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Literature summary for 2.7.1.19 extracted from

  • Runquist, J.A.; Miziorko, H.M.
    Functional contribution of a conserved, mobile loop histidine of phosphoribulokinase (2006), Protein Sci., 15, 837-842.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
H100A reduced activity Cereibacter sphaeroides
H100N reduced activity Cereibacter sphaeroides
H100Q reduced activity Cereibacter sphaeroides
H45N similar to wild type enzyme Cereibacter sphaeroides
Y98L reduced activity Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
6-phosphogluconate competitive inhibitor, 78% residual activity at 1 mM Cereibacter sphaeroides
diethyl dicarbonate 27% residual activity at 0.04 mM Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
D-ribulose 5-phosphate wild type enzyme, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate Cereibacter sphaeroides
0.78
-
D-ribulose 5-phosphate mutant enzyme H100A, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate Cereibacter sphaeroides
1.35
-
D-ribulose 5-phosphate mutant enzyme H100Q, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate Cereibacter sphaeroides
1.37
-
D-ribulose 5-phosphate mutant enzyme H100N, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate Cereibacter sphaeroides
4
-
D-ribulose 5-phosphate mutant enzyme Y98L, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose chromatography Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate + ATP
-
Cereibacter sphaeroides D-ribulose 1,5-diphosphate + ADP
-
?

Synonyms

Synonyms Comment Organism
Prk
-
Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
ATP
-
Cereibacter sphaeroides