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Literature summary for 7.5.2.6 extracted from

  • Polissi, A.; Georgopoulos, C.
    Mutational analysis and properties of the msbA gene of Escherichia coli, coding for an essential ABC family transporter (1996), Mol. Microbiol., 20, 1221-1233 .
    View publication on PubMed
Search for more literature for 7.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
gene msbA, recombinant expression in Escherichia coli strain BL21 inner membranes. At 42°C and in the absence of transducer arabinose, the msbA gene is transcribed poorly, if at all Escherichia coli

Protein Variants

Protein Variants Comment Organism
D510G random mutagenesis, the mutant cannot support Escherichia coli growth, but it retains the ability to bind ATP in vitro Escherichia coli
I517V random mutagenesis, the MsbA mutant protein is still partly functional due to the fact that an Ile to Val change is a fairly conservative substitution, or because in the MDR proteins a Val residue is present at this position Escherichia coli
L509P random mutagenesis, the mutant cannot support Escherichia coli growth, but it retains the ability to bind ATP in vitro Escherichia coli
additional information random PCR mutagenesis of gene msbA resulting in six independent mutants, four of which result in single-amino-acid substitutions in non-conserved residues, the temperature-sensitive mutants are able to support cell growth at 30°C but not at 43°C. The remaining two mutants behave as recessive lethals, the mutations result in single-amino-acid substitutions in Walker motif B, one of the two highly conserved regions of the ATP-binding domain. The latter two mutants cannot support Escherichia coli growth, but they both retain the ability to bind ATP in vitro. N-acetyl [3H]-glucosamine, a precursor of Iipopolysaccharides, accumulates at the non-permissive temperature in the inner membrane of either htrB null or msbA conditional lethal strains. Translocation of the precursor to the outer membrane is restored by transformation with a plasmid containing the wild-type msbA gene. The Ts- phenotype exhibited at 43°C can be suppressed by supplementing the medium with 10 mM Mg2+ or Ca2+ Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
inner membrane an integral membrane protein Escherichia coli
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-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + lipid A-core oligosaccharide[side 1] Escherichia coli
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 ADP + phosphate + lipid A-core oligosaccharide[side 2]
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P60752
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + lipid A-core oligosaccharide[side 1]
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 Escherichia coli ADP + phosphate + lipid A-core oligosaccharide[side 2]
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 ?

Synonyms

Synonyms Comment Organism
MsbA
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 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

General Information

General Information Comment Organism
evolution the msbA gene product belongs to the superfamily of ABC transporters, the ATP-binding cassette (ABC)-transporter superfamily, a universally conserved family of proteins characterized by a highly conserved ATP-binding domain Escherichia coli
physiological function the msbA gene expressed from a low-copy-number plasmid vector is able to suppress the temperature-sensitive growth phenotype of an Escherichia coli htrB null mutant as well as the accumulation of phospholipids. The msbA gene is essential for bacterial viability at all temperatures. Role for MsbA as a translocator of lipopolysaccharides or its precursors Escherichia coli