EC Number   |
Application |
Reference |
|---|
    4.2.1.3 | biotechnology |
molecular chaperones GroEL/GroES are co-expressed with soluble, biologically active recombinant aconitase in Escherichia coli by cultivation in a bioreactor at different temperatures under optimized conditions. The yield of functional aconitase is enhanced, either in presence of co-expressed GroEL/ES or at low temperature cultivation. The outcome from the chaperone assisted folding of aconitase is more pronounced at lower temperature |
704735 |
| 4.2.1.3 | environmental protection |
use of enzyme as biomarker of oxidative damage. Exposure of oysters to Cd2+ results in elevated production of reactive oxygen species and enzyme inhibition, which is particualrly pronounced at elevated temperature |
681116 |
| 4.2.1.3 | medicine |
a high fat diet (60% kcal from fat) shows significantly increased mitochondrial aconitase activity without changes in protein level and produces increased aconitase acetylation at multiple sites |
747087 |
| 4.2.1.3 | medicine |
enzyme inhibition, e.g. by deferiprone, is helpful in Friedreich's ataxia treatment |
696992 |
| 4.2.1.3 | medicine |
it is shown that the cytosolic aconitase defect and consequent IRP1 activation occurring in Friedreich's Ataxia (FRDA) cells are reversed by the action of extramitochondrial frataxin |
703963 |
| 4.2.1.3 | medicine |
mitochondrial enzyme is present in the glandular epithelium of normal glands, hyperplastic glands, adenocarcinomatous glands, and prostatic intraepithelial neoplastic fold. There is no difference in enzyme levels between malignant and non-malignant tissues |
681864 |
| 4.2.1.3 | synthesis |
overexpression of enzyme in Escherichia coli. Presence of co-expressed GroEL reduces the aconitase over-expression drastically, however, exogenous GroEL and GroES together compensate this reduction. For over-expressing cells, growth-rate decreases by 30% at 25°C, however, in presence of co-expressed GroEL and GroES the growth rate of aconitase producing cells is enhanced by 30% at 37°C |
680182 |
