EC Number   |
General Stability |
Reference |
|---|
    2.6.1.42 | 2-mercaptoethanol protects against inactivation by sulfhydryl reagents |
640013 |
| 2.6.1.42 | caproate protects the enzyme |
640010 |
| 2.6.1.42 | enzyme is more stable in phosphate buffer than in Tris-Cl buffer, addition of glycerol increases stability |
640006 |
| 2.6.1.42 | not stable to freezing without addition of dithiothreitol |
640017 |
| 2.6.1.42 | on the basis of 5,5'-dithiobis(2-nitrobenzoic acid) titration of wild type BCATc, labeling of 6 thiol groups results in a loss of 60-70% of BCAT activity compared to that of the control enzyme incubated without 5,5'-dithiobis(2-nitrobenzoic acid) and assayed without dithiothreitol |
702237 |
| 2.6.1.42 | pure enzyme is not stable |
640032 |
| 2.6.1.42 | pyridoxal 5'-phosphate and mercaptoethanol stabilizes the heart enzyme, addition of 2-oxoglutarate does not increase its stability |
639993 |
