EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
5.3.2.6 | -999 |
- |
more |
4-OT exhibits hyperbolic kinetics and shows anticooperativity during catalysis |
718840 |
5.3.2.6 | 0.05 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
at pH 7.3 and 22°C |
747782 |
5.3.2.6 | 0.09 |
- |
2-oxo-4(E)-hexenedioate |
D-4OT enantiomer, pH 7.3, 30°C |
719636 |
5.3.2.6 | 0.105 |
- |
5-(methyl)-2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
5.3.2.6 | 0.14 |
- |
2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
5.3.2.6 | 0.06 |
- |
2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
5.3.2.6 | 0.211 |
- |
5-(methyl)-2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
5.3.2.6 | -999 |
- |
more |
kinetic modeling, overview |
719633 |
5.3.2.6 | 0.103 |
- |
2-oxo-4(E)-hexenedioate |
L-4OT enantiomer, pH 7.3, 30°C |
719636 |
5.3.2.6 | 0.062 |
- |
2-hydroxymuconate |
mutant 4-OT I52E, pH 7.3, 23°C |
718844 |