General Stability | Organism |
---|---|
5 min, about 50% loss of activity after incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide | Pseudomonas fluorescens |
5 min, resistant to incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide | Geobacillus stearothermophilus |
denatured by 3.5 M urea in one transition phase | Geobacillus stearothermophilus |
denatured by urea in two transition phases, dissociation of pyridoxal 5'-phosphate with 4.0 M urea, unfolding with 5.5 M urea | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values of L-Ala in the presence of urea at various concentrations | Geobacillus stearothermophilus | |
additional information | - |
additional information | Km values of L-Ala in the presence of urea at various concentrations | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | - |
Geobacillus stearothermophilus | D-Ala | - |
? | |
L-Ala | - |
Pseudomonas fluorescens | D-Ala | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
over 30°C, 1 h, quick loss of activity | Pseudomonas fluorescens |
30 | - |
5 min, about 50% loss of activity after incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide | Pseudomonas fluorescens |
30 | - |
5 min, resistant to incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide | Geobacillus stearothermophilus |
75 | - |
1 h, inactivation over 75°C | Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.3 | 10.5 | 1 h, 0°C, stable | Geobacillus stearothermophilus |
8.3 | 10.5 | 1 h, 0°C, stable | Pseudomonas fluorescens |