EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   3.4.21.61 | -999 |
- |
more |
analysis of binding kinetics data for PCSK9 with full-length low density lipoprotein receptor ectodomain, and isolated EGF-A repeat of low density lipoprotein receptor. The fast k-on and entropically driven thermodynamics observed for PCSK9-EGF-A binding stem from the functional replacement of water occupying stable PCSK9 hydration sites. The relatively fast k-off observed for EGF-A unbinding stems from the limited displacement of solvent occupying unstable hydration sites. Conversely, the slower k-off observed for EGF-A and low density lipoprotein receptor unbinding from mutant D374Y stems from the destabilizing effects of this mutation on PCSK9 hydration sites, with a concomitant increase in the persistence of the bound complex |
718375 |
| 3.4.21.61 | -999 |
- |
more |
binding affinities/association and dissociation constants of LDL receptor interaction with intact and cleaved enzyme, overview |
732078 |
| 3.4.21.61 | 0.001 |
- |
Ac-Nle-Tyr-Lys-Arg 4-methylcoumarin 7-amide |
pH 7.0, 37°C, in natural abundance H2O |
650054 |
| 3.4.21.61 | 0.001 |
- |
Ac-Nle-YKR 4-methylcoumarin 7-amide |
pH 7.0, 21°C |
649845 |
| 3.4.21.61 | 0.001 |
- |
D-Ac-Nle-Tyr-Lys-Arg 4-methylcoumarin 7-amide |
pH 7.0, 37°C, in natural abundance H2O |
650054 |
| 3.4.21.61 | 0.023 |
- |
benzyloxycarbonyl-Ala-Tyr-Lys-Lys 4-methylcoumarin 7-amide |
pH 7.0, 21°C |
649306 |
| 3.4.21.61 | 0.038 |
- |
Ac-Nle-YKK 4-methylcoumarin 7-amide |
pH 7.0, 21°C |
649845 |
| 3.4.21.61 | 0.32 |
- |
t-butyloxycarbonyl-QGR 4-methylcoumarin 7-amide |
pH 7.0, 21°C |
649845 |
| 3.4.21.61 | 0.35 |
- |
Ac-AYKK 4-methylcoumarin 7-amide |
pH 7.0, 21°C |
649845 |
