2.7.7.13 - 2.7.7.13 acid treatment, protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-200, hydroxylapatite, Blue Sepharose 2.7.7.13 affinity chromatography using Talon-Co2+ resin, gel filtration 2.7.7.13 batch absorption to nickel-agarose 2.7.7.13 Biogel, Q-Sepharose, Sephacryl-200 2.7.7.13 blue-Sepharose, DEAE-Sepharose, phenyl-Sepharose, agarose-GTP affinity chromatography 2.7.7.13 DEAE-52, ammonium sulfate, propyl-agarose, methyl-agrose, Sephadex G-200, hydroxyapatite 2.7.7.13 GST-fusion protein, glutathione-Sepharose 4B beads 2.7.7.13 Hi-Trap column chromatography 2.7.7.13 HIS-Select nickel affinity gel chromatography 2.7.7.13 HisTrap Ni affinity column chromatography and Superdex 200 gel filtration 2.7.7.13 Ni-affinity column 2.7.7.13 Ni-NTA column chromatography, and Superdex 200 gel filtration 2.7.7.13 protamine sulfate, Sephadex G-25, DEAE-cellulose 2.7.7.13 recombinant enzyme from Escherichia coli strain BL21(DE3) 2.7.7.13 recombinant enzyme from Escherichia coli to homogeneity by glutathione sepharose affinity chromatography 2.7.7.13 recombinant enzyme, Ni-nitrilotriacetic acid column, Superdex 200 2.7.7.13 recombinant enzyme, Q-Sepharose FF, Phenyl-Sepharose, diafiltration, Sephadex G 75, Sephadex G 200, ammonium sulfate 2.7.7.13 recombinant enzyme, ultrafiltration, Q-Sepharose 2.7.7.13 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 2.7.7.13 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity 2.7.7.13 recombinant His-tagged enzyme from Escherichia coli strain Origami (DE3) pLysS by nickel affinity chromatography and gel filtration 2.7.7.13 recombinant His-tagged PH0925 protein from Escherichia coli strain BL21-Codon Plus(DE3)-RIL by nickel affinity chromatography 2.7.7.13 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by metal affinity chromatograhy