Literature summary for 3.5.1.87 extracted from

  • Nandanwar, H.S.; Vohra, R.M.; Hoondal, G.S.
    Trimeric l-N-carbamoylase from newly isolated Brevibacillus reuszeri HSN1: a potential biocatalyst for production of l-?-amino acids (2013), Biotechnol. Appl. Biochem., 60, 219-230.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme shows promise as a potential biocatalyst for L-alpha-amino acid production Brevibacillus reuszeri

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 54% inhibition at 2 mM Brevibacillus reuszeri
4-hydroxymercuribenzoate complete inhibition at 1 mM Brevibacillus reuszeri
EDTA 15% inhibition at 5 mM, 27% at 10 mM Brevibacillus reuszeri
Mercuric chloride complete inhibition at 1 mM Brevibacillus reuszeri
additional information no inhibition by PMSF and DTT Brevibacillus reuszeri
N-bromosuccinimide complete inhibition at 1 mM Brevibacillus reuszeri
Zn2+ weak inhibition at 1 mM Brevibacillus reuszeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13.5
-
N-carbamoyl-L-methionine pH 7.5, 40°C Brevibacillus reuszeri

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates at 0.08 mM Brevibacillus reuszeri
Mn2+ enzyme activity is enhanced by 73% at 0.8 mM Mn2+ Brevibacillus reuszeri
additional information no significant effect by Fe2+, Ca2+, and Ni2+ at low concentrations, overview Brevibacillus reuszeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
132000
-
gel filtration Brevibacillus reuszeri
44300
-
3 * 44300, SDS-PAGE Brevibacillus reuszeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an N-carbamoyl-L-2-amino acid + H2O Brevibacillus reuszeri
-
an L-2-amino acid + NH3 + CO2
-
?

Organism

Organism UniProt Comment Textmining
Brevibacillus reuszeri
-
-
-
Brevibacillus reuszeri HSN1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 60fold to homogeneity by heat treatment at 70°C for 2 min, anion exchange chromatography of the supernatant, hydrophobic interaction chromatography, ultrafiltration, and gel filtration Brevibacillus reuszeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
145
-
purified enzyme, pH 7.5, 40°C Brevibacillus reuszeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows stereospecificity and a broad substrate specificity. No or poor activitz with N-carbamoyl-alpha-alanine, N-carbamoyl-glycine, N-carbamoyl-beta-alanine, and N-carbamoyl-alpha-aminoisobutyrate Brevibacillus reuszeri ?
-
?
additional information the enzyme shows stereospecificity and a broad substrate specificity. No or poor activitz with N-carbamoyl-alpha-alanine, N-carbamoyl-glycine, N-carbamoyl-beta-alanine, and N-carbamoyl-alpha-aminoisobutyrate Brevibacillus reuszeri HSN1 ?
-
?
N-carbamoyl-L-methionine + H2O preferred substrate Brevibacillus reuszeri L-methionine + CO2 + NH3
-
?
N-carbamoyl-L-methionine + H2O preferred substrate Brevibacillus reuszeri HSN1 L-methionine + CO2 + NH3
-
?
N-carbamoyl-L-tryptophan + H2O
-
Brevibacillus reuszeri L-tryptophan + CO2 + NH3
-
?
N-carbamoyl-L-tryptophan + H2O
-
Brevibacillus reuszeri HSN1 L-tryptophan + CO2 + NH3
-
?
N-carbamoyl-L-phenylalanine + H2O
-
Brevibacillus reuszeri L-phenylalanine + CO2 + NH3
-
?
N-carbamoyl-L-phenylalanine + H2O
-
Brevibacillus reuszeri HSN1 L-phenylalanine + CO2 + NH3
-
?
N-carbamoyl-L-valine + H2O
-
Brevibacillus reuszeri L-valine + CO2 + NH3
-
?
N-carbamoyl-L-valine + H2O
-
Brevibacillus reuszeri HSN1 L-valine + CO2 + NH3
-
?
an N-carbamoyl-L-2-amino acid + H2O
-
Brevibacillus reuszeri an L-2-amino acid + NH3 + CO2
-
?
N-carbamoyl-D,L-serine + H2O
-
Brevibacillus reuszeri L-serine + CO2 + NH3
-
?
N-carbamoyl-L-phenylglycine + H2O
-
Brevibacillus reuszeri L-phenylglycine + CO2 + NH3
-
?

Subunits

Subunits Comment Organism
homotrimer 3 * 44300, SDS-PAGE Brevibacillus reuszeri

Synonyms

Synonyms Comment Organism
L-N-carbamoylase
-
Brevibacillus reuszeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Brevibacillus reuszeri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 75 activity range, profile overview Brevibacillus reuszeri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified enzyme, 60 min, about 86% activity remaining, half-life is 180 min Brevibacillus reuszeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Brevibacillus reuszeri

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 activity range, profile overview Brevibacillus reuszeri

pI Value

Organism Comment pI Value Maximum pI Value
Brevibacillus reuszeri isoelectric focusing
-
5.45