Application | Comment | Organism |
---|---|---|
nutrition | widely used as starter in the cheese industry | Lactococcus lactis |
Cloned (Comment) | Organism |
---|---|
- |
Lactococcus lactis |
NCDO 763, gene cloned and sequenced with luciferase gene as the reporter | Lactococcus lactis |
General Stability | Organism |
---|---|
pure enzyme is not stable | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Lactococcus lactis | |
CoCl2 | - |
Lactococcus lactis | |
Cu2+ | - |
Lactococcus lactis | |
CuCl2 | - |
Lactococcus lactis | |
hydroxylamine | - |
Lactococcus lactis | |
iodoacetamide | - |
Lactococcus lactis | |
iodoacetic acid | - |
Lactococcus lactis | |
phenylhydrazine | - |
Lactococcus lactis | |
ZnCl2 | - |
Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, homodimer, SDS-PAGE | Lactococcus lactis |
64000 | - |
gel filtration | Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | Lactococcus lactis | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Lactococcus lactis | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Lactococcus lactis | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
subsp. cremoris NCDO 763 and TIL 46 | - |
Lactococcus lactis | Q7BPX6 | subsp. cremoris NCDO 763 and TIL 46, same nucleotide sequence accession number in DDBJ and EMBL > | - |
Purification (Comment) | Organism |
---|---|
- |
Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
94 | - |
substrate L-isoleucine + 2-oxoglutarate | Lactococcus lactis |
Storage Stability | Organism |
---|---|
-20°C, storage results in a very rapid loss of all activity | Lactococcus lactis |
0°C, storage results in a very rapid loss of all activity | Lactococcus lactis |
6°C, fairly stable for 1 week | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + pyruvate | - |
Lactococcus lactis | pyruvate + L-alanine | - |
r | |
L-cysteine + 2-oxoglutarate | - |
Lactococcus lactis | 2-oxo-3-thiobutyrate + L-glutamate | - |
r | |
L-isoleucine + 2-oxo-isopentanoate | - |
Lactococcus lactis | 2-oxoisohexanoate + L-valine | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Lactococcus lactis | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoisohexanoate | - |
Lactococcus lactis | 3-methyl-2-oxopentanoate + L-leucine | - |
r | |
L-isoleucine + 3-phenylpyruvate | - |
Lactococcus lactis | 2-oxoisohexanoate + L-phenylalanine | - |
r | |
L-isoleucine + 4-methylthio-2-oxobutyrate | - |
Lactococcus lactis | 3-methyl-2-oxopentanoate + L-methionine | - |
r | |
L-isoleucine + pyruvate | - |
Lactococcus lactis | 2-oxoisohexanoate + L-alanine | - |
r | |
L-leucine + 2-oxo-isohexanoate | - |
Lactococcus lactis | 2-oxoisohexanoate + L-leucine | - |
r | |
L-leucine + 2-oxoglutarate | - |
Lactococcus lactis | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-methionine + 2-oxoglutarate | - |
Lactococcus lactis | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
r | |
L-phenylalanine + 2-oxoglutarate | - |
Lactococcus lactis | phenylpyruvate + L-glutamate | - |
r | |
L-tryptophan + 2-oxoglutarate | - |
Lactococcus lactis | 2-oxo-3-indolylpropanoate + L-glutamate | - |
r | |
L-tyrosine + 2-oxoglutarate | - |
Lactococcus lactis | p-hydroxyphenylpyruvate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Lactococcus lactis | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
L-valine + 2-oxoisopentanoate | - |
Lactococcus lactis | 2-oxoisopentanoate + L-valine | - |
r | |
additional information | with 2-oxoglutarate as acceptor L-alanine is no substrate, with L-aspartate as amino donor, oxaloacetate is no amino acceptor | Lactococcus lactis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, homodimer, SDS-PAGE | Lactococcus lactis |
Synonyms | Comment | Organism |
---|---|---|
BcaT | - |
Lactococcus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Lactococcus lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | partially inactivated by heating at 50°, 40% inactivation after 30 min, more than 80% inactivation after 30 min at 60°C | Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Lactococcus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Lactococcus lactis |