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EC Number
Activating Compound
Commentary
Reference
4.2.1.84
acrylonitrile
below
673098
4.2.1.84
activator 17K
the putative activator (17K, MW 17 kDa) gene next to the beta-subunit gene is proven to be important for the functional expression of recombinant NHaseK in Escherichia coli
747361
4.2.1.84
crotonamide
induces high and low molecular mass forms of the enzyme
648444
4.2.1.84
cyclohexanecarboxamide
induces the low molecular mass form of the enzyme
648444
4.2.1.84
dimethylformamide
addition to increase the accessibility of nitrile groups at concentration no higher than 0.5% is beneficial for activity
648456
4.2.1.84
glycine-NaOH
activates
648422
4.2.1.84
GroEL-ES
Escherichia coli chaperones GroEL-ES work well in Rhodococcus and simultaneously possess protein-folding assistance functions and the ability to stabilize and reactivate the native nitrile hydratase
748384
4.2.1.84
light
-
648430
4.2.1.84
light
effective wavelength: 370 nm
648423
4.2.1.84
light
restores activity inhibited by NO
648446
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