EC Number   |
General Information |
Reference |
|---|
   7.5.2.6 | evolution |
enzyme MsbA encoded is a member of the ABC transporter family |
751020 |
| 7.5.2.6 | evolution |
Pseudomonas aeruginosa MsbA encoded is a member of the ABC transporter family, but this protein has distinctive features when compared with other MsbA proteins |
-, 751020 |
| 7.5.2.6 | evolution |
the msbA gene product belongs to the superfamily of ABC transporters, the ATP-binding cassette (ABC)-transporter superfamily, a universally conserved family of proteins characterized by a highly conserved ATP-binding domain |
751587 |
| 7.5.2.6 | evolution |
the MsbA protein is an essential ABC (ATP-binding-cassette) superfamily member in Gram-negative bacteria |
749842 |
| 7.5.2.6 | malfunction |
accumulation of lipid A species in the inner membranes of htrB- cells when msbA and orfE are not expressed. The tetra-acylated lipid A precursors that accumulate in htrB mutants may not be transported as efficiently by MsbA as are penta- or hexaacylated lipid A species. LPS accumulates in the inner membranes of htrB-deficient mutants. Introduction of msbA and orfE on low copy plasmids partially restores translocation of LPS to the outer membrane at 42°C in htrB mutants. The Escherichia coli msbA gene also functions as a multicopy suppressor of htrB mutations |
-, 751007 |
| 7.5.2.6 | malfunction |
depletion or loss of function of MsbA results in the accumulation of lipopolysaccharide and phospholipids in the inner membrane of Escherichia coli |
-, 751011 |
| 7.5.2.6 | malfunction |
msbA is an essential gene in this organism and mutation in this gene is lethal to the bacterium. Disruption of the chromosomal msbA is achieved only when a functional copy of the gene is provided in trans. Gene msbA from Escherichia coli (msbAEc) cannot cross complement the msbA merodiploid cells of Pseuomonas aeruginosa |
-, 751020 |
| 7.5.2.6 | metabolism |
the enzyme is involved in the biosynthetic pathways of lipid A |
-, 751020 |
| 7.5.2.6 | metabolism |
the enzyme is involved in the lipid A biosynthesis. MsbA cannot efficiently transport a substrate lacking phosphorylation at the 4'-position of lipid A. This last essential step of lipid A biosynthesis is catalysed by LpxK, the two proteins are concatenated in some bacteria, suggesting tight coupling of their cellular activities |
751693 |
| 7.5.2.6 | more |
inward facing structure of EcMsbA in complex with LPS, structure comparisons and molecular basis of active lipid transport, overview |
751693 |
