BRENDA - Enzyme Database show
show all sequences of 3.5.1.87

N-carbamyl-L-amino acid amidohydrolase of Pseudomonas sp. strain NS671: purification and some properties of the enzyme expressed in Escherichia coli

Ishikawa, T.; Watabe, K.; Mukohara, Y.; Nakamura, H.; Biosci. Biotechnol. Biochem. 60, 612-615 (1996)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
ATP
0.5 mM, approximately 50% inhibition, 1.5 mM, approximately 85% inhibition
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
-
Pseudomonas sp.
Mn2+
-
Pseudomonas sp.
Ni2+
-
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE
Pseudomonas sp.
109000
-
recombinant enzyme expressed in E. coli, gel filtration
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. NS671
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, phenyl-Sepharose, DEAE-cellulose, phenyl-Sepharose, Sephadex G-100
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
21.5
-
at pH 7.5 and 42°C
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-carbamoyl-DL-alanine + H2O
-
288946
Pseudomonas sp.
L-alanine + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-DL-alanine + H2O
-
288946
Pseudomonas sp. NS671
L-alanine + CO2 + NH3
-
288946
Pseudomonas sp. NS671
?
N-carbamoyl-DL-methionine + H2O
-
288946
Pseudomonas sp.
2-aminohexanoic acid + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-DL-phenylalanine + H2O
-
288946
Pseudomonas sp.
L-phenylalanine + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-DL-valine + H2O
-
288946
Pseudomonas sp.
L-valine + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-DL-valine + H2O
-
288946
Pseudomonas sp. NS671
L-valine + CO2 + NH3
-
288946
Pseudomonas sp. NS671
?
N-carbamoyl-L-isoleucine + H2O
-
288946
Pseudomonas sp.
L-isoleucine + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-L-isoleucine + H2O
-
288946
Pseudomonas sp. NS671
L-isoleucine + CO2 + NH3
-
288946
Pseudomonas sp. NS671
?
N-carbamoyl-L-leucine + H2O
-
288946
Pseudomonas sp.
L-leucine + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-L-leucine + H2O
-
288946
Pseudomonas sp. NS671
L-leucine + CO2 + NH3
-
288946
Pseudomonas sp. NS671
?
N-carbamoyl-L-methionine + H2O
L-stereospecific enzyme
288946
Pseudomonas sp.
L-methionine + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-L-methionine + H2O
L-stereospecific enzyme
288946
Pseudomonas sp. NS671
L-methionine + CO2 + NH3
-
288946
Pseudomonas sp. NS671
?
N-carbamoyl-L-tyrosine + H2O
-
288946
Pseudomonas sp.
L-tyrosine + CO2 + NH3
-
288946
Pseudomonas sp.
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 45000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Pseudomonas sp.
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
50
-
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
activity is lost after 60 min at 25°C
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Pseudomonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
9
-
Pseudomonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ATP
0.5 mM, approximately 50% inhibition, 1.5 mM, approximately 85% inhibition
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
-
Pseudomonas sp.
Mn2+
-
Pseudomonas sp.
Ni2+
-
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE
Pseudomonas sp.
109000
-
recombinant enzyme expressed in E. coli, gel filtration
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, phenyl-Sepharose, DEAE-cellulose, phenyl-Sepharose, Sephadex G-100
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
21.5
-
at pH 7.5 and 42°C
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-carbamoyl-DL-alanine + H2O
-
288946
Pseudomonas sp.
L-alanine + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-DL-alanine + H2O
-
288946
Pseudomonas sp. NS671
L-alanine + CO2 + NH3
-
288946
Pseudomonas sp. NS671
?
N-carbamoyl-DL-methionine + H2O
-
288946
Pseudomonas sp.
2-aminohexanoic acid + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-DL-phenylalanine + H2O
-
288946
Pseudomonas sp.
L-phenylalanine + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-DL-valine + H2O
-
288946
Pseudomonas sp.
