EC Number   |
Application |
Reference |
|---|
    3.2.1.4 | agriculture |
in the treatment of agricultural waste at high temperature and low pH. Utilization in the biofuel industry after thermal pre-treatment in an acidic environment (e.g., steam explosion) of corncob, sugarcane bagasse and several types of agricultural waste to hydrolyze them down to fermentable sugars |
-, 692258 |
| 3.2.1.4 | analysis |
construction of a pipeline based on Leishmania tarentolae cell-free system to characterize 30 putative thermostable endo-1,4-beta-glucanases and xylanases identified in public genomic databases. The system uses high-throughput assays for glucanase and xylanase activities that rely on solubilisation of labelled particulate substrates performed in multiwell plates |
745427 |
| 3.2.1.4 | analysis |
endo-beta-1,4-D-glucanase can be used as a marker to study root development in Arabidopsis |
666605 |
| 3.2.1.4 | analysis |
labeled Trichoderma reesei cellulase is useful as a marker for Acanthamoeba cyst wall cellulose in infected tissues |
707226 |
| 3.2.1.4 | analysis |
method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation. Cellulases are printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms are detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose, while exocellulase isoforms are detected in electrophoresis gel with 4-methylumbelliferyl-beta-D-cellobioside |
736017 |
| 3.2.1.4 | analysis |
polymerization-based assay for determining the potency of cellulolytic enzyme formulations on pretreated biomass substrates by monitoring the autofluorescence of cellulose. The one-pot method is label-free, rapid, highly sensitive, and requires only a single pipetting step. Using model enzyme formulations derived from Trichoderma reesei, Trichoderma longibrachiatum, Talaromyces emersonii and recombinant bacterial minicellulosomes from Clostridium thermocellum, enzyme performance based on differences in thermostability, cellulose-binding domain targeting, and endo/exoglucanase synergy can be differentiated |
735430 |
| 3.2.1.4 | analysis |
rapid, selective, quantitative assay based on substrate 44-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside and reaction of product 4,6-O-(3-oxobutylidene)-beta-D-cellotriose with beta-glucosidase |
744006 |
| 3.2.1.4 | analysis |
screening for thermostable cellulase using 2% carboxymethyl cellulose and congo red as an indicator at temperatures 0°C, 37°C, 45°C and 50°C,respectively. Eight isolates were selected for further screening and show the abilities to secrete cellulases by forming distinct halo zones on selective agar plate. The maximum halo zones ranging from 32 mm to 35 mm are obtained after 72 hours incubation at 50°C |
736723 |
| 3.2.1.4 | analysis |
specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. Hydrolysis by exo-acting enzymes such as beta-glucosidase and exo-beta-glucanase is prevented by the presence of the benzylidene group on the non-reducing end D-glucosyl residue. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture. The reaction is terminated and colour developed by the addition of a weak alkaline solution. The assay procedure is simple to use, specific, accurate, robust and readily adapted to automation |
-, 729538 |
| 3.2.1.4 | biofuel production |
bioethanol fermentation using agricultural wastes |
-, 728073 |
