EC Number   |
Application |
Reference |
|---|
   2.3.1.165 | biotechnology |
biotechnological de novo production of m-cresol from sugar in complex yeast extract-peptone medium with the yeast Saccharomyces cerevisiae. A heterologous pathway based on the decarboxylation of the polyketide 6-methylsalicylic acid is introduced into a CEN.PK yeast strain. Overexpression of codon-optimized 6-methylsalicylic acid synthase from Penicillium patulum together with activating phosphopantetheinyl transferase npgA from Aspergillus nidulans results in up to 367 mg/l 6-methylsalicylic acid production. Additional genomic integration of the genes have a strongly promoting effect and 6-methylsalicylic acid titers reach more than 2 g/l. Simultaneous expression of 6-methylsalicylic acid decarboxylase patG from Aspergillus clavatus leads to the complete conversion of 6-methylsalicylic acid and production of up to 589 mg/L m-cresol |
757577 |
| 2.3.1.165 | biotechnology |
functional heterologous expression of enzyme requires the presence of a 4-phosphoantetheinyl transferase for activation. Coexpression of both enzymes results in production of 6-methylsalicylic acid in good yields |
672715 |
| 2.3.1.165 | biotechnology |
polyketides are a group of natural products that have gained much interest due to their use as antibiotics, cholesterol lowering agents, immunosuppressors and other drugs, it is therefor of general interest to transfer polyketide synthase genes into heterologous hosts that can overproduce the corresponding polyketides |
672715 |
| 2.3.1.165 | biotechnology |
production of 6-methylsalicylic acid which is used as human pharmaceutical |
690561, 705512 |
| 2.3.1.165 | biotechnology |
production of unnatural polyketides e.g. 4-hydroxy-6-methyl-2-pyrone in E. coli and yeast after heterologous expression of the enzyme |
486452 |
| 2.3.1.165 | food industry |
the identified genes can be used as a target for PCR-based methodologies to detect the fungi responsible for producing patulin in the foodstuffs |
-, 687164 |
| 2.3.1.165 | medicine |
production of 6-methylsalicylic acid which is used as human pharmaceutical |
690561, 705512 |
| 2.3.1.165 | synthesis |
biotechnological de novo production of m-cresol from sugar in complex yeast extract-peptone medium with the yeast Saccharomyces cerevisiae. A heterologous pathway based on the decarboxylation of the polyketide 6-methylsalicylic acid is introduced into a CEN.PK yeast strain. Overexpression of codon-optimized 6-methylsalicylic acid synthase from Penicillium patulum together with activating phosphopantetheinyl transferase npgA from Aspergillus nidulans results in up to 367 mg/l 6-methylsalicylic acid production. Additional genomic integration of the genes have a strongly promoting effect and 6-methylsalicylic acid titers reach more than 2 g/l. Simultaneous expression of 6-methylsalicylic acid decarboxylase patG from Aspergillus clavatus leads to the complete conversion of 6-methylsalicylic acid and production of up to 589 mg/L m-cresol |
757577 |
| 2.3.1.165 | synthesis |
Corynebacterium glutamicum is used as a host for polyketide production. 6-Methylsalicylic acid synthase ChlB1 from Streptomyces antibioticus is introduced into this bacterium (ChlB1Sa). Challenges related to protein folding can be overcome by translation fusion of ChlB1Sa to the C-terminus of the maltose-binding protein MalE from Escherichia coli. ChlB1Sa is also active in the absence of a heterologous 4'-phosphopantetheinyl transferase, which leads to the discovery that the endogenous 4'-phosphopantetheinyl transferases PptACg of Corynebacterium glutamicum can also activate ChlB1Sa. 6-Methylsalicylic acid is an important building block in the biosynthesis of several antibiotics, including chlorothricin, maduropeptin, pactamycin, and polyketomycin |
755882 |
