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Literature summary for 5.3.2.6 extracted from

  • Terrell, C.R.; Burks, E.A.; Whitman, C.P.; Hoffman, D.W.
    Structural and kinetic characterization of two 4-oxalocrotonate tautomerases in Methylibium petroleiphilum strain PM1 (2013), Arch. Biochem. Biophys., 537, 113-124.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Methylibium petroleiphilum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 200 mM tri-ammonium citrate and 10% (w/v)polyethylene glycol (PEG) 3350 Methylibium petroleiphilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
0.105
-
 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
0.14
-
 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
0.211
-
 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate Methylibium petroleiphilum
-
 (3E)-2-oxohex-3-enedioate
-
 ?

Organism

Organism UniProt Comment Textmining
Methylibium petroleiphilum A2SL37
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and SP-Sepharose column chromatography Methylibium petroleiphilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate
-
 Methylibium petroleiphilum (3E)-2-oxohex-3-enedioate
-
 ?
2-hydroxymuconate
-
 Methylibium petroleiphilum 2-oxo-3-hexenedioate
-
 ?
5-(methyl)-2-hydroxymuconate
-
 Methylibium petroleiphilum 5-(methyl)-2-oxo-3-hexenedioate
-
 ?

Synonyms

Synonyms Comment Organism
4-OT
-
 Methylibium petroleiphilum
4-oxalocrotonate tautomerase I isoform Methylibium petroleiphilum
4-oxalocrotonate tautomerase II isoform Methylibium petroleiphilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
18.5
-
 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
107
-
 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
4600
-
 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16
-
 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
310
-
 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
1000
-
 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum
33000
-
 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C Methylibium petroleiphilum