EC Number   |
General Information |
Reference |
|---|
   3.4.17.10 | evolution |
the amino acid sequence of carboxypeptidase E appears to be highly conserved among chicken, mouse, and human. The C-terminal sequence of carboxypeptidase E, which serves as an anchor to the membrane of secretory granules, is perfectly conserved between birds and mammals |
708044 |
| 3.4.17.10 | malfunction |
cpe -/- mice is used as a model of EEC dysfunction. Using this model it is shown that CPE exhibits a relevant for EEC function and intestinal homeostasis |
732761 |
| 3.4.17.10 | malfunction |
in Neuro 2a cells that lack CPE, PC1/3 and PC2, proglucagon co-localise with the Golgi marker p115 as determined by quantitative immunofluorescence microscopy. siRNA-mediated knockdown of CPE disrupts regulated secretion of glucagon from pancreatic-derived alphaTC1-6 cells, but not of GLP-1 from intestinal cell-derived GLUTag cells |
732239 |
| 3.4.17.10 | malfunction |
in two types of carboxypeptidase E mutant mice, Cpefat/Cpefat and Cpe knockout, loss of normal carboxypeptidase E leads to a lot of disorders, including diabetes, hyperproinsulinemia, low bone mineral density and deficits in learning and memory |
753432 |
| 3.4.17.10 | physiological function |
carboxypeptidase E is classically known as an exopeptidase that cleaves carboxy-terminal basic aminoacids from neuropeptide and peptide hormone intermediates resulting in the production of bioactive peptides inneuroendocrine cells. Carboxypeptidase E that is expressed in the nervous and endocrine systems plays multiple non-enzymatic roles in addition to being an exopeptidase The soluble form of carboxypeptidase E acts as a processing enzyme. The membrane-bound form of carboxypeptidase E serves as a sorting receptor for some proneuropeptides and pro-brain derived neurotrophic factor to target them into the regulated secretory pathway.The cytoplasmic tail of the transmembrane form of carboxypeptidase E is shown to be a key molecule in the anchoring of adrenocorticotropic hormone and brain derived neurotrophic factor vesicles to the microtubule-based transport system for post-Golgi delivery of the vesicles to the release site |
709587 |
| 3.4.17.10 | physiological function |
carboxypeptidase E mediates the effects of nitric oxide synthase 1 adaptor protein on dendrite morphology |
709639 |
| 3.4.17.10 | physiological function |
carboxypeptidase E plays a role in regulating proper dendritic patterning, especially dendritic pruning and spine formation that are necessary for appropriate synaptogenesis and the establishment of neuronal network |
709627 |
| 3.4.17.10 | physiological function |
carboxypeptidase E, a prohormone processing exopeptidase and sorting receptor for the regulated secretory pathway, interacts with the dopamine transporter carboxyl terminus and affects dopamine transporter function. Mammalian cell lines coexpressing carboxypeptidase E and dopamine transporter exhibit increased dopamine transporter-mediated dopamine uptake activity compared to cells expressing dopamine transporter alone. Moreover, coexpression of an interfering dopamine transporter-carboxyl terminus minigene inhibit the effects of carboxypeptidase E on dopamine transporter. Functional changes caused by carboxypeptidase E could be attributed to enhanced dopamine transporter expression and subsequent increase in dopamine transporter cell surface localization, due to decreased dopamine transporter degradation. In addition, carboxypeptidase E association could reduce the phosphorylation state of dopamine transporter on serine residues, potentially leading to reduced internalization, thus stabilizing plasmalemmal dopamine transporter localization |
709901 |
| 3.4.17.10 | physiological function |
CPE acts extracellularly through activation of ERK and AKT signaling pathways to up-regulate expression of the anti-apoptotic protein BCL-2 to mediate neuroprotection of neurons during stress |
732743 |
| 3.4.17.10 | physiological function |
CPE can mediate neuroprotection of the hippocampal neurons during mild chronic restraint stress and may play an important role in maintaining allostasis |
731720 |
