Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CTP | enzyme shows a temperature dependence of CTP inhibition | Geobacillus stearothermophilus | |
CTP | 0.56 mM, 50% inhibition | Rattus norvegicus | |
UTP | - |
Geobacillus stearothermophilus | |
UTP | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
uridine | pH 7.5, 10°C | Geobacillus stearothermophilus | |
0.64 | - |
uridine | pH 7.5, 37°C | Geobacillus stearothermophilus | |
1.6 | - |
uridine | pH 7.5, 60°C | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for full activity | Rattus norvegicus | |
Mg2+ | required for full activity | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + uridine | Rattus norvegicus | first enzyme of pyrimidine salvage pathway | ADP + UMP | - |
? | |
ATP + uridine | Geobacillus stearothermophilus | first enzyme of pyrimidine salvage pathway | ADP + UMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
streptomycin, ammonium sulfate, calcium phosphate, Sephadex G-200 | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + uridine = ADP + UMP | ping-pong mechanism | Rattus norvegicus | |
ATP + uridine = ADP + UMP | ping-pong mechanism | Geobacillus stearothermophilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Novikoff ascites tumor cell | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.88 | - |
- |
Rattus norvegicus |
4.33 | - |
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, at least 6 months, no loss of activity | Rattus norvegicus |
4°C, at least 1 month, no loss of activity | Rattus norvegicus |
4°C, sterile conditions, at least 2 years, no loss of activity enzyme | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + uridine | very low activity with CTP and UTP | Rattus norvegicus | ADP + UMP | - |
? | |
ATP + uridine | very low activity with CTP and UTP | Geobacillus stearothermophilus | ADP + UMP | - |
? | |
ATP + uridine | first enzyme of pyrimidine salvage pathway | Rattus norvegicus | ADP + UMP | - |
? | |
ATP + uridine | first enzyme of pyrimidine salvage pathway | Geobacillus stearothermophilus | ADP + UMP | - |
? | |
dATP + uridine | - |
Rattus norvegicus | dADP + UMP | - |
? | |
dATP + uridine | - |
Geobacillus stearothermophilus | dADP + UMP | - |
? | |
dCTP + uridine | weak activity | Rattus norvegicus | dCDP + UMP | - |
? | |
dCTP + uridine | weak activity | Geobacillus stearothermophilus | dCDP + UMP | - |
? | |
dGTP + uridine | - |
Rattus norvegicus | dGDP + UMP | - |
? | |
dGTP + uridine | - |
Geobacillus stearothermophilus | dGDP + UMP | - |
? | |
dTTP + uridine | weak activity | Rattus norvegicus | dTDP + UMP | - |
? | |
dTTP + uridine | weak activity | Geobacillus stearothermophilus | dTDP + UMP | - |
? | |
dUTP + uridine | - |
Rattus norvegicus | dUDP + UMP | - |
? | |
dUTP + uridine | - |
Geobacillus stearothermophilus | dUDP + UMP | - |
? | |
GTP + uridine | weak activity | Rattus norvegicus | UMP + GDP | - |
? | |
GTP + uridine | weak activity | Geobacillus stearothermophilus | UMP + GDP | - |
? | |
ITP + uridine | weak activity | Rattus norvegicus | IDP + UMP | - |
? | |
ITP + uridine | weak activity | Geobacillus stearothermophilus | IDP + UMP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Geobacillus stearothermophilus |
60 | - |
higher activity than at 10°C and 37°C | Geobacillus stearothermophilus |