EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.7.1.19 | 0.1 |
- |
ATP |
- |
641031 |
2.7.1.19 | 0.29 |
0.33 |
D-ribulose 5-phosphate |
- |
641031 |
2.7.1.19 | 0.24 |
- |
D-ribulose 5-phosphate |
30°C |
641030 |
2.7.1.19 | 0.71 |
- |
ATP |
30°C |
641030 |
2.7.1.19 | 11 |
- |
D-ribulose 5-phosphate |
K165C mutant, pH 8, 30°C |
641049 |
2.7.1.19 | 10.3 |
- |
D-ribulose 5-phosphate |
K165M mutant, pH 8, 30°C |
641049 |
2.7.1.19 | -999 |
- |
more |
kinetics |
641029 |
2.7.1.19 | 0.78 |
- |
D-ribulose 5-phosphate |
mutant enzyme H100A, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate |
677023 |
2.7.1.19 | 1.37 |
- |
D-ribulose 5-phosphate |
mutant enzyme H100N, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate |
677023 |
2.7.1.19 | 1.35 |
- |
D-ribulose 5-phosphate |
mutant enzyme H100Q, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate |
677023 |