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Literature summary for 3.4.21.61 extracted from

  • Rockwell, N.C.; Fuller, R.S.
    Direct measurement of acylenzyme hydrolysis demonstrates rate-limiting deacylation in cleavage of physiological sequences by the processing protease Kex2 (2001), Biochemistry, 40, 3657-3665.
    View publication on PubMed
Search for more literature for 3.4.21.61

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 D-Ac-Nle-Tyr-Lys-Arg 4-methylcoumarin 7-amide pH 7.0, 37°C, in natural abundance H2O Saccharomyces cerevisiae
0.001
-
 Ac-Nle-Tyr-Lys-Arg 4-methylcoumarin 7-amide pH 7.0, 37°C, in natural abundance H2O Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus trans Golgi network Saccharomyces cerevisiae 5794
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-alpha-mating factor + H2O Saccharomyces cerevisiae mating pheromone precursor, physiological substrate alpha-mating factor + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 yeast
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-Nle-Tyr-Lys-Arg 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + Ac-Nle-Tyr-Lys-Arg-COOH
-
 ?
Ac-Pro-Met-Tyr-Lys-Arg 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae 7-amino-4-methylcoumarin + ?
-
 ?
Arg-Lys(DABCYL)-Nle-Tyr-Lys-Arg-Glu-Ala-Glu-Ala-Glu(EDANS)-Arg + H2O
-
 Saccharomyces cerevisiae Arg-Lys(DABCYL)-Nle-Tyr-Lys-Arg + Glu-Ala-Glu-Ala-Glu(EDANS)-Arg
-
 ?
Arg-Lys(DABCYL)-Nle-Tyr-Lys-Lys-Glu-Ala-Glu-Ala-Glu(EDANS)-Arg + H2O
-
 Saccharomyces cerevisiae Arg-Lys(DABCYL)-Nle-Tyr-Lys-Lys + Glu-Ala-Glu-Ala-Glu(EDANS)-Arg
-
 ?
D-Ac-Nle-Tyr-Lys-Arg 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
pro-alpha-mating factor + H2O
-
 Saccharomyces cerevisiae alpha-mating factor + ?
-
 ?
pro-alpha-mating factor + H2O mating pheromone precursor, physiological substrate Saccharomyces cerevisiae alpha-mating factor + ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2700
-
 Ac-Nle-Tyr-Lys-Arg 4-methylcoumarin 7-amide pH 7.0, 37°C Saccharomyces cerevisiae
2940
-
 D-Ac-Nle-Tyr-Lys-Arg 4-methylcoumarin 7-amide pH 7.0, 37°C Saccharomyces cerevisiae