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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 2.7.1.146Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.146- 395267, 395269, 727486, 760433, 761560
Show all pathways known for 2.7.1.146Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.1461818fold to homogeneity by ultracentrifugation, anion exchange, ADP affinity, and cation exchange chromatography, and gel filtration 661677
Show all pathways known for 2.7.1.146Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.146by using nickel-nitrilotriacetic acid affinity chromatography and gel filtration 698924
Show all pathways known for 2.7.1.146Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.146Ni-NTA column chromatography 698924
Show all pathways known for 2.7.1.146Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.146partial 395242, 395266
Show all pathways known for 2.7.1.146Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.146recombinant enzyme 3.5fold from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by heat treatment at 80°C for 30 min, anion exchange chromatography, and dialysis, followed by hydrophobic interaction chromatography, and again dialysis 761560
Show all pathways known for 2.7.1.146Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.146recombinant enzyme from Escherichia coli strain JM109 by ammonium sulfate fractionation, adsorption and ion exchange chromatography, and gel filtration 660573
Show all pathways known for 2.7.1.146Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.146recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography 761465
Show all pathways known for 2.7.1.146Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.146recombinant N-terminally His6-tagged enzyme from Escherichia coli strains BL21(DE3) and BL21-C41(DE3) by heat treatment at 50°C for 30 min, nickel affinity chromatography, dialysis, and anion exchange chromatography 760433
Show all pathways known for 2.7.1.146Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.146recombinant N-terminally His6-tagged enzyme from Escherichia coli strains BL21(DE3) and BL21-C41(DE3) by nickel affinity chromatography, and dialysis 760433
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