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Information on EC 3.4.22.51 - cruzipain
for references in articles please use BRENDA:EC3.4.22.51
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EC Tree 3 Hydrolases
3.4 Acting on peptide bonds (peptidases)
3.4.22 Cysteine endopeptidases
3.4.22.51 cruzipain
IUBMB Comments
In peptidase family C1. Is located in the digestive vacuoles of the parasitic trypanosome and contributes to the nutrition of the organism by digestion of host proteins.
The enzyme appears in viruses and cellular organisms
- 3.4.22.51
- cathepsins
- brucei
-
protozoan
- epimastigotes
- trypomastigotes
-
trypanocidal
- trypanosomiasis
-
trypanosomal
- papain
- proteinases
- amastigote
-
papain-like
- benznidazole
-
anti-trypanosoma
-
antiparasitic
-
latin
- thiosemicarbazone
-
vinyl
- rhodesiense
- cystatins
-
nitril
- trypanothione
- nifurtimox
-
antichagasic
- chagasin
- bauhinia
- kininogens
-
warhead
-
metacyclic
- medicine
-
metacyclogenesis
- falcipain-2
- congolense
-
cruzi-infected
- trans-sialidase
-
tulahuen
-
reservosomes
-
n-acylhydrazones
- pharmacology
- molecular biology
- z-phe-arg-amc
- semicarbazone
- drug development
showSynonyms
cruzipain, cruzain, rhodesain, major cysteine proteinase, congopain, cathepsin l-like cysteine protease, cathepsin l-like protease, gp57/51, brucipain, trypanopain, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
brucipain
cathepsin L-like cysteine protease
CatL-like protease
congopain
CP
cruzain
cruzipain
CZP
evansain
-
-
-
-
GP57/51
-
-
-
-
major cysteine proteinase
proteinase, Trypanosoma congolese cysteine
-
-
-
-
proteinase, Trypanosoma cruzi cysteine
-
-
-
-
proteinase, Trypanosoma cysteine
-
-
-
-
trypanopain
-
-
-
-
Trypanosoma congolese cysteine protease
-
-
-
-
Trypanosoma cruzi cysteine protease
-
-
-
-
Trypanosoma cysteine protease
-
-
-
-
CatL-like protease
-
congopain
-
-
-
-
cruzain
-
-
-
-
cruzipain
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
broad endopeptidase specificity similar to that of cathepsin L
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
hydrolysis of peptide bond
-
-
-
-
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CAS REGISTRY NUMBER
COMMENTARY
102227-51-0
-
141588-22-9
-
84399-99-5
-
90371-53-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(7-[[(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(morpholin-4-ylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(morpholin-4-ylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(naphthalen-2-ylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(naphthalen-2-ylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(phenylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(phenylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(quinolin-6-ylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(quinolin-6-ylcarbonyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(quinolin-6-ylmethyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1,2-dimethyl-1-[(quinolin-6-ylmethyl)amino]propyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-(4-[(1S)-1-[(1,3-benzothiazol-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1-[(1,3-benzothiazol-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-(4-[(1S)-1-[(1,3-benzothiazol-6-ylmethyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1-[(1,3-benzothiazol-6-ylmethyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-(4-[(1S)-1-[(1-benzofuran-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1-[(1-benzofuran-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-(4-[(1S)-1-[(1-benzothiophen-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1-[(1-benzothiophen-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-(4-[(1S)-1-[(1H-benzotriazol-5-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1-[(1H-benzotriazol-5-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-(4-[(1S)-1-[(1H-indol-5-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1-[(1H-indol-5-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-(4-[(1S)-1-[(1H-indol-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1-[(1H-indol-6-ylcarbonyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-(4-[(1S)-1-[(4-methoxybenzyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-(4-[(1S)-1-[(4-methoxybenzyl)amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl)hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(1-methyl-1H-indol-6-yl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(1-methyl-1H-indol-6-yl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(2-methylphenyl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(2-methylphenyl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(3-methylphenyl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(3-methylphenyl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(4-methylphenyl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-[4-[(1S)-1,2-dimethyl-1-[[(4-methylphenyl)carbonyl]amino]propyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-[4-[(1S)-1-(benzylamino)-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-[4-[(1S)-1-(benzylamino)-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-[4-[(1S)-1-[[(2-methoxyphenyl)carbonyl]amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-[4-[(1S)-1-[[(2-methoxyphenyl)carbonyl]amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-[4-[(1S)-1-[[(3-methoxyphenyl)carbonyl]amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-[4-[(1S)-1-[[(3-methoxyphenyl)carbonyl]amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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(7-[[(2S)-2-[4-[(1S)-1-[[(4-methoxyphenyl)carbonyl]amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoyl]amino]-4-methyl-2-oxo-3,4-dihydro-2H-chromen-3-yl)acetic acid + H2O
(2S)-2-[4-[(1S)-1-[[(4-methoxyphenyl)carbonyl]amino]-1,2-dimethylpropyl]-1H-1,2,3-triazol-1-yl]hexanoic acid + 7-amino-4-methylcoumarin-3-acetic acid
Substrates: -
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2-aminobenzoic acid-ALRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
Substrates: -
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?
