EC Number |
General Information |
Reference |
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2.7.1.145 | evolution |
evolution of dNKs, overview. The two families of dNKs, the TK1-like kinases and the non-TK1-like kinases, have overall unique monomer structures and only share significant protein sequence similarity at the Ploop, a glycine rich ATP binding domain close to the N-terminus. This suggests that the two families have different evolutionary origins. The insect dNK is a non-TK1 like kinase |
738781 |
2.7.1.145 | more |
enzyme identification using a mutagenic nucleoside analogue 2-hydroxy-2'-deoxyadenosine 5'-monophosphate, overview. Method development and evaluation for in vivo evolution selection method for active deoxyribonucleoside kinase proteins, including selection of antibiotic-resistant colonies resulting frommutations by phosphorylated 2-hydroxy-deoxyadenosine and the subsequent isolation of the plasmid DNAs. Fifteen Dm-dNK mutants selected after the seventh and eighth evolutioncycles are actually active in vivo, one shows activity similar to the wild-typ enzyme |
738739 |
2.7.1.145 | more |
structure of non-TK1 like kinases, overview |
738781 |
2.7.1.145 | physiological function |
DmdNK is cell cycle regulated and under the control of cyclin E and the dE2F1 transcription factor, involved in G1/S phase transition |
738781 |
2.7.1.145 | physiological function |
dNK expression increases substantially sensitivity of cells to 5-aza-2'-deoxycytidine and 2'-deoxy-zebularine |
703396 |
2.7.1.145 | physiological function |
no differences between mutant cytosolic Dm-dNK, mitochondrial Dm-dNK, and wild-type nuclear Dm-dNK in regards to enzyme activity, cellular sensitivity to nucleoside analogues, or bystander cell killing. Tan IIA may influence the bystander effect of cancer cells expressing Dm-dNK by regulating the expression of Cx43 and Cx26. Tanshinone (Tan) IIA is a fat-soluble and pharmacologically active ingredient of Danshen, a traditional Chinese medicine used in the treatment of cardiovascular diseases. It is isolated from the rhizome of a Chinese herb Salvia miltiorrhiza. Tan IIA treatment of osteosarcoma cells results in the upregulation of Cx43 and Cx26 proteins |
739211 |
2.7.1.145 | physiological function |
the enzyme is involved in chloroplast development |
762135 |