EC Number   |
KM Value [mM] |
KM Value Maximum [mM]  |
Substrate |
Reference |
|---|
   3.4.22.51 | 0.019 |
- |
2-aminobenzoyl-ARF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH |
37°C, pH 5.5 |
668458 |
| 3.4.22.51 | 0.028 |
- |
azocasein |
pH 4.5, 25°C |
638888 |
| 3.4.22.51 | 0.028 |
- |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin |
pH 5.0, 25°C, wild-type enzyme |
752781 |
| 3.4.22.51 | 0.029 |
- |
azocasein |
pH 8.5, 40°C |
638888 |
| 3.4.22.51 | 0.033 |
- |
N-carbobenzoxy-VVR-4-nitroanilide |
pH 9 |
638877, 638879 |
| 3.4.22.51 | 0.038 |
- |
benzyloxycarbonyl-Arg-Ala-7-amido-4-methylcoumarin |
pH 7.5, 25°C, wild-type enzyme |
752781 |
| 3.4.22.51 | 0.043 |
- |
benzoyl-His-Arg-7-amido-4-methylcoumarin |
pH 6.3, 37°C |
638876 |
| 3.4.22.51 | 0.045 |
- |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin |
pH 7.5, 25°C, mutant enzyme E208A |
752781 |
| 3.4.22.51 | 0.056 |
- |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin |
pH 4.4, 25°C, wild-type enzyme |
752781 |
| 3.4.22.51 | 0.066 |
- |
benzoyl-PFR-4-nitroanilide |
- |
638877, 638879 |
