EC Number |
Reference |
---|
2.7.11.24 | recombinant His-tagged enzyme from Escherichia coli by affinity chromatography |
690853 |
2.7.11.24 | recombinant His-tagged isozyme p38alpha MAPK from Escherichia coli by nickel affinity chromatography |
691290 |
2.7.11.24 | recombinant His-tagged MPK6 by metal affinity chromatography, the His-tag is cleaved off by TEV protease |
741070 |
2.7.11.24 | recombinant His-tagged p38 isozyme alpha from Escherichia coli strain BL21(DE3) by nickel affinity and ion exchange chromatography to over 98% purity, removal of the His-tag by thrombin is required for propper enzyme purification and activation |
663620 |
2.7.11.24 | recombinant His-tagged p38alpha from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography to homogeneity |
693135 |
2.7.11.24 | recombinant His-tagged p38alpha MAP kinase from Escherichia coli strain BL21(DE3) by nickel chelate affinity chromatohgraphy, dialysis, and ion exchange chromatography to homogeneity, the His-tag is cleaved off by thrombin |
666815 |
2.7.11.24 | recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange chromatography and gel filtration for non-tagged proteins, His6-tagged proteins are purified by nickel affinity chromatography |
693668 |