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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 2.7.1.48Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.48recombinant enzyme using glutathione-S-transferase tag 660609
Show all pathways known for 2.7.1.48Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.48recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography 758717
Show all pathways known for 2.7.1.48Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.48recombinant His-tagged uridine kinase, Ni-nitrilotriacetic acid-Sepharose 641654
Show all pathways known for 2.7.1.48Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.48recombinant N-terminally His-tagged isozyme UCK1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 759866
Show all pathways known for 2.7.1.48Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.48recombinant N-terminally His-tagged isozyme UCK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 759866
Show all pathways known for 2.7.1.48Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.48recombinant protein using His-tag 661576
Show all pathways known for 2.7.1.48Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.48recombinant ttCK from Escherichia coli strain Rosetta(DE3) by heat treatment at 60°C for 20 min, hydrophobic interaction chromatography, and gel filtration 721643
Show all pathways known for 2.7.1.48Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.48recombinant wild-type and mutant enzymes from Escherichia coli strain Rosetta(DE3) by heat treatment at 70°C for 20 min, followed by hydrophobic interaction chromatography, and two different steps of gel filtration 758717
Show all pathways known for 2.7.1.48Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.48streptomycin, ammonium sulfate, calcium phosphate, DEAE-cellulose 641639
Show all pathways known for 2.7.1.48Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.48streptomycin, ammonium sulfate, calcium phosphate, Sephadex G-200 641643, 641646
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