2-aminobenzoic acid-KLGFSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
Substrates: -
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?
2-aminobenzoic acid-KLRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
Substrates: and analogues
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2-aminobenzoic acid-peptidyl-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
Substrates: and derivatives, enzyme shows preference for benzyl-Cys or Arg at the P1 position and a hydrophobic non-aromatic residue at position P2
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?
2-aminobenzoyl-ARF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH + H2O
?
-
Substrates: -
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?
2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-NH2 + H2O
?
-
Substrates: -
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?
2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH + H2O
?
-
Substrates: -
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?
2-aminobenzoyl-FRF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH + H2O
?
-
Substrates: -
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?
2-aminobenzoyl-Phe-Arg-(2,4-dinitrophenyl)-epsilon-NH2-lysine-Pro-OH + H2O
?
-
Substrates: -
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?
2-aminobenzoyl-Phe-Arg-(2,4-dinitrophenyl)-epsilon-NH2-lysine-Trp-OH + H2O
?
-
Substrates: -
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?
2-aminobenzoyl-RRF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH + H2O
?
-
Substrates: -
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Ac-Phe-Arg-7-amido-4-methylcoumarin + H2O
Ac-Phe-Arg + 7-amino-4-methylcoumarin
-
Substrates: 10 microM, 4 nanoM cruzain, 45 min, room temperature, pH 5.5
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?
benzoyl-His-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-His-Arg + 7-amino-4-methylcoumarin
Substrates: -
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?
benzoyl-HR-4-methylcoumarin 7-amide + H2O
?
Substrates: less susceptible substrate
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?
benzoyl-Phe-Arg-4-methylcoumarin-7-amide + H2O
?
Substrates: fluorogenic substrates based on the peptide benzoyl-Phe-Arg-4-methylcoumarin-7-amide
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?
benzoyl-Phe-Val-Arg-p-nitroanilide + H2O
?
-
Substrates: as active as with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide
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?
benzoyl-Pro-Phe-Arg-p-nitroanilide + H2O
?
-
Substrates: 41% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide
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?
benzoyl-RR-4-methylcoumarin 7-amide + H2O
?
Substrates: less susceptible substrate
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benzoyl-Tyr-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Tyr-Arg + 7-amino-4-methylcoumarin
Substrates: -
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?
benzoyl-Val-Gly-Arg-p-nitroanilide + H2O
?
-
Substrates: 52% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide
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?
benzoyl-YR-4-methylcoumarin 7-amide + H2O
?
Substrates: -
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?
benzyloxycarbonyl-Ala-Arg-Arg-methoxy-beta-naphthylamide + H2O
?
-
Substrates: 36% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide
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benzyloxycarbonyl-Arg-Ala-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Ala + 7-amino-4-methylcoumarin
Substrates: -
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?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
Substrates: -
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?
benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide + H2O
?
-
Substrates: -
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?
benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide + H2O
?
