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Literature summary for 2.6.1.42 extracted from

  • Yvon, M.; Chambellon, E.; Bolotin, A.; Roudot-Algaron, F.
    Characterization and role of the branched-chain aminotransferase (BcaT) isolated from Lactococcus lactis subsp. cremoris NCDO 763 (2000), Appl. Environ. Microbiol., 66, 571-577.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
nutrition widely used as starter in the cheese industry Lactococcus lactis

Cloned(Commentary)

Cloned (Comment) Organism
-
Lactococcus lactis
NCDO 763, gene cloned and sequenced with luciferase gene as the reporter Lactococcus lactis

General Stability

General Stability Organism
pure enzyme is not stable Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Lactococcus lactis
CoCl2
-
Lactococcus lactis
Cu2+
-
Lactococcus lactis
CuCl2
-
Lactococcus lactis
hydroxylamine
-
Lactococcus lactis
iodoacetamide
-
Lactococcus lactis
iodoacetic acid
-
Lactococcus lactis
phenylhydrazine
-
Lactococcus lactis
ZnCl2
-
Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, homodimer, SDS-PAGE Lactococcus lactis
64000
-
gel filtration Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate Lactococcus lactis
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate Lactococcus lactis
-
4-methyl-2-oxopentanoate + L-glutamate
-
r
L-valine + 2-oxoglutarate Lactococcus lactis
-
3-methyl-2-oxobutanoate + L-glutamate
-
r

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
subsp. cremoris NCDO 763 and TIL 46
-
Lactococcus lactis Q7BPX6 subsp. cremoris NCDO 763 and TIL 46, same nucleotide sequence accession number in DDBJ and EMBL >
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
94
-
substrate L-isoleucine + 2-oxoglutarate Lactococcus lactis

Storage Stability

Storage Stability Organism
-20°C, storage results in a very rapid loss of all activity Lactococcus lactis
0°C, storage results in a very rapid loss of all activity Lactococcus lactis
6°C, fairly stable for 1 week Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + pyruvate
-
Lactococcus lactis pyruvate + L-alanine
-
r
L-cysteine + 2-oxoglutarate
-
Lactococcus lactis 2-oxo-3-thiobutyrate + L-glutamate
-
r
L-isoleucine + 2-oxo-isopentanoate
-
Lactococcus lactis 2-oxoisohexanoate + L-valine
-
r
L-isoleucine + 2-oxoglutarate
-
Lactococcus lactis 3-methyl-2-oxopentanoate + L-glutamate
-
r
L-isoleucine + 2-oxoisohexanoate
-
Lactococcus lactis 3-methyl-2-oxopentanoate + L-leucine
-
r
L-isoleucine + 3-phenylpyruvate
-
Lactococcus lactis 2-oxoisohexanoate + L-phenylalanine
-
r
L-isoleucine + 4-methylthio-2-oxobutyrate
-
Lactococcus lactis 3-methyl-2-oxopentanoate + L-methionine
-
r
L-isoleucine + pyruvate
-
Lactococcus lactis 2-oxoisohexanoate + L-alanine
-
r
L-leucine + 2-oxo-isohexanoate
-
Lactococcus lactis 2-oxoisohexanoate + L-leucine
-
r
L-leucine + 2-oxoglutarate
-
Lactococcus lactis 4-methyl-2-oxopentanoate + L-glutamate
-
r
L-methionine + 2-oxoglutarate
-
Lactococcus lactis 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
r
L-phenylalanine + 2-oxoglutarate
-
Lactococcus lactis phenylpyruvate + L-glutamate
-
r
L-tryptophan + 2-oxoglutarate
-
Lactococcus lactis 2-oxo-3-indolylpropanoate + L-glutamate
-
r
L-tyrosine + 2-oxoglutarate
-
Lactococcus lactis p-hydroxyphenylpyruvate + L-glutamate
-
r
L-valine + 2-oxoglutarate
-
Lactococcus lactis 3-methyl-2-oxobutanoate + L-glutamate
-
r
L-valine + 2-oxoisopentanoate
-
Lactococcus lactis 2-oxoisopentanoate + L-valine
-
r
additional information with 2-oxoglutarate as acceptor L-alanine is no substrate, with L-aspartate as amino donor, oxaloacetate is no amino acceptor Lactococcus lactis ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 38000, homodimer, SDS-PAGE Lactococcus lactis

Synonyms

Synonyms Comment Organism
BcaT
-
Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
Lactococcus lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 partially inactivated by heating at 50°, 40% inactivation after 30 min, more than 80% inactivation after 30 min at 60°C Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Lactococcus lactis