Cloned (Comment) | Organism |
---|---|
- |
Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | time-dependent inactivation | Mus musculus | |
2,6-dipicolinic acid | time-dependent inactivation | Mus musculus | |
2-mercaptoethanol | - |
Mus musculus | |
8-hydroxyquinoline | time-dependent inactivation | Mus musculus | |
benzimidazole | - |
Mus musculus | |
benzylimidazole | - |
Mus musculus | |
Citric acid | inactivation at pH 5.5, Zn2+ protects | Mus musculus | |
cysteamine | competitive | Mus musculus | |
ethanolamine | - |
Mus musculus | |
ethylamine | - |
Mus musculus | |
ethylenediamine | - |
Mus musculus | |
ethylmercaptane | - |
Mus musculus | |
imidazole | - |
Mus musculus | |
methylimidazole | - |
Mus musculus | |
N-diethylcysteamine | - |
Mus musculus | |
N-dimethylcysteamine | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
L-Gln-2-naphthylamide | pH 8.0, 30°C | Mus musculus | |
0.048 | - |
L-Gln-7-amido-4-methylcoumarin | pH 8.0, 30°C | Mus musculus | |
0.072 | - |
L-Gln-L-Trp-L-Ala-NH2 | pH 8.0, 30°C | Mus musculus | |
0.15 | - |
L-Gln-L-Gln | pH 8.0, 30°C | Mus musculus | |
0.16 | - |
L-Gln-L-Thr-Gly-L-Ile-NH2 | pH 8.0, 30°C | Mus musculus | |
0.17 | - |
L-Gln-Gly-L-Pro | pH 8.0, 30°C | Mus musculus | |
0.32 | - |
L-Gln-L-Arg-Gly-L-Ile-NH2 | pH 8.0, 30°C | Mus musculus | |
0.36 | - |
L-Gln-L-Asn-Gly-L-Ile-NH2 | pH 8.0, 30°C | Mus musculus | |
0.4 | - |
L-Gln-L-Ala | pH 8.0, 30°C | Mus musculus | |
0.41 | - |
L-Gln-Gly | pH 8.0, 30°C | Mus musculus | |
0.8 | - |
L-Gln-L-Glu | pH 8.0, 30°C | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | stoichiometric amounts of zinc bound to protein. Depletion of zinc has no significant effect on protein structure, metal has a catalytic role | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9CYK2 | expressed in Pichia pastoris | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | sequence contains a potential glycosylation site at Asp50 | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.2 | - |
pH 8.0, 30°C, recombinant enzyme | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Gln-2-naphthylamide | - |
Mus musculus | L-pyroglutamyl-2-naphthylamide + NH3 | - |
? | |
L-Gln-7-amido-4-methylcoumarin | - |
Mus musculus | L-pyroglutamyl-4-methylcoumarin-7-amide + NH3 | - |
? | |
L-Gln-Gly | - |
Mus musculus | L-pyroglutamyl-Gly + NH3 | - |
? | |
L-Gln-Gly-L-Pro | - |
Mus musculus | pyroglutamyl-Gly-L-Pro + NH3 | - |
? | |
L-Gln-L-Ala | - |
Mus musculus | L-pyroglutamyl-L-Ala + NH3 | - |
? | |
L-Gln-L-Arg-Gly-L-Ile-NH2 | - |
Mus musculus | pyroglutamyl-L-Arg-Gly-L-Ile-NH2 + NH3 | - |
? | |
L-Gln-L-Asn-Gly-L-Ile-NH2 | - |
Mus musculus | L-pyroglutamyl-L-Asn-Gly-L-Ile-NH2 + NH3 | - |
? | |
L-Gln-L-Gln | - |
Mus musculus | L-pyroglutamyl-L-Gln + NH3 | - |
? | |
L-Gln-L-Glu | - |
Mus musculus | L-pyroglutamyl-L-Glu + NH3 | - |
? | |
L-Gln-L-Thr-Gly-L-Ile-NH2 | - |
Mus musculus | L-pyroglutamyl-L-Thr-Gly-L-Ile-NH2 + NH3 | - |
? | |
L-Gln-L-Trp-L-Ala-NH2 | - |
Mus musculus | L-pyroglutamyl-L-Trp-L-Ala-NH2 + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
L-Gln-7-amido-4-methylcoumarin | pH 8.0, 30°C | Mus musculus | |
12.4 | - |
L-Gln-Gly | pH 8.0, 30°C | Mus musculus | |
21.6 | - |
L-Gln-Gly-L-Pro | pH 8.0, 30°C | Mus musculus | |
22 | - |
L-Gln-2-naphthylamide | pH 8.0, 30°C | Mus musculus | |
23 | - |
L-Gln-L-Thr-Gly-L-Ile-NH2 | pH 8.0, 30°C | Mus musculus | |
29 | - |
L-Gln-L-Glu | pH 8.0, 30°C | Mus musculus | |
32 | - |
L-Gln-L-Gln | pH 8.0, 30°C | Mus musculus | |
34 | - |
L-Gln-L-Arg-Gly-L-Ile-NH2 | pH 8.0, 30°C | Mus musculus | |
41 | - |
L-Gln-L-Trp-L-Ala-NH2 | pH 8.0, 30°C | Mus musculus | |
42 | - |
L-Gln-L-Ala | pH 8.0, 30°C | Mus musculus | |
68 | - |
L-Gln-L-Asn-Gly-L-Ile-NH2 | pH 8.0, 30°C | Mus musculus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | stable for at least 30 min at 30°C, both native and recombinant enzyme. At pH values below or above, rapid inactivation. 0.02 mM ZnSO4 partially prevents denaturation in the acidic range | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
benzylimidazole | pH 8.0, 30°C | Mus musculus | |
0.0109 | - |
N-diethylcysteamine | - |
Mus musculus | |
0.023 | - |
methylimidazole | pH 8.0, 30°C | Mus musculus | |
0.029 | - |
N-dimethylcysteamine | - |
Mus musculus | |
0.042 | - |
cysteamine | pH 8.0, 30°C | Mus musculus | |
0.16 | - |
imidazole | pH 8.0, 30°C | Mus musculus | |
0.192 | - |
benzimidazole | pH 8.0, 30°C | Mus musculus | |
2.58 | - |
2-mercaptoethanol | - |
Mus musculus | |
19.3 | - |
ethanolamine | - |
Mus musculus | |
22 | - |
ethylenediamine | - |
Mus musculus |