EC Number |
General Stability |
Reference |
---|
5.1.1.1 | 5 min, about 50% loss of activity after incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide |
2033 |
5.1.1.1 | 5 min, resistant to incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide |
2033 |
5.1.1.1 | acetate increases the stability of enzyme ALR, enzyme residue A131 mediates the activation and stabilization of enzyme ALR by short chain carboxylates |
749379 |
5.1.1.1 | denatured by 3.5 M urea in one transition phase |
2033 |
5.1.1.1 | denatured by urea in two transition phases, dissociation of pyridoxal 5'-phosphate with 4.0 M urea, unfolding with 5.5 M urea |
2033 |
5.1.1.1 | in 0.6 M to 1.5 M guanidine hydrochloride the dimeric enzyme is dissociated into a monomeric form, which is catalytically inactive |
2025, 2029 |
5.1.1.1 | NaB3H4-reduced DadB holoenzyme is resistant to alpha-chymotrypsin and trypsin and is labile only towards subtilisin |
2039 |
5.1.1.1 | pyridoxal 5'-phosphate present in the solvent suppresses the enzyme's unfolding |
651357 |
5.1.1.1 | stabilized in presence of monovalent anions including Cl- |
652947 |
5.1.1.1 | stable in 30% ammonium sulfate. Irreversibly diminished activity by exposure to ammonium sulfate concentrations near or above 40%, where precipitation occurs |
2013 |