-
Substrates: 66% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide
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benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
Substrates: relatively low rates of substrate hydrolysis
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benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
benzyloxycarbonyl-Phe-Arg-methoxy-beta-naphthylamide + H2O
?
-
Substrates: 30% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide
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Bz-Phe-Arg-4-methylcoumaryl-7-amide + H2O
Bz-Phe-Arg + 7-amino-4-methylcoumarin
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Substrates: -
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Bz-Pro-Phe-Arg-4-nitroanilide + H2O
Bz-Pro-Phe-Arg + 4-nitroaniline
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Substrates: -
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?
epsilon-NH2(capryl)-L-(SBzl)C-7-amido-4-methylcoumarin + H2O
?
-
Substrates: -
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human IgG + H2O
?
-
Substrates: specific cleavage sites on human IgG subclasses by cruzipain
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Lys-Arg-primaquine + H2O
Lys-Arg + primaquine
-
Substrates: -
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N-alpha-benzyloxy-carbonyl-L-phenylalanyl-L-alanine-(7-amido-4-methylcoumarin) + H2O
N-alpha-benzyloxy-carbonyl-L-phenylalanyl-L-alanine + 7-amino-4-methylcoumarin
-
Substrates: -
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N-alpha-benzyloxycarbonyl-L-arginyl-L-alanine-(7-amido-4-methylcoumarin) + H2O
N-alpha-benzyloxycarbonyl-L-arginyl-L-alanine + 7-amino-4-methylcoumarin
-
Substrates: -
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N-alpha-benzyloxycarbonyl-L-tyrosyl-L-alanine-(7-amido-4-methylcoumarin) + H2O
N-alpha-benzyloxycarbonyl-L-tyrosyl-L-alanine + 7-amino-4-methylcoumarin
-
Substrates: -
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N-carbobenzoxy-Arg-Arg-2-(4-methoxy)-naphthylamide + H2O
N-carbobenzoxy-Arg-Arg + 2-amino-4-methoxynaphthalene
Substrates: -
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?
N-tert-butoxycarbonyl-O-benzyl-Ser-Gly-Arg-p-nitroanilide + H2O
?
-
Substrates: 39% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide
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N-tert-butoxycarbonyl-Val-Leu-Gly-Arg-p-nitroanilide + H2O
?
-
Substrates: 24% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide
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o-aminobenzoyl-ALRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
-
Substrates: -
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o-aminobenzoyl-HLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
-
Substrates: -
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o-aminobenzoyl-KARFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
-
Substrates: -
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o-aminobenzoyl-KFRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
-
Substrates: -
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o-aminobenzoyl-KLFFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KLF + FSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
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Substrates: -
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o-aminobenzoyl-KLGFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KLG + FSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
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Substrates: -
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o-aminobenzoyl-KLKFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KLK + FSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
Substrates: -
Products: -
Products: -
?
o-aminobenzoyl-KLRFAKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KLR + FAKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
Substrates: -
Products: -
Products: -
?
o-aminobenzoyl-KLRFFKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KLR + FFKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
Substrates: -
Products: -
Products: -
?
o-aminobenzoyl-KLRFPKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KLR + FPKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
Substrates: -
Products: -
Products: -
?
o-aminobenzoyl-KLRFSFQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KLR + FSFQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
Substrates: no hydrolysis is detected
Products: -
Products: -
?
o-aminobenzoyl-KLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KLR + FSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
Substrates: -
Products: -
Products: -
?
o-aminobenzoyl-KLRFSPQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KLR + FSPQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
Substrates: -
Products: -
Products: -
?
o-aminobenzoyl-KLRFSRQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KLR + FSRQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
Substrates: -
Products: -
Products: -
?
o-aminobenzoyl-KLRLSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KLR + LSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
Substrates: -
Products: -
Products: -
?
o-aminobenzoyl-KLRRSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KLR + RSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
Substrates: -
Products: -
Products: -
?
o-aminobenzoyl-KPRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
-
Substrates: -
Products: -
Products: -
?
o-aminobenzoyl-KRRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
-
Substrates: -
Products: -
Products: -
?
o-aminobenzoyl-LLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
-
Substrates: -
Products: -
Products: -
?
peptidyl-4-nitroanilide + H2O
?
