EC Number   |
Posttranslational Modification |
Reference |
|---|
   2.3.2.5 | glycoprotein |
- |
487989, 488000, 660424, 718904 |
| 2.3.2.5 | glycoprotein |
deglycosylation has no effect on enzymatic activity |
487998 |
| 2.3.2.5 | glycoprotein |
glycosylated structures of sQC are reported in PDB which exhibit divergence in the structures especially at the loop regions constituted by F146-V153 and Q295-I303. A disulfide bridge between C139 and C164 has also been observed in the glycosylated sQC. In the glycosylated structures, the position of W207 resembles the enzyme structure Conf-B |
759909 |
| 2.3.2.5 | glycoprotein |
N-glycan sugar moiety is bound to Asn49 in the recombinant enzyme. Presence or absence of glycan has no apparent effect on structure, stability, or enzymatic activity of the enzyme |
660424 |
| 2.3.2.5 | glycoprotein |
presence of terminal mannose residues, mannose and N-acetylglucosamine in a 3:1 molar ratio |
487993 |
| 2.3.2.5 | glycoprotein |
sequence contain potential N-glycosylation sites at Asn53, Asn292, and Asn352 |
677220 |
| 2.3.2.5 | glycoprotein |
sequence contains a potential glycosylation site at Asp50 |
671982 |
| 2.3.2.5 | glycoprotein |
treatment of recombinant enzyme with endoglycosidase H results in decrease of mass by 2 kDa. Deglycosylated enzyme has similar kinetic parameters for conversion of Gln-Gln and Gln-7-amido-4-methylcoumarin as native enzyme |
672479 |
| 2.3.2.5 | proteolytic modification |
sequence contains an ambiguous signal peptide cleavage site leading to a mature enzyme of 345, 340 or 335 amino acids |
677220 |
