EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
2.4.1.20 | 0.00048 |
- |
lactose |
pH 6.6, 37°C, recombinant wild-type enzyme |
706588 |
2.4.1.20 | 0.0015 |
- |
lactose |
pH 6.6, 37°C, recombinant mutant T508I/N667A |
706588 |
2.4.1.20 | 0.0021 |
- |
lactose |
pH 6.6, 37°C, recombinant mutant T508A/N667T |
706588 |
2.4.1.20 | 0.0034 |
- |
lactose |
pH 6.6, 37°C, recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S |
706588 |
2.4.1.20 | 0.006 |
- |
methyl alpha-D-glucoside |
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C |
719136 |
2.4.1.20 | 0.0198 |
- |
2-deoxy-D-glucose |
mutant enzyme C485A, at pH 6.0 and 37°C |
735893 |
2.4.1.20 | 0.0263 |
- |
D-mannose |
mutant enzyme Y648V, at pH 6.0 and 37°C |
735893 |
2.4.1.20 | 0.031 |
- |
D-glucose |
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C |
719136 |
2.4.1.20 | 0.033 |
- |
ethyl beta-D-glucoside |
mutant T508I/E649C/N667A, pH 6.6, 37°C |
719136 |
2.4.1.20 | 0.0376 |
- |
N-acetyl-D-glucosamine |
mutant enzyme Y648V, at pH 6.0 and 37°C |
735893 |