L-valine + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-DL-valine + H2O
-
288946
Pseudomonas sp. NS671
L-valine + CO2 + NH3
-
288946
Pseudomonas sp. NS671
?
N-carbamoyl-L-isoleucine + H2O
-
288946
Pseudomonas sp.
L-isoleucine + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-L-isoleucine + H2O
-
288946
Pseudomonas sp. NS671
L-isoleucine + CO2 + NH3
-
288946
Pseudomonas sp. NS671
?
N-carbamoyl-L-leucine + H2O
-
288946
Pseudomonas sp.
L-leucine + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-L-leucine + H2O
-
288946
Pseudomonas sp. NS671
L-leucine + CO2 + NH3
-
288946
Pseudomonas sp. NS671
?
N-carbamoyl-L-methionine + H2O
L-stereospecific enzyme
288946
Pseudomonas sp.
L-methionine + CO2 + NH3
-
288946
Pseudomonas sp.
?
N-carbamoyl-L-methionine + H2O
L-stereospecific enzyme
288946
Pseudomonas sp. NS671
L-methionine + CO2 + NH3
-
288946
Pseudomonas sp. NS671
?
N-carbamoyl-L-tyrosine + H2O
-
288946
Pseudomonas sp.
L-tyrosine + CO2 + NH3
-
288946
Pseudomonas sp.
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 45000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Pseudomonas sp.
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
50
-
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
activity is lost after 60 min at 25°C
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Pseudomonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
9
-
Pseudomonas sp.
Other publictions for EC 3.5.1.87
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733195
Soriano-Maldonado
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-
2
1
-
1
-
-
-
-
1
-
-
-
5
-
-
1
-
-
-
-
-
21
-
1
1
1
-
1
-
-
-
-
-
-
-
2
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
21
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
735201
Soriano-Maldonado
-
Amidohydrolase process: expand ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus CECT43
Process Biochem.
49
1281-1287
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-
1
1
-
-
-
-
-
-
1
-
4
-
3
-
-
-
-
-
-
-
-
19
-
1
-
1
-
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
19
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
733543
Nandanwar
Trimeric l-N-carbamoylase from ...
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60
219-230
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-
1
-
-
-
-
7
1
-
3
2
1
-
4
-
-
1
-
-
-
1
-
13
1
1
1
1
-
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
7
-
1
-
3
2
1
-
-
-
1
-
-
1
-
13
1
1
1
1
-
1
1
-
1
-
-
-
-
-
-
733544
Nandanwar
Enhanced stability of newly is ...
Brevibacillus reuszeri, Brevibacillus reuszeri HSN1
Biotechnol. Appl. Biochem.
60
305-315
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-
1
-
-
1
-
-
1
-
-
-
2
-
4
-
-
1
-
-
-
-
1
4
1
1
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
1
4
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
733554
Duan
Cloning, expression, character ...
Pseudomonas sp., Pseudomonas sp. QR-101
Biotechnol. Lett.
34
1101-1106
2012
-
-
1
-
-
-
-
-
-
-
1
2
-
2
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
707251
Martínez-Rodríguez
Carbamoylases: characteristics ...
Pseudomonas sp. ON4a
Appl. Microbiol. Biotechnol.
85
441-458
2010
-
-
1
-
-
-
5
-
-
4
3
5
-
1
-
-
1
-
-
-
1
-
5
1
1
-
-
4
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
-
4
3
5
-
-
-
1
-
-
1
-
5
1
1
-
-
4
1
1
-
-
-
-
-
-
4
4
711551
Pozo-Dengra
Evaluation of substrate promis ...
Geobacillus stearothermophilus
Biotechnol. Prog.
26
954-959
2010
3
-
1
-
-
-
12
15
-
4
-
-
-
3
-
-
1
1
-
-
9
-
15
-
1
-
1
15
1
-
-
-
-
-
-
3
-
1
-
-
-
-
-
12
-
15
-
4
-
-
-
-
-
1
-
-
9
-
15
-
1
-
1
15
1
-
-
-
-
-
-
-
15
15
713443
Yen
-
Synthesis of L-homophenylalani ...