Substrates: derivatives of
Products: -
Products: -
?
peptidyl-methylcoumarin amide + H2O
?
Substrates: and derivatives, enzyme shows preference for benzyl-Cys or Arg at the P1 position and a hydrophobic non-aromatic residue at position P2
Products: -
Products: -
?
Phe-Ala-primaquine + H2O
Phe-Ala + primaquine
-
Substrates: -
Products: -
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?
Phe-Arg-primaquine + H2O
Phe-Arg + primaquine
-
Substrates: -
Products: -
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?
tosyl-Gly-Pro-Arg-p-nitroanilide + H2O
tosyl-Gly-Pro-Arg + p-nitroaniline
-
Substrates: 25% of the activity with benzyloxycarbonyl-Arg-Arg-methoxy-beta-naphthylamide
Products: -
Products: -
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
-
Substrates: -
Products: -
Products: -
?
2-aminobenzoyl-LGMISLMKRPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
Substrates: -
Products: -
Products: -
?
2-aminobenzoyl-LGMISLMKRPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
Substrates: Abz-LGMISLMKRPQ-EDDnp
Products: -
Products: -
?
benzoyl-FR-4-methylcoumarin 7-amide + H2O
?
Substrates: -
Products: -
Products: -
?
benzoyl-FR-4-methylcoumarin 7-amide + H2O
?
Substrates: fluorigenic substrates based on this peptide, substitution of Phe results in less susceptible substrates
Products: -
Products: -
?
benzoyl-PFR-4-nitroanilide + H2O
?
Substrates: -
Products: -
Products: -
?
benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Phe-Arg + 7-amino-4-methylcoumarin
Substrates: -
Products: -
Products: -
?
benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Phe-Arg + 7-amino-4-methylcoumarin
-
Substrates: -
Products: -
Products: -
?
benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Phe-Arg + 7-amino-4-methylcoumarin
Substrates: -
Products: -
Products: -
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
?
Substrates: -
Products: -
Products: -
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
?
Substrates: -
Products: -
Products: -
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
?
-
Substrates: -
Products: -
Products: -
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
?
Substrates: -
Products: -
Products: -
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
?
-
Substrates: -
Products: -
Products: -
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
?
Substrates: -
Products: -
Products: -
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
Substrates: -
Products: -
Products: -
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
Substrates: -
Products: -
Products: -
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
Substrates: -
Products: -
Products: -
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
Substrates: -
Products: -
Products: -
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
Trypanosoma cruzi INC-5
Substrates: -
Products: -
Products: -
?
Gelatin + H2O
?
Substrates: -
Products: -
Products: -
?
Immunoglobulin G1 + H2O
?
Substrates: cleaves specifically at the hinge region
Products: -
Products: -
?
Immunoglobulin G3 + H2O
?
Substrates: cleaves specifically at the hinge region
Products: -
Products: -
?
N-carbobenzoxy-Val-Val-Arg-4-nitroanilide + H2O
?
Substrates: -
Products: -
Products: -
?
N-carbobenzoxy-Val-Val-Arg-4-nitroanilide + H2O
?
Substrates: excellent substrate
Products: -
Products: -
?
N-carbobenzoxy-VVR-4-nitroanilide + H2O
N-carbobenzoxy-VVR + 4-nitroaniline
Substrates: -
Products: -
Products: -
?
N-carbobenzoxy-VVR-4-nitroanilide + H2O
N-carbobenzoxy-VVR + 4-nitroaniline
-
Substrates: -
Products: -
Products: -
?
peptidyl-beta-naphthylamide + H2O
?
Substrates: derivatives of
Products: -
Products: -
?
peptidyl-beta-naphthylamide + H2O
?
-
Substrates: derivatives of
Products: -
Products: -
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
?
-
Substrates: -
Products: -
Products: -
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
?
-
Substrates: 5 microM, pH 6.5, 28°C
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme may be involved in facilitating parasite entry into the brain
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme may be involved in facilitating parasite entry into the brain
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: benzyloxycarbonyl-Arg-Arg-aminomethyl coumarin
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrate: benzyloxycarbonyl-Arg-Arg-aminomethyl coumarin
Products: -
Products: -
?
additional information
?
