2.7.11.24	-	490760, 491732, 678159, 680249	
2.7.11.24	a SP Fastflow column, a Mono S column, and a Sephacryl S200 column are used	704471	
2.7.11.24	ammonium sulfate precipitation, Q Sepharose column chromatography, phenyl Sepharose column chromatography, Mono QTM column chromatography, poly-L-Lys-agarose column chromatography, and Sephadex G25 gel filtration	762159	
2.7.11.24	elicitor-induced MAPK is purified from the protein extract of cells by anion-exchange chromatography	702773	
2.7.11.24	ERK1 and p38alpha kinase purified by Ni–NTA column	677283	
2.7.11.24	gel filtration	680896	
2.7.11.24	glutathione-Sepharose column chromatography	761895	
2.7.11.24	GST- and His-tags are used in the purification experiments	701666	
2.7.11.24	His-tagged p38 MAP kinase	690672	
2.7.11.24	Ni-NTA agarose column chromatography	761905	
2.7.11.24	nickel column chromatography	762023	
2.7.11.24	p38alpha active mutants, by gel filtration, on Ni2+-column	677321	
2.7.11.24	partially purified from maize by Q-Sepharose FF, Phenyl-Sepharose FF, Resource Q, Mono QTM 5/50 GL, poly-L-lysine agarose, and Superdex 75 prep-grade columns	706382	
2.7.11.24	purification of synaptosomes	666131	
2.7.11.24	recombinant 0P-ERK2 wild-type and mutants from Escherichia coli strain BL21(DE3)	740069	
2.7.11.24	recombinant C-terminally His6-tagged p38alpha from Escherichia coli strain BL21 (DE3) RIL by nickel affinity chromatography and gel filtration	740712	
2.7.11.24	recombinant GST-tagged MPK3 by affinity chromatography from Escherichia coli	740240	
2.7.11.24	recombinant GST-tagged MPK4 by affinity chromatography from Escherichia coli	740240	
2.7.11.24	recombinant GST-tagged MPK6 by affinity chromatography from Escherichia coli	740240	
2.7.11.24	recombinant GST-tagged p38 isozyme alpha from Escherichia coli strain BL21(DE3) by two steps of ion exchange chromatography to homogeneity, the recombinant enzyme is detagged	664894	
2.7.11.24	recombinant His-tagged enzyme from Escherichia coli by affinity chromatography	690853	
2.7.11.24	recombinant His-tagged isozyme p38alpha MAPK from Escherichia coli by nickel affinity chromatography	691290	
2.7.11.24	recombinant His-tagged MPK6 by metal affinity chromatography, the His-tag is cleaved off by TEV protease	741070	
2.7.11.24	recombinant His-tagged p38 isozyme alpha from Escherichia coli strain BL21(DE3) by nickel affinity and ion exchange chromatography to over 98% purity, removal of the His-tag by thrombin is required for propper enzyme purification and activation	663620	
2.7.11.24	recombinant His-tagged p38alpha from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography to homogeneity	693135	
2.7.11.24	recombinant His-tagged p38alpha MAP kinase from Escherichia coli strain BL21(DE3) by nickel chelate affinity chromatohgraphy, dialysis, and ion exchange chromatography to homogeneity, the His-tag is cleaved off by thrombin	666815	
2.7.11.24	recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange chromatography and gel filtration for non-tagged proteins, His6-tagged proteins are purified by nickel affinity chromatography	693668