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Literature summary for 3.4.22.51 extracted from

  • Alves, L.C.; Melo, R.L.; Cezari, M.H.S.; Sanderson, S.J.; Mottram, J.C.; Coombs, G.H.; Juliano, L.; Juliano, M.A.
    Analysis of the S2 subsite specificities of the recombinant cysteine proteinases CPB of Leishmania mexicana, and cruzain of Trypanosoma cruzi, using fluorescent substrates containing non-natural basic amino acids (2001), Mol. Biochem. Parasitol., 117, 137-143.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 benzyloxycarbonyl-FR-aminomethyl coumarin pH 6.3, 37°C Trypanosoma cruzi
0.0012
-
 benzoyl-Phe-Arg-7-amido-4-methylcoumarin pH 6.3, 37°C Trypanosoma cruzi
0.0119
-
 benzoyl-RR-aminomethyl coumarin pH 6.3, 37°C Trypanosoma cruzi
0.043
-
 benzoyl-His-Arg-7-amido-4-methylcoumarin pH 6.3, 37°C Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q598P5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-FR-4-methylcoumarin 7-amide + H2O fluorigenic substrates based on this peptide, substitution of Phe results in less susceptible substrates Trypanosoma cruzi ?
-
 ?
benzoyl-His-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma cruzi benzoyl-His-Arg + 7-amino-4-methylcoumarin
-
 ?
benzoyl-HR-4-methylcoumarin 7-amide + H2O less susceptible substrate Trypanosoma cruzi ?
-
 ?
benzoyl-Phe-Arg-4-methylcoumarin-7-amide + H2O fluorogenic substrates based on the peptide benzoyl-Phe-Arg-4-methylcoumarin-7-amide Trypanosoma cruzi ?
-
 ?
benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma cruzi benzoyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
benzoyl-RR-4-methylcoumarin 7-amide + H2O less susceptible substrate Trypanosoma cruzi ?
-
 ?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Trypanosoma cruzi ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0783
-
 benzoyl-RR-aminomethyl coumarin
-
 Trypanosoma cruzi
0.158
-
 benzyloxycarbonyl-FR-aminomethyl coumarin
-
 Trypanosoma cruzi
0.172
-
 benzoyl-His-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma cruzi
0.193
-
 benzoyl-Phe-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma cruzi