Application | Comment | Organism |
---|---|---|
synthesis | Corynebacterium glutamicum is used as a host for polyketide production. 6-Methylsalicylic acid synthase ChlB1 from Streptomyces antibioticus is introduced into this bacterium (ChlB1Sa). Challenges related to protein folding can be overcome by translation fusion of ChlB1Sa to the C-terminus of the maltose-binding protein MalE from Escherichia coli. ChlB1Sa is also active in the absence of a heterologous 4'-phosphopantetheinyl transferase, which leads to the discovery that the endogenous 4'-phosphopantetheinyl transferases PptACg of Corynebacterium glutamicum can also activate ChlB1Sa. 6-Methylsalicylic acid is an important building block in the biosynthesis of several antibiotics, including chlorothricin, maduropeptin, pactamycin, and polyketomycin | Streptomyces antibioticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3 malonyl-CoA + NADPH + H+ | Streptomyces antibioticus | - |
6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces antibioticus | Q0R4P8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3 malonyl-CoA + NADPH + H+ | - |
Streptomyces antibioticus | 6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChlB1 | - |
Streptomyces antibioticus |