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Literature summary for 2.3.2.5 extracted from

  • Schilling, S.; Cynis, H.; von Bohlen, A.; Hoffmann, T.; Wermann, M.; Heiser, U.; Buchholz, M.; Zunkel, K.; Demuth, H.U.
    Isolation, catalytic properties, and competitive inhibitors of the zinc-dependent murine glutaminyl cyclase (2005), Biochemistry, 44, 13415-13424.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline time-dependent inactivation Mus musculus
2,6-dipicolinic acid time-dependent inactivation Mus musculus
2-mercaptoethanol
-
Mus musculus
8-hydroxyquinoline time-dependent inactivation Mus musculus
benzimidazole
-
Mus musculus
benzylimidazole
-
Mus musculus
Citric acid inactivation at pH 5.5, Zn2+ protects Mus musculus
cysteamine competitive Mus musculus
ethanolamine
-
Mus musculus
ethylamine
-
Mus musculus
ethylenediamine
-
Mus musculus
ethylmercaptane
-
Mus musculus
imidazole
-
Mus musculus
methylimidazole
-
Mus musculus
N-diethylcysteamine
-
Mus musculus
N-dimethylcysteamine
-
Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
L-Gln-2-naphthylamide pH 8.0, 30°C Mus musculus
0.048
-
L-Gln-7-amido-4-methylcoumarin pH 8.0, 30°C Mus musculus
0.072
-
L-Gln-L-Trp-L-Ala-NH2 pH 8.0, 30°C Mus musculus
0.15
-
L-Gln-L-Gln pH 8.0, 30°C Mus musculus
0.16
-
L-Gln-L-Thr-Gly-L-Ile-NH2 pH 8.0, 30°C Mus musculus
0.17
-
L-Gln-Gly-L-Pro pH 8.0, 30°C Mus musculus
0.32
-
L-Gln-L-Arg-Gly-L-Ile-NH2 pH 8.0, 30°C Mus musculus
0.36
-
L-Gln-L-Asn-Gly-L-Ile-NH2 pH 8.0, 30°C Mus musculus
0.4
-
L-Gln-L-Ala pH 8.0, 30°C Mus musculus
0.41
-
L-Gln-Gly pH 8.0, 30°C Mus musculus
0.8
-
L-Gln-L-Glu pH 8.0, 30°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc stoichiometric amounts of zinc bound to protein. Depletion of zinc has no significant effect on protein structure, metal has a catalytic role Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q9CYK2 expressed in Pichia pastoris
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sequence contains a potential glycosylation site at Asp50 Mus musculus

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.2
-
pH 8.0, 30°C, recombinant enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Gln-2-naphthylamide
-
Mus musculus L-pyroglutamyl-2-naphthylamide + NH3
-
?
L-Gln-7-amido-4-methylcoumarin
-
Mus musculus L-pyroglutamyl-4-methylcoumarin-7-amide + NH3
-
?
L-Gln-Gly
-
Mus musculus L-pyroglutamyl-Gly + NH3
-
?
L-Gln-Gly-L-Pro
-
Mus musculus pyroglutamyl-Gly-L-Pro + NH3
-
?
L-Gln-L-Ala
-
Mus musculus L-pyroglutamyl-L-Ala + NH3
-
?
L-Gln-L-Arg-Gly-L-Ile-NH2
-
Mus musculus pyroglutamyl-L-Arg-Gly-L-Ile-NH2 + NH3
-
?
L-Gln-L-Asn-Gly-L-Ile-NH2
-
Mus musculus L-pyroglutamyl-L-Asn-Gly-L-Ile-NH2 + NH3
-
?
L-Gln-L-Gln
-
Mus musculus L-pyroglutamyl-L-Gln + NH3
-
?
L-Gln-L-Glu
-
Mus musculus L-pyroglutamyl-L-Glu + NH3
-
?
L-Gln-L-Thr-Gly-L-Ile-NH2
-
Mus musculus L-pyroglutamyl-L-Thr-Gly-L-Ile-NH2 + NH3
-
?
L-Gln-L-Trp-L-Ala-NH2
-
Mus musculus L-pyroglutamyl-L-Trp-L-Ala-NH2 + NH3
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
L-Gln-7-amido-4-methylcoumarin pH 8.0, 30°C Mus musculus
12.4
-
L-Gln-Gly pH 8.0, 30°C Mus musculus
21.6
-
L-Gln-Gly-L-Pro pH 8.0, 30°C Mus musculus
22
-
L-Gln-2-naphthylamide pH 8.0, 30°C Mus musculus
23
-
L-Gln-L-Thr-Gly-L-Ile-NH2 pH 8.0, 30°C Mus musculus
29
-
L-Gln-L-Glu pH 8.0, 30°C Mus musculus
32
-
L-Gln-L-Gln pH 8.0, 30°C Mus musculus
34
-
L-Gln-L-Arg-Gly-L-Ile-NH2 pH 8.0, 30°C Mus musculus
41
-
L-Gln-L-Trp-L-Ala-NH2 pH 8.0, 30°C Mus musculus
42
-
L-Gln-L-Ala pH 8.0, 30°C Mus musculus
68
-
L-Gln-L-Asn-Gly-L-Ile-NH2 pH 8.0, 30°C Mus musculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 stable for at least 30 min at 30°C, both native and recombinant enzyme. At pH values below or above, rapid inactivation. 0.02 mM ZnSO4 partially prevents denaturation in the acidic range Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0064
-
benzylimidazole pH 8.0, 30°C Mus musculus
0.0109
-
N-diethylcysteamine
-
Mus musculus
0.023
-
methylimidazole pH 8.0, 30°C Mus musculus
0.029
-
N-dimethylcysteamine
-
Mus musculus
0.042
-
cysteamine pH 8.0, 30°C Mus musculus
0.16
-
imidazole pH 8.0, 30°C Mus musculus
0.192
-
benzimidazole pH 8.0, 30°C Mus musculus
2.58
-
2-mercaptoethanol
-
Mus musculus
19.3
-
ethanolamine
-
Mus musculus
22
-
ethylenediamine
-
Mus musculus