ageing Dev.
128
346-349
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682376
Wang
Nicotinamidase participates in ...
Arabidopsis thaliana
Plant J.
49
1020-1029
2007
-
-
-
-
-
-
-
1
-
-
-
1
-
6
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
682380
Hunt
Nicotinamidase activity is imp ...
Arabidopsis thaliana
Plant J.
51
341-351
2007
2
-
1
-
-
-
3
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659608
Zheng
Changes in trigonelline (N-met ...
Vigna radiata var. radiata
J. Exp. Bot.
56
1615-1623
2005
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
3
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
668655
Kim
Brucella abortus nicotinamidas ...
Brucella abortus, Brucella abortus 544 / ATCC 23448
FEMS Microbiol. Lett.
234
289-295
2004
-
-
-
-
1
-
-
-
2
-
-
2
-
8
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670237
Gallo
Nicotinamide clearance by Pnc1 ...
Saccharomyces cerevisiae
Mol. Cell. Biol.
24
1301-1312
2004
1
-
1
-
2
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246584
Guo
Mycobacterium smegmatis has tw ...
Mycolicibacterium smegmatis
J. Bacteriol.
182
3881-3884
2000
-
-
1
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246583
Sun
Reduced pyrazinamidase activit ...
Mycobacterium kansasii
Antimicrob. Agents Chemother.
43
537-542
1999
-
-
-
-
-
-
1
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246585
Boshoff
Purification, gene cloning, ta ...
Mycobacterium tuberculosis, Mycolicibacterium smegmatis
J. Bacteriol.
180
5809-5814
1998
-
-
1
-
-
-
-
2
-
-
-
-
-
5
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246587
Raynaud
-
Extracellular enzyme activitie ...
Mycobacterium kansasii, Mycobacterium tuberculosis, Mycobacterium tuberculosis variant bovis, Mycolicibacterium fortuitum, Mycolicibacterium phlei
Microbiology
144
577-587
1998
-
-
-
-
-
-
-
-
6
-
-
-
-
5
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246582
Frothingham
Identification, cloning, and e ...
Escherichia coli
Antimicrob. Agents Chemother.
40
1426-1431
1996
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246586
Zrez
Increased nicotinamideadenine ...
Plasmodium falciparum
Blood
75
1705-1710
1990
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246588
Kgayama
-
Purification and properties of ...
Pseudomonas sp., Pseudomonas sp. GDI 211
Agric. Biol. Chem.
54
2565-2571
1990
-
-
-
-
-
1
2
2
-
3
2
2
-
2
-
-
1
-
-
-
1
-
6
1
1
1
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
2
-
3
2
2
-
-
-
1
-
-
1
-
6
1
1
1
2
-
1
1
-
-
-
-
-
-
-
-
246581
Yan
Purification and characterizat ...
Saccharomyces cerevisiae
J. Biol. Chem.
262
9082-9087
1987
-
-
-
-
-
-
2
1
-
-
2
-
-
2
-
-
1
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
2
-
-
-
-
1
-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1627
Shibata
-
Tissue distribution of the enz ...
Rattus norvegicus
Agric. Biol. Chem.
50
3037-3041
1986
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209135
Foster
NAD metabolism in Vibrio chole ...
Vibrio cholerae
J. Bacteriol.
149
368-371
1982
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246576
Wintzerith
Purification and characterizat ...
Mus musculus
Biochim. Biophys. Acta
613
191-202
1980
1
-
-
-
-
1
13
2
-
3
3
1
-
1
-
1
1
-
-
1
-
1
2
1
1
-
2
-
3
-
-
-
-
-
-
1
-
-
-
-
-
1
-
13
-
2
-
3
3
1
-
-
1
1
-
1
-
1
2
1
1
-
2
-
3
-
-
-
-
-
-
-
-
-
246577
Fuller
-
Determination of nicotinamide ...
Saccharomyces cerevisiae
Methods Enzymol.
66
3-4
1980
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246578
Tanigawa
Nicotinamide deamidase from Fl ...
Flavobacterium peregrinum
Methods Enzymol.
66
132-136
1980
-
-
-
-
-
1
11
1
-
-
1
-
-
1
-
-
1
-
-
-
1
1
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
11
-
1
-
-
1
-
-
-
-
1
-
-
1
1
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
246575
Foster
Pyridine nucleotide cycle of S ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
138
957-961
1979
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246573
Gadd
-
Kinetic studies of nicotinamid ...
Micrococcus luteus
Int. J. Biochem.
5
397-407
1974
-
-
-
-
-
-
3
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
246574
Johnson
-
Inhibition of nicotinamide dea ...
Micrococcus luteus
Int. J. Biochem.
5
633-641
1974
-
-
-
-
-
-
19
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246572
Gillam
Nicotinamide deamidase from ra ...
Oryctolagus cuniculus
Arch. Biochem. Biophys.
157
268-284
1973
-
-
-
-
-
-
5
-
-
-
2
-
-
1
1
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
2
-
-
1
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246570
Albizati
Active-site studies on rabbit ...
Oryctolagus cuniculus
Biochemistry
11
1508-1516
1972
-
-
-
-
-
-
7
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
1
-
-
-
-
-
-
1
-
-
2
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
246571
Tanigawa
Purification and properties of ...
Flavobacterium peregrinum
J. Biol. Chem.
247
8036-8042
1972
1
-
-
-
-
1
6
1
-
-
1
-
-
1
-
-
1
-
-
-
2
1
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
6
-
1
-
-
1
-
-
-
-
1
-
-
2
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
246569
Pardee
Hyperproduction and purificati ...
Escherichia coli
J. Biol. Chem.
246
6792-6796
1971
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
1
1
1
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
1
1
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
246568
Petrack
Nicotinamide deaminase from ma ...
Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
240
1725-1730
1965
-
-
-
-
-
-
-
1
3
-
-
-
-
2
-
-
2
-
-
2
2
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
2
-
2
2
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
246567
Sarma
-
Studies on the enzymes involve ...
Aspergillus niger
Biochim. Biophys. Acta
81
311-322
1964
-
-
-
-
-
-
4
1
-
1
-
-
-
1
-
-
1
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
1
-
-
-
-
-
1
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-