Literature summary for 2.7.1.19 extracted from

  • Runquist, J.A.; Harrison, D.H.T.; Miziorko, H.M.
    Rhodobacter sphaeroides phosphoribulokinase: identification of lysine-165 as a catalytic residue and evaluation of the contributions of invariant basic amino acids to ribulose 5-phosphate binding (1999), Biochemistry, 38, 13999-14005.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutants Cereibacter sphaeroides

Engineering

Protein Variants Comment Organism
K165M reduced catalytic activity, insensitive to inactivation by pyridoxal 5'-phosphate Cereibacter sphaeroides
K165C reduced catalytic activity Cereibacter sphaeroides
K53M sensitive to inactivation by pyridoxal 5'-phosphate Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
6-phospho-D-gluconate competitive with respect to D-ribulose 5-phosphate Cereibacter sphaeroides
pyridoxal 5'-phosphate inactivation is protected by ATP in combination with NADH Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.096
-
D-ribulose 5-phosphate wild-type enzyme, pH 8, 30°C Cereibacter sphaeroides
11
-
D-ribulose 5-phosphate K165C mutant, pH 8, 30°C Cereibacter sphaeroides
10.3
-
D-ribulose 5-phosphate K165M mutant, pH 8, 30°C Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate + ATP
-
Cereibacter sphaeroides D-ribulose 1,5-diphosphate + ADP
-
?
D-ribulose 5-phosphate + trinitrophenyl-ATP spectroscopically active ATP-analog Cereibacter sphaeroides D-ribulose 1,5-diphosphate + trinitrophenyl-ADP
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Cereibacter sphaeroides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
11
-
6-phospho-D-gluconate K165M mutant, pH 8, 30°C Cereibacter sphaeroides
1.15
-
6-phospho-D-gluconate R173Q mutant, pH 8, 30°C Cereibacter sphaeroides
0.222
-
6-phospho-D-gluconate wild-type enzyme, pH 8, 30°C Cereibacter sphaeroides
0.415
-
6-phospho-D-gluconate R168Q mutant, pH 8, 30°C Cereibacter sphaeroides
2.81
-
6-phospho-D-gluconate H45N mutant, pH 8, 30°C Cereibacter sphaeroides
19.8
-
6-phospho-D-gluconate R49Q mutant, pH 8, 30°C Cereibacter sphaeroides