EC Number |
Reference |
---|
3.5.1.87 | - |
288949, 711551 |
3.5.1.87 | a one-step purification procedure of the recombinant L-N-carbamoylase fused to the HIS6-tag is employed using immobilized cobalt affinity chromatography |
675499 |
3.5.1.87 | ammonium sulfate, phenyl-Sepharose CL-4B, partially purified |
209293 |
3.5.1.87 | ammonium sulfate, phenyl-Sepharose, DEAE-cellulose, phenyl-Sepharose, Sephadex G-100 |
288946 |
3.5.1.87 | anion-exchange chromatography, hydrophobic interaction chromatography hydroxyapatite chromatography used for purification of native expressed protein |
671503 |
3.5.1.87 | DEAE-Sephacel, ammonium sulfate, phenyl-Sepharose CL-4B, Mono Q, Superose 12 HR |
288945 |
3.5.1.87 | native enzyme 60fold to homogeneity by heat treatment at 70°C for 2 min, anion exchange chromatography of the supernatant, hydrophobic interaction chromatography, ultrafiltration, and gel filtration |
733543, 733544 |
3.5.1.87 | one-step purification procedure of SmLcar mutants fused to the His6 tag employing cobalt affinity chromatography |
672447 |
3.5.1.87 | purified 58fold to homogeneity with a yield of 16.1% by three steps of column chromatography |
707251 |
3.5.1.87 | purified to over 95% purity |
690255 |