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Literature summary for 2.7.1.48 extracted from
- Uridine-cytidine kinase from Novikoff ascites rat tumor and Bacillus stearothermophilus (1978), Methods Enzymol., 51, 299-307.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CTP | enzyme shows a temperature dependence of CTP inhibition | Geobacillus stearothermophilus |  |
| CTP | 0.56 mM, 50% inhibition | Rattus norvegicus | |
| UTP | - |
Geobacillus stearothermophilus | |
| UTP | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
uridine | pH 7.5, 10°C | Geobacillus stearothermophilus | |
| 0.64 | - |
uridine | pH 7.5, 37°C | Geobacillus stearothermophilus | |
| 1.6 | - |
uridine | pH 7.5, 60°C | Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for full activity | Rattus norvegicus | |
| Mg2+ | required for full activity | Geobacillus stearothermophilus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + uridine | Rattus norvegicus | first enzyme of pyrimidine salvage pathway | ADP + UMP | - |
? | |
| ATP + uridine | Geobacillus stearothermophilus | first enzyme of pyrimidine salvage pathway | ADP + UMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
| streptomycin, ammonium sulfate, calcium phosphate, Sephadex G-200 | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + uridine = ADP + UMP | ping-pong mechanism | Rattus norvegicus | |
| ATP + uridine = ADP + UMP | ping-pong mechanism | Geobacillus stearothermophilus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Novikoff ascites tumor cell | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.88 | - |
- |
Rattus norvegicus |
| 4.33 | - |
- |
Geobacillus stearothermophilus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, at least 6 months, no loss of activity | Rattus norvegicus |
| 4°C, at least 1 month, no loss of activity | Rattus norvegicus |
| 4°C, sterile conditions, at least 2 years, no loss of activity enzyme | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + uridine | very low activity with CTP and UTP | Rattus norvegicus | ADP + UMP | - |
? | |
| ATP + uridine | very low activity with CTP and UTP | Geobacillus stearothermophilus | ADP + UMP | - |
? | |
| ATP + uridine | first enzyme of pyrimidine salvage pathway | Rattus norvegicus | ADP + UMP | - |
? | |
| ATP + uridine | first enzyme of pyrimidine salvage pathway | Geobacillus stearothermophilus | ADP + UMP | - |
? | |
| dATP + uridine | - |
Rattus norvegicus | dADP + UMP | - |
? | |
| dATP + uridine | - |
Geobacillus stearothermophilus | dADP + UMP | - |
? | |
| dCTP + uridine | weak activity | Rattus norvegicus | dCDP + UMP | - |
? | |
| dCTP + uridine | weak activity | Geobacillus stearothermophilus | dCDP + UMP | - |
? | |
| dGTP + uridine | - |
Rattus norvegicus | dGDP + UMP | - |
? | |
| dGTP + uridine | - |
Geobacillus stearothermophilus | dGDP + UMP | - |
? | |
| dTTP + uridine | weak activity | Rattus norvegicus | dTDP + UMP | - |
? | |
| dTTP + uridine | weak activity | Geobacillus stearothermophilus | dTDP + UMP | - |
? | |
| dUTP + uridine | - |
Rattus norvegicus | dUDP + UMP | - |
? | |
| dUTP + uridine | - |
Geobacillus stearothermophilus | dUDP + UMP | - |
? | |
| GTP + uridine | weak activity | Rattus norvegicus | UMP + GDP | - |
? | |
| GTP + uridine | weak activity | Geobacillus stearothermophilus | UMP + GDP | - |
? | |
| ITP + uridine | weak activity | Rattus norvegicus | IDP + UMP | - |
? | |
| ITP + uridine | weak activity | Geobacillus stearothermophilus | IDP + UMP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Geobacillus stearothermophilus |
| 60 | - |
higher activity than at 10°C and 37°C | Geobacillus stearothermophilus |
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