2.7.1.48 - 641640, 641643 2.7.1.48 ammonium sulfate precipitation, Toyopearl Phenyl-650 M column chromatography, Toyopearl SP-650 column chromatography and Superdex 200 gel filtration 739594 2.7.1.48 ammonium sulfate, Pb2+-precipitation 641651 2.7.1.48 DE-52, ammonium sulfate, ATP-agarose column 641647 2.7.1.48 DEAE-cellulose, ammonium sulfate, hydroxyapatite 641645, 641649 2.7.1.48 immobilized metal ion adsorption chromatography (Ni2+) 706070 2.7.1.48 Ni-NTA agarose column chromatography and Sephadex G25 gel filtration 737778 2.7.1.48 partial purification 641642 2.7.1.48 protamine sulfate, CM-cellulose, ammonium sulfate, DEAE-cellulose, zone electrophoresis 641638 2.7.1.48 recombinant C-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography followed by ion-exchange chromatography 758919 2.7.1.48 recombinant enzyme using glutathione-S-transferase tag 660609 2.7.1.48 recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography 758717 2.7.1.48 recombinant His-tagged uridine kinase, Ni-nitrilotriacetic acid-Sepharose 641654 2.7.1.48 recombinant N-terminally His-tagged isozyme UCK1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 759866 2.7.1.48 recombinant N-terminally His-tagged isozyme UCK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 759866 2.7.1.48 recombinant protein using His-tag 661576 2.7.1.48 recombinant ttCK from Escherichia coli strain Rosetta(DE3) by heat treatment at 60°C for 20 min, hydrophobic interaction chromatography, and gel filtration 721643 2.7.1.48 recombinant wild-type and mutant enzymes from Escherichia coli strain Rosetta(DE3) by heat treatment at 70°C for 20 min, followed by hydrophobic interaction chromatography, and two different steps of gel filtration 758717 2.7.1.48 streptomycin, ammonium sulfate, calcium phosphate, DEAE-cellulose 641639 2.7.1.48 streptomycin, ammonium sulfate, calcium phosphate, Sephadex G-200 641643, 641646 2.7.1.48 streptomycin, ammonium sulfate, DEAE-cellulose 641648 2.7.1.48 ultracentrifugation, DEAE-cellulose, affinity chromatography on 5'amino-5'-deoxyuridine 641644