General Stability | Organism |
---|---|
enzyme is more stable in phosphate buffer than in Tris-Cl buffer, addition of glycerol increases stability | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxo-3-methylvalerate | measured in the forward reaction, concentrations higher than 1.0 mM inhibits the enzyme | Hordeum vulgare | |
2-oxoisocaproate | measured in the forward reaction, concentrations higher than 1.0 mM inhibits the enzyme | Hordeum vulgare | |
2-oxoisovalerate | measured in the forward reaction, concentrations higher than 1.0 mM inhibits the enzyme | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
2-Oxoisohexanoate | pH 8.4, 30°C, glutamate as amino group donor | Hordeum vulgare | |
0.2 | - |
2-oxo-3-methylpentanoate | pH 8.4, 30°C | Hordeum vulgare | |
0.4 | - |
2-oxoisopentanoate | pH 8.4, 30°C | Hordeum vulgare | |
0.8 | - |
L-leucine | pH 8.4, 30°C | Hordeum vulgare | |
0.8 | - |
2-oxoglutarate | pH 8.4, 30°C, L-valine as amino group donor | Hordeum vulgare | |
1.3 | - |
L-glutamate | pH 8.4, 30°C, 2-oxoisocaproate as amino group acceptor | Hordeum vulgare | |
1.5 | - |
2-oxoglutarate | pH 8.4, 30°C, leucine as amino group donor | Hordeum vulgare | |
1.8 | - |
2-oxoglutarate | pH 8.4, 30°C, isoleucine as amino group donor | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
gel filtration | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | Hordeum vulgare | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Hordeum vulgare | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Hordeum vulgare | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
barley, L. cv. Ingrid | - |
Purification (Comment) | Organism |
---|---|
partially | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Hordeum vulgare | - |
root | - |
Hordeum vulgare | - |
seedling | - |
Hordeum vulgare | - |
shoot | - |
Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
- |
Hordeum vulgare |
Storage Stability | Organism |
---|---|
-25°C, partially purified enzyme is stable for several weeks | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 2-oxoglutarate | - |
Hordeum vulgare | 2-oxopropanoate + L-glutamate | - |
r | |
L-alanine + 2-oxoisohexanoate | - |
Hordeum vulgare | pyruvate + L-leucine | - |
r | |
L-alanine + 2-oxoisopentanoate | - |
Hordeum vulgare | pyruvate + L-valine | - |
r | |
L-aspartate + 2-oxoglutarate | - |
Hordeum vulgare | oxaloacetate + L-glutamate | - |
r | |
L-aspartate + 2-oxoisohexanoate | - |
Hordeum vulgare | oxaloacetate + L-leucine | - |
r | |
L-aspartate + 2-oxoisopentanoate | - |
Hordeum vulgare | oxaloacetate + L-valine | - |
r | |
L-cysteine + 2-oxoglutarate | - |
Hordeum vulgare | 2-oxo-3-thiobutyrate + L-glutamate | - |
r | |
L-glutamate + 2-oxo-3-methylpentanoate | - |
Hordeum vulgare | 2-oxoglutarate + L-isoleucine | - |
r | |
L-glutamate + 2-oxo-isohexanoate | - |
Hordeum vulgare | 2-oxoglutarate + L-leucine | - |
r | |
L-glutamate + 2-oxo-isopentanoate | - |
Hordeum vulgare | 2-oxoglutarate + L-valine | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Hordeum vulgare | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + glyoxylate | - |
Hordeum vulgare | 2-oxoisohexanoate + glycine | - |
r | |
L-isoleucine + oxaloacetate | - |
Hordeum vulgare | 2-oxoisohexanoate + L-aspartate | - |
r | |
L-isoleucine + pyruvate | - |
Hordeum vulgare | 2-oxoisohexanoate + L-alanine | - |
r | |
L-leucine + 2-oxo-3-methylpentanoate | - |
Hordeum vulgare | 2-oxoisohexanoate + L-isoleucine | - |
r | |
L-leucine + 2-oxoglutarate | - |
Hordeum vulgare | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoisohexanoate | - |
Hordeum vulgare | 2-oxoisohexanoate + L-leucine | - |
r | |
L-leucine + 2-oxoisopentanoate | - |
Hordeum vulgare | 2-oxoisohexanoate + L-valine | - |
r | |
L-leucine + glyoxylate | - |
Hordeum vulgare | 2-oxoisohexanoate + glycine | - |
r | |
L-leucine + oxaloacetate | - |
Hordeum vulgare | 2-oxoisohexanoate + L-asparagine | - |
r | |
L-leucine + pyruvate | - |
Hordeum vulgare | 2-oxoisohexanoate + L-alanine | - |
r | |
L-methionine + 2-oxoglutarate | transaminated extremely poorly | Hordeum vulgare | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
r | |
L-valine + 2-oxo-3-methylpentanoate | - |
Hordeum vulgare | 3-methyl-2-oxobutanoate + L-isoleucine | - |
r | |
L-valine + 2-oxoglutarate | - |
Hordeum vulgare | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
L-valine + 2-oxoisopentanoate | - |
Hordeum vulgare | 2-oxoisopentanoate + L-valine | - |
r | |
L-valine + 4-methyl-2-oxopentanoate | - |
Hordeum vulgare | 2-oxoisopentanoate + L-leucine | - |
r | |
L-valine + glyoxylate | - |
Hordeum vulgare | 2-oxoisopentanoate + glycine | - |
r | |
L-valine + oxaloacetate | - |
Hordeum vulgare | 2-oxoisopentanoate + L-aspartate | - |
r | |
L-valine + pyruvate | - |
Hordeum vulgare | 2-oxoisopentanoate + L-alanine | - |
r | |
additional information | no significant activity with glycine | Hordeum vulgare | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
- |
Hordeum vulgare |
8.7 | - |
using valine in the reverse reaction | Hordeum vulgare |