EC Number |
General Stability |
Reference |
---|
2.6.1.7 | alcohol fractionation inactivates |
636606 |
2.6.1.7 | ammonium sulfate fractionation inactivates |
636606 |
2.6.1.7 | dialysis against phosphate buffer inactivates, 2-oxoglutarate protects to some extent |
636606 |
2.6.1.7 | dialysis of the apoenzyme against Tris-HCl or imidazole-HCl buffer inactivates |
636608 |
2.6.1.7 | freeze-thawing inactivates |
636606 |
2.6.1.7 | repeated freeze-thawing inactivates enzyme irriversible in dilute solution, below 0.1 mg/ml |
636613 |
2.6.1.7 | slightly acidic buffer solutions inactivate, pyridoxal phosphate or pyridoxamine phosphate restores activity, 2-oxoglutarate protects |
636606 |
2.6.1.7 | stable in phosphate buffer supplemented with pyridoxal phosphate |
636608 |