Geobacillus kaustophilus
Process Biochem.
45
667-674
2010
-
1
1
-
-
1
1
-
-
-
1
-
-
1
-
-
1
-
-
-
2
-
1
-
3
1
2
-
1
1
1
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
1
-
-
2
-
1
-
3
1
2
-
1
1
1
-
-
-
-
-
-
-
712720
Mei
New gene cluster from the ther ...
Bacillus fordii
J. Microbiol. Biotechnol.
19
1497-1505
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
687885
Kao
A novel hydantoinase process u ...
Geobacillus kaustophilus, Geobacillus kaustophilus CCRC 11223
J. Biotechnol.
134
231-239
2008
-
1
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690255
Martinez-Rodriguez
Crystallization and preliminar ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus CECT43
Acta Crystallogr. Sect. F
64
1135-1138
2008
-
-
1
1
-
-
-
-
-
-
3
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689824
Hsu
-
Stereoselective synthesis of L ...
Geobacillus kaustophilus, Geobacillus kaustophilus BCRC11223
Process Biochem.
42
856-862
2007
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672447
Martinez-Rodriguez
Thermodynamic and mutational s ...
Sinorhizobium meliloti
Biochimie
88
837-847
2006
-
-
1
-
7
-
-
5
-
3
1
-
-
3
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
5
-
3
1
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
675499
Martinez-Rodriguez
Molecular cloning and biochemi ...
Sinorhizobium meliloti
J. Mol. Microbiol. Biotechnol.
9
16-25
2005
4
1
1
-
-
-
10
10
-
-
3
-
-
3
-
-
1
-
-
-
1
-
10
1
1
1
-
-
1
1
-
-
-
-
-
4
1
1
-
-
-
-
-
10
-
10
-
-
3
-
-
-
-
1
-
-
1
-
10
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
671503
Ohmachi
A novel N-carbamoyl-L-amino ac ...
Pseudomonas sp.
Appl. Microbiol. Biotechnol.
65
686-693
2004
2
-
1
-
-
-
5
-
-
1
2
1
-
3
-
-
1
-
1
-
2
-
8
1
1
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
5
-
-
-
1
2
1
-
-
-
1
1
-
2
-
8
1
1
-
-
-
1
-
-
-
-
-
-
-
-
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288950
Hartley
Over-production of hydantoinas ...
Agrobacterium tumefaciens
Appl. Microbiol. Biotechnol.
57
43-49
2001
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288949
Pietzsch
Purification of recombinant hy ...
Paenarthrobacter aurescens
J. Chromatogr.
737
179-186
2000
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-
1
-
-
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-
-
-
-
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3
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288948
Wilms
Cloning, nucleotide sequence a ...
Paenarthrobacter aurescens
J. Biotechnol.
68
101-113
1999
-
-
1
-
-
-
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-
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-
2
-
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4
-
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1
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6
1
1
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1
5
1
1
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288947
Ishikawa
Mechanism of stereospecific co ...
Pseudomonas sp.
Biosci. Biotechnol. Biochem.
61
185-187
1997
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-
-
-
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2
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2
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-
288946
Ishikawa
N-carbamyl-L-amino acid amidoh ...
Pseudomonas sp., Pseudomonas sp. NS671
Biosci. Biotechnol. Biochem.
60
612-615
1996
-
-
1
-
-
-
1
-
-
3
2
-
-
7
-
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1
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1
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13
1
1
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1
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1
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13
1
1
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1
-
1
1
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-
-
-
-
288945
Ogawa
Purification and characterizat ...
Achromobacter xylosoxidans
Appl. Microbiol. Biotechnol.
43
1039-1043
1995
-
-
-
-
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-
18
11
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3
2
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2
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18
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19
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209293
Ishikawa
-
Microbial conversion of DL-5-s ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus NS1122A
Biosci. Biotechnol. Biochem.
58
265-270
1994
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3
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2
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