EC Number   |
|---|
    2.7.7.13 | HisTrap Ni affinity column chromatography and Superdex 200 gel filtration |
| 2.7.7.13 | Ni-affinity column |
| 2.7.7.13 | Ni-NTA column chromatography, and Superdex 200 gel filtration |
| 2.7.7.13 | protamine sulfate, Sephadex G-25, DEAE-cellulose |
| 2.7.7.13 | recombinant enzyme from Escherichia coli strain BL21(DE3) |
| 2.7.7.13 | recombinant enzyme from Escherichia coli to homogeneity by glutathione sepharose affinity chromatography |
| 2.7.7.13 | recombinant enzyme, Ni-nitrilotriacetic acid column, Superdex 200 |
| 2.7.7.13 | recombinant enzyme, Q-Sepharose FF, Phenyl-Sepharose, diafiltration, Sephadex G 75, Sephadex G 200, ammonium sulfate |
| 2.7.7.13 | recombinant enzyme, ultrafiltration, Q-Sepharose |
| 2.7.7.13 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
