BRENDA - Enzyme Database
show all sequences of 2.7.1.146

Archaeal ADP-dependent phosphofructokinase: expression, purification, crystallization and preliminary crystallographic analysis

Jeong, J.J.; Fushinobu, S.; Ito, S.; Shoun, H.; Wakagi, T.; Acta Crystallogr. Sect. D 59, 1327-1329 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109
Thermococcus litoralis
Crystallization (Commentary)
Crystallization
Organism
purified recombinant enzyme, sitting drop vapour diffusion method, 0.005 ml of 10 mg/ml protein in 150 mM NaCl, 5 mM Tris-HCl, pH 7.5, is mixed with an equal volume of reservoir solution containing 4 M NaCl, 100 mM Tris-HCl, pH 7.5, 1 week at 25░C, derivatization by soaking for 2 days in 2.85 mM K2PtCl4, 2.8 mM HAuCl4, and 2 mM 4-hydroxymercuribenzoic acid or 0.4 mM 4-hydroxymercuribenzenesulfonic acid, cryoprotection by 20% glycerol in mother liquor, X-ray diffraction structure determination and analysis at 2.6 A resolution
Thermococcus litoralis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.56
-
D-fructose 6-phosphate
-
Thermococcus litoralis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Thermococcus litoralis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
2 * 52000, recombinant enzyme, SDS-PAGE
Thermococcus litoralis
125000
-
recombinant enzyme, gel filtration
Thermococcus litoralis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP + D-fructose 6-phosphate
Thermococcus litoralis
-
AMP + D-fructose 1,6-bisphosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermococcus litoralis
Q977Q3
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli strain JM109 by ammonium sulfate fractionation, adsorption and ion exchange chromatography, and gel filtration
Thermococcus litoralis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
157
-
purified recombinant enzyme
Thermococcus litoralis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + D-fructose 6-phosphate
-
660573
Thermococcus litoralis
AMP + D-fructose 1,6-bisphosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 52000, recombinant enzyme, SDS-PAGE
Thermococcus litoralis
Cofactor
Cofactor
Commentary
Organism
Structure
ADP
-
Thermococcus litoralis
additional information
no activity with ATP
Thermococcus litoralis
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109
Thermococcus litoralis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ADP
-
Thermococcus litoralis
additional information
no activity with ATP
Thermococcus litoralis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant enzyme, sitting drop vapour diffusion method, 0.005 ml of 10 mg/ml protein in 150 mM NaCl, 5 mM Tris-HCl, pH 7.5, is mixed with an equal volume of reservoir solution containing 4 M NaCl, 100 mM Tris-HCl, pH 7.5, 1 week at 25░C, derivatization by soaking for 2 days in 2.85 mM K2PtCl4, 2.8 mM HAuCl4, and 2 mM 4-hydroxymercuribenzoic acid or 0.4 mM 4-hydroxymercuribenzenesulfonic acid, cryoprotection by 20% glycerol in mother liquor, X-ray diffraction structure determination and analysis at 2.6 A resolution
Thermococcus litoralis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.56
-
D-fructose 6-phosphate
-
Thermococcus litoralis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Thermococcus litoralis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
2 * 52000, recombinant enzyme, SDS-PAGE
Thermococcus litoralis
125000
-
recombinant enzyme, gel filtration
Thermococcus litoralis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP + D-fructose 6-phosphate
Thermococcus litoralis
-
AMP + D-fructose 1,6-bisphosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli strain JM109 by ammonium sulfate fractionation, adsorption and ion exchange chromatography, and gel filtration
Thermococcus litoralis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
157
-
purified recombinant enzyme
Thermococcus litoralis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + D-fructose 6-phosphate
-
660573
Thermococcus litoralis
AMP + D-fructose 1,6-bisphosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 52000, recombinant enzyme, SDS-PAGE
Thermococcus litoralis
Other publictions for EC 2.7.1.146
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727486
Castro-Fernandez
Bifunctional ADP-dependent pho ...
Methanococcus maripaludis
FEBS J.
281
2017-2029
2014
1
-
1
1
-
-
6
6
-
5
-
2
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
5
2
2
-
-
4
-
4
1
-
1
-
1
-
-
4
6
4
6
-
5
-
2
-
-
-
1
-
-
-
-
11
-
-
-
-
5
2
2
-
-
-
-
-
-
6
6
721764
Merino
Catalytic and regulatory roles ...
Pyrococcus horikoshii
Biochimie
94
516-524
2012
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
698924
Currie
ADP-dependent 6-phosphofructok ...
Pyrococcus horikoshii, Pyrococcus horikoshii OT-3
J. Biol. Chem.
284
22664-22671
2009
-
-
1
1
15
-
3
30
-
7
-
1
-
6
-
-
1
-
-
-
-
-
11
-
1
-
-
16
-
-
-
5
-
-
-
-
-
2
6
2
29
-
-
3
-
58
-
7
-
1
-
-
-
2
-
-
-
-
11
-
1
-
-
30
-
-
-
-
-
1
1
-
30
30
686707
Merino
Specificity evolution of the A ...
Methanocaldococcus jannaschii
FEBS J.
275
4033-4044
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661677
Hansen
ADP-dependent 6-phosphofructok ...
Archaeoglobus fulgidus
Extremophiles
8
29-35
2004
1
-
-
-
-
-
1
6
1
6
3
1
-
4
-
-
1
-
-
-
2
1
3
2
1
1
2
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
1
-
6
1
6
3
1
-
-
-
1
-
-
2
1
3
2
1
1
2
-
1
1
-
-
-
-
-
-
-
-
660573
Jeong
Archaeal ADP-dependent phospho ...
Thermococcus litoralis
Acta Crystallogr. Sect. D
59
1327-1329
2003
-
-
1
1
-
-
-
1
-
1
2
1
-
3
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
-
-
-
-
-
1
-
1
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395244
Sakuraba
ADP-dependent glucokinase/phos ...
Methanocaldococcus jannaschii
J. Biol. Chem.
277
12495-12498
2002
-
-
1
-
-
-
-
-
-
6
2
1
-
5
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
6
2
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395242
Kengen
ADP-dependent glucokinase and ...
Pyrococcus furiosus
Methods Enzymol.
331
41-53
2001
-
-
1
-
-
-
2
-
-
2
2
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
2
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395269
Verhees
ADP-dependent phosphofructokin ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
J. Bacteriol.
183
7145-7153
2001
-
-
1
-
-
-
6
3
-
4
2
4
-
13
-
-
1
-
-
-
1
-
8
1
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
6
-
3
-
6
3
4
-
-
-
2
-
-
2
-
8
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
395270
Ronimus
Sequencing, expression, charac ...
Thermococcus zilligii
Biochim. Biophys. Acta
1517
384-391
2001
-
-
1
-
-
-
-
-
-
-
3
1
-
7
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721449
Labes
Sugar utilization in the hyper ...
Archaeoglobus fulgidus, Archaeoglobus fulgidus 7324
Arch. Microbiol.
176
329-338
2001
-
-
-
-
-
-
-
2
-
-
-
1
-
9
-
-
-
-
-
2
2
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
2
2
-
2
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
395266
Tuininga
Molecular and biochemical char ...
Pyrococcus furiosus
J. Biol. Chem.
274
21023-21028
1999
-
-
1
-
-
-
2
-
-
2
2
1
-
4
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
2
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395267
Ronimus
Purification and characterizat ...
Thermococcus zilligii
Extremophiles
3
121-129
1999
-
-
-
-
-
-
-
-
-
3
2
1
-
2
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722596
Kengen
Evidence for the operation of ...
Pyrococcus furiosus
J. Biol. Chem.
269
17537-17541
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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