EC Number |
Metals/Ions |
Reference |
---|
2.7.8.13 | Mg2+ |
- |
645332, 645335 |
2.7.8.13 | Mg2+ |
absolute requirement, maximun activity at 30-40 mM |
645334 |
2.7.8.13 | Mn2+ |
can substitute for Mg2+ |
645334 |
2.7.8.13 | more |
Cu2+, Ca2+ and Zn2+ are without effect |
645334 |
2.7.8.13 | Mg2+ |
required for maximal activation, optimum concentration 25 mM |
645336 |
2.7.8.13 | Mg2+ |
can be replaced only by Mn2+ |
645340 |
2.7.8.13 | more |
no activity is observed using Ni2+, Ca2+ and Zn2+ |
645340 |
2.7.8.13 | Mg2+ |
absolute requirement for divalent cations, optimal activity with Mg2+ at concentrations 40-75 mM |
662239 |
2.7.8.13 | Mn2+ |
enzyme activity in presence of Mn2+ is 1% of the activity detected in presence of Mg2+ |
662239 |
2.7.8.13 | Mg2+ |
optimum concentration for wild-type activity: 30-60 mM, inhibitory above 100 mM, 2% of optimum activity left at 250 mM for wild-type and 30-70% for mutants (except mutant E299Q) |
690996 |