-
Substrates: substrates are chromogenic peptides with a carboxyl Arg or Lys, enzyme requires at least one more amino acid, preferably Arg, Phe, Val or Leu between the terminal Arg or Lys and the amino-blocking group
Products: -
Products: -
?
additional information
?
-
Substrates: substrates are chromogenic peptides with a carboxyl Arg or Lys, enzyme requires at least one more amino acid, preferably Arg, Phe, Val or Leu between the terminal Arg or Lys and the amino-blocking group
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrates are chromogenic peptides with a carboxyl Arg or Lys, enzyme requires at least one more amino acid, preferably Arg, Phe, Val or Leu between the terminal Arg or Lys and the amino-blocking group
Products: -
Products: -
?
additional information
?
-
Substrates: synthetic chromogenic sustrates
Products: -
Products: -
?
additional information
?
-
-
Substrates: synthetic chromogenic sustrates
Products: -
Products: -
?
additional information
?
-
Substrates: antigenic character in natural human infections
Products: -
Products: -
?
additional information
?
-
-
Substrates: antigenic character in natural human infections
Products: -
Products: -
?
additional information
?
-
Substrates: stimulates potent humoral and cellular immune response during infection in both humans and mice
Products: -
Products: -
?
additional information
?
-
-
Substrates: stimulates potent humoral and cellular immune response during infection in both humans and mice
Products: -
Products: -
?
additional information
?
-
Substrates: enzyme plays a role in the production of antibodies against cardiac M2 muscarinic acetylcholine receptor
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme plays a role in the production of antibodies against cardiac M2 muscarinic acetylcholine receptor
Products: -
Products: -
?
additional information
?
-
-
Substrates: cruzipain may act as a survival factor for cardiomyocytes because it rescues them from apoptosis and stimulates arginase-2
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity of cruzipain 2 isoform is analysed using internally quenched flurogenic substrates. It is found that cruzipain 2 compared to cruzipain differs substantially regarding the specificity in the S2, S1' and S2' pockets, suggesting that cruzipain 2 acts on distinct natural substrates
Products: -
Products: -
?
additional information
?
-
-
Substrates: design of selective prodrugs of primaquine: Lys-Arg-primaquine, Phe-Ala-primaquine, and Phe-Arg-primaquine, cleaved by the enzyme in vivo and selectively inhibiting it, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: design of selective prodrugs of primaquine: Lys-Arg-primaquine, Phe-Ala-primaquine, and Phe-Arg-primaquine, cleaved by the enzyme in vivo and selectively inhibiting it, overview. The molecular mechanism acts via nucleophilic attack of the catalytic Cys25 residue on the respective prodrug amide carbonyl carbon, releasing primaquine, docking study showing specific interactions between the prodrug and the S1 and S2 pockets of cruzipain with the dipeptides acting as distance holders
Products: -
Products: -
?
additional information
?
-
-
Substrates: involvement of O-linked GlcNAc moiety present in the major cysteine protease both in cruzipain recognition and in the cross-reactivity between cruzipain and myosin molecules
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Gelatin + H2O
?
Substrates: -
Products: -
Products: -
?
Lys-Arg-primaquine + H2O
Lys-Arg + primaquine
-
Substrates: -
Products: -
Products: -
?
Phe-Ala-primaquine + H2O
Phe-Ala + primaquine
-
Substrates: -
Products: -
Products: -
?
Phe-Arg-primaquine + H2O
Phe-Arg + primaquine
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme may be involved in facilitating parasite entry into the brain
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme may be involved in facilitating parasite entry into the brain
Products: -
Products: -
?
additional information
?
-
Substrates: antigenic character in natural human infections
Products: -
Products: -
?
additional information
?
-
-
Substrates: antigenic character in natural human infections
Products: -
Products: -
?
additional information
?
-
Substrates: stimulates potent humoral and cellular immune response during infection in both humans and mice
Products: -
Products: -
?