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Literature summary for 3.2.1.4 extracted from

  • Malinowska, K.H.; Rind, T.; Verdorfer, T.; Gaub, H.E.; Nash, M.A.
    Quantifying synergy, thermostability, and targeting of cellulolytic enzymes and cellulosomes with polymerization-based amplification (2015), Anal. Chem., 87, 7133-7140.
    View publication on PubMed

Application

Application Comment Organism
analysis polymerization-based assay for determining the potency of cellulolytic enzyme formulations on pretreated biomass substrates by monitoring the autofluorescence of cellulose. The one-pot method is label-free, rapid, highly sensitive, and requires only a single pipetting step. Using model enzyme formulations derived from Trichoderma reesei, Trichoderma longibrachiatum, Talaromyces emersonii and recombinant bacterial minicellulosomes from Clostridium thermocellum, enzyme performance based on differences in thermostability, cellulose-binding domain targeting, and endo/exoglucanase synergy can be differentiated Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
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