Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | wild-type enzyme ALR is strongly activated by low concentrations (e.g. 1 mM) of short-chain carboxylates, and is inhibited at higher concentrations (e.g. 10 mM), enzyme residue A131 mediates the activation and stabilization of enzyme ALR by short chain carboxylates | Ligilactobacillus salivarius |
Cloned (Comment) | Organism |
---|---|
gene alr, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-CodonPlusTM(DE3)-RIPL | Ligilactobacillus salivarius |
Protein Variants | Comment | Organism |
---|---|---|
A131K | site-directed mutagenesis, the mutant shows a 3fold increased activity compared to wild-type | Ligilactobacillus salivarius |
General Stability | Organism |
---|---|
acetate increases the stability of enzyme ALR, enzyme residue A131 mediates the activation and stabilization of enzyme ALR by short chain carboxylates | Ligilactobacillus salivarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Ligilactobacillus salivarius | |
acetate | - |
Ligilactobacillus salivarius | |
aspartate | - |
Ligilactobacillus salivarius | |
Butyrate | - |
Ligilactobacillus salivarius | |
DL-lactate | - |
Ligilactobacillus salivarius | |
additional information | wild-type enzyme ALR is strongly activated by low concentrations (e.g. 1 mM) of short-chain carboxylates, and is inhibited at higher concentrations (e.g. 10 mM). The enzyme mutant ALRA131K is inhibited at all carboxylate concentrations tested (1-40 mM). Both propionate and butyrate strongly inhibit mutant ALRA131K. ALR and ALRA131K are both inhibited by DL-lactate, though wild-type ALR is inhibited to a lesser degree than ALRA131K. In addition, succinate, pyruvate, 2-oxoglutarate, oxaloacetate, and aspartate also more strongly inhibit mutant ALRA131K than wild-type ALR | Ligilactobacillus salivarius | |
oxaloacetate | - |
Ligilactobacillus salivarius | |
propionate | - |
Ligilactobacillus salivarius | |
pyruvate | - |
Ligilactobacillus salivarius | |
succinate | - |
Ligilactobacillus salivarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | Ligilactobacillus salivarius | - |
D-alanine | - |
r | |
L-alanine | Ligilactobacillus salivarius UCC 118 | - |
D-alanine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ligilactobacillus salivarius | Q1WV14 | - |
- |
Ligilactobacillus salivarius UCC 118 | Q1WV14 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlusTM(DE3)-RIPL by nickel affinity chromatography, dialysis, and ultrafiltration | Ligilactobacillus salivarius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
161 | - |
purified recombinant His-tagged wild-type enzyme, pH 7.0, 30°C | Ligilactobacillus salivarius |
438 | - |
purified recombinant His-tagged mutant A131K enzyme, pH 7.0, 30°C | Ligilactobacillus salivarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | - |
Ligilactobacillus salivarius | D-alanine | - |
r | |
L-alanine | - |
Ligilactobacillus salivarius UCC 118 | D-alanine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Ligilactobacillus salivarius |
Synonyms | Comment | Organism |
---|---|---|
ALR | - |
Ligilactobacillus salivarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
recombinant wild-type enzyme, without acetate | Ligilactobacillus salivarius |
45 | - |
recombinant enzyme mutant A131K, with or without acetate | Ligilactobacillus salivarius |
50 | - |
recombinant wild-type enzyme, in presence of 1 mM acetate | Ligilactobacillus salivarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant wild-type enzyme, with or without acetate | Ligilactobacillus salivarius |
9 | - |
recombinant enzyme mutant A131K, with or without acetate | Ligilactobacillus salivarius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Ligilactobacillus salivarius |
General Information | Comment | Organism |
---|---|---|
physiological function | alanine racemase (ALR) is responsible for the high D-alanine production in Lactobacillus salivarius | Ligilactobacillus